BIO 10 Electrophoresis and CSI Wildlife Quiz

From CSI Wildlife, Case One, Review section: When choosing loci to serve as markers for DNA analysis, which of the following is TRUE? a) The loci should have very low variability b) The loci should be able to be amplified c) Only one locus should be chosen

The loci should be able to be amplified

T/F: DNA fragments that are used in gel electrophoresis are segments of repeated bases and the number of repeats is what differs between individuals

True

T/F: Although gel electrophoresis is often used to compare DNA from a suspect to DNA recovered from a crime scene, the odds of two different people having the same bands on the gel are high, so the test is not that useful.

False

From CSI Wildlife, Case One, Technique section: What was the largest DNA fragment for the elephant on the right? a) Approximately 65 base pairs b) Approximately 70 base pairs c) Approximately 181 base pairs d) Approximately 190 base pairs e) Approximately 200 base pairs

Approximately 190 base pairs

T/F: Gel electrophoresis separates charged molecules by size because larger molecules move faster and farther than smaller molecules through the gel

True

From CSI Wildlife, Introduction Video: Choose all of the statements below that are true. a) A Keystone species is one that is essential to maintaining the ecosystem. b) Approximately 90% of the elephant population is killed each year for their tusks. c) Elephant tusks are primarily used for medicinal purposes. d) Steve Wasser uses DNA from tusks taken from poachers to find out where the elephant originated.

a) A Keystone species is one that is essential to maintaining the ecosystem d) Steve Wasser uses DNA from tusks taken from poachers to find out where the elephant originated.

From CSI Wildlife, Case Two, Introduction section: Choose all of the TRUE statements a) In Case One, we were matching DNA from an ivory sample to one specific elephant. b) In Case Two, we were comparing DNA profiles of different elephant populations. c) DNA profiles for populations across Africa were made using blood samples from elephants. d) The further apart two populations are geographically, the more different their DNA profiles are.

a) In Case One, we were matching DNA from an ivory sample to one specific elephant. b) In Case Two, we were comparing DNA profiles of different elephant populations. d) The further apart two populations are geographically, the more different their DNA profiles are.

From CSI Wildlife, Case Two, for the gel in the Applications section: Choose all of the TRUE statements. a) The lane for the ivory sample has two bands because that lane is analyzing one marker from one elephant that is heterozygous for that marker. b) Lanes A and B have multiple bands because those lanes include many different elephants.

a) The lane for the ivory sample has two bands because that lane is analyzing one marker from one elephant that is heterozygous for that marker. b) Lanes A and B have multiple bands because those lanes include many different elephants.

From CSI Wildlife, Case Two, Finding a Location section: Choose all of the TRUE statements a) We first eliminated forest elephants. b) We eliminated North before South. c) The marker in the blue box was used to eliminate the Northern population. d) Dr. Wasser linked these tusks to the Western Africa elephant populations.

a) We first eliminated forest elephants. b) We eliminated North before South.