AHHHHHHH

Genomics is the molecular analysis of ______.

the entire genetic composition of a species

True or false: Correlations among cytogenetic, linkage, and physical maps are constant from one region of a chromosome to another and from one species to another.

False

During FISH, how are fluorescent molecules localized to specific DNA sequences on chromosomes?

Fluorescently labeled avidin binds to biotin that is attached to a single-stranded DNA probe.

Which of the following is an accurate description of shotgun sequencing?

Random DNA fragments are sequenced, and overlapping parts of the sequence are identified.

Which type of molecular marker does NOT vary in size between individuals?

SNP

A genetic distance of 1 mu corresponds to ______.

a variable physical distance that depends on the recombination frequency

In Western blotting, what probe is used to detect the protein of interest?

an antibody

Developing technology for the management of human genome information was one of the goals of the Human Genome Project. To meet this goal, the Human Genome project developed ______.

analytical tools for interpreting genome information

If a polymorphic marker is close to a disease-causing allele, then the marker will ______ the disease-causing allele.

be linked to

In FISH, the single-stranded DNA probe is synthesized with nucleotides tagged with the small molecule ______, and a fluorescent molecule is attached to the protein _________.

biotin, avidin

Which of the following processes is used for physical mapping?

cloning fragments of chromosomal DNA

The distance between two different microsatellites can be determined by calculating the frequency of ______ between them.

crossing over

In the most common method of in situ hybridization, which method is used to detect the binding of DNA probes to specific regions of chromosomes?

flouresence

The study of the interactions of many genes and the ways that the components of a genome interact to produce an organism's traits is called ___________ genomics

functional

A research endeavor with the goal of determining the sequence of DNA bases in an organism's entire genome is called a(n) _________-________ project.

genome sequencing

Which process will result in a point mutation in the target gene when using the CRISPR-Cas technology for gene editing?

homologous recombination repair

If a geneticist states that a polymorphic marker is linked to a disease-causing allele, this means that the marker ______.

is close to the disease-causing allele

The tendency of different microsatellites to be transmitted together to the same offspring indicates that the microsatellites are ______.

linked

The site on a genetic map where a specific gene or other DNA sequence is found is called its

locus

The (CA)n dinucleotide is an example of a(n) _______found in thousands of copies in the _______genome.

microsatellite, human

This technique is used to identify a specific RNA molecule within a mixture of RNA molecules

northern

A microsatellite can be amplified by PCR using primers that are located ______.

on both sides of the microsatellite

Which mapping strategy involves cloning many pieces of DNA and characterizing their sizes and relative locations along a chromosome?

physical mapping

Geneticists use several types of mapping strategies to identify the locations of genes. The approach that relies on DNA cloning techniques and allows computation of distances in base pairs is ________ mapping. The technique that is based on recombination frequencies between genes is ______ mapping. The technique that locates genes relative to bands on chromosomes that are visible by microscopy is mapping ________.

physical, linkage, cytogenetic

To analyze a microsatellite sequence, a scientist must first amplify it by PCR. This requires the use of PCR ______ that flank the repeated sequence of the microsatellite.

primers

One of the methods that is used for genome-sequencing projects involves the sequencing of randomly generated DNA fragments followed by identification of overlapping regions in the DNA sequence. This method is called _________ sequencing.

shotgun

When the CRISPR-Cas system is used for gene mutagenesis, tracrRNA and crRNA are linked together in a molecule called _______ ________ the RNA (sgRNA).

single guide

Linked microsatellites may sometimes segregate due to crossing over. By determining the frequency of crossing over between two microsatellites, a geneticist can determine ______.

the distance between them

In the Western blot, to what is the enzyme that will give the colored reaction coupled?

the secondary antibody

What is the goal of a genome-sequencing project?

to determine the DNA sequence of the entire genome of a given species

Which of the following causes of genetic disorders can be detected with FISH analysis?

translocations, deletions, duplications

Because of the experimental strategy used for shotgun sequencing, researchers using this strategy may ______.

waste time sequencing the same region of DNA many times

In the Western blot, what binds to the protein of interest?

the primary antibody