Agarose Gel Electrophoresis Lab quiz
What is alkali?
A chemical compound that turns acids blue
Agarose gel electrophoresis
A powerful and widely used analytical method that separates molecules on the basis of charge, size, and shape
Autoradiograph
After washing, remove excess probe, the sheet is placed on X ray film. After processing, the hybridized fragments appear as bands
Agarose is made from...
Agar, a product made form algae
How are electrophoresis samples prepared?
By mixing them with a dense solution such as glycerol or sucrose. This makes samples denser than the buffer, allowing them to sink thru and settle into walls
How is agarose gel made?
Dissolving agarose powder in a buffer solution by boiling the solution. Solution cools and poured into molds. The gel is then submerged into a buffer chamber.
Why is the AGE method useful?
Good at separating charged biomolecules such as DNA, RNA, and proteins
What does buffer salt do?
Makes water a better conductor of electricity and controls ph
What influences the rate at which molecules migrate?
Sieving properties of the gel
What is a dna probe?
Single stranded dna fragment which is complementary to repetitive sequences scattered throughout genome.
The higher the applied voltage...
The faster the molecules travel
Which molecules move faster?
The smaller ones can weave through pores. Sphere and compact shapes move quicker than rod shaped.
How do charges molecules enter into the gel?
Through walls of the wells of the porous gel
What is buffer used for in gel chambers?
To add electrolytes so it's easier to see when lit up
What color moves the fastest through the gel?
Yellow dye