Biosafety (Part 1)

C. Grab a biological spill kit, cover the spill with wipes or absorbent towel until dry Instructions in order: 1. Cover the spill immediately with absorbent material then flood with 0.5% Sodium Hypochlorite (NaClO) solution 2. Notify the co-worker and safety officer immediately 3. If PPE contact is suspected, decontaminate affected area of the PPE the leave the area, doff PPE and don a new set 4. The staff should mark the area with a warning sign

If a chemical spill occurs inside the laboratory, what steps would immediately be taken? A. Inform the supervisor immediately to report the incident B. Leave the room, evacuate the laboratory and notify the safety authorities C. Grab a biological spill kit, cover the spill with wipes or absorbent towel until dry D. Secure other chemicals nearby and clean the spilled area with a moppler

C. A or B, as appropriate

If an exposure incident occurs, the exposed student/researcher should: A. Wash the exposed skin with soap and water, flush other exposed areas with water, and inform the instructor B. Seek medical treatment at the location identified in your exposure control plan C. A or B, as appropriate D. Go on as if nothing happened to avoid problems

B. Mask

In doffing PPE, what must be the last protective equipment that a student should remove? A. Gloves B. Mask C. Eye goggles D. Gown

D. Gown

In donning PPE, what must be the first protective equipment that a student should wear? A. Gloves B. Mask C. Eye goggles D. Gown

C. Elimination or substitution

In the hierarchy of controls, which is the most effective A. PPE B. Engineering controls C. Elimination or substitution D. Administrative controls

B. Using needle designs engineered to minimize needle stick injuries Explanation: Medical sharps that incorporate features or mechanisms to prevent or minimize the risk of accidental injury.

Safe sharps practices refers to: A. Using plastic wares instead of breakable glasswares B. Using needle designs engineered to minimize needle stick injuries C. Discouraging the practice of recapping needles D. All of these

C. Protect your clothes off from spills and contaminants

The main purpose of a laboratory gown/coat is to: A. Protect your clothes from being ruined B. It is one of the standard operating procedures in the laboratory C. Protect your clothes off from spills and contaminants D. To help maintain the cleanliness inside the laboratory

B. False

The wearing of Personal protective equipment (PPE) is worn upon the discretion of the student or researcher doing the work in the laboratory. A. True B. False C. Depending on the kind of research work the student or researcher will perform D. For pathogenic microorganisms to be manipulated only

B. Associated with human disease which is rarely serious and for which preventive or therapeutic interventions are often available

A Biosafety Level 2 (BSL-2) lab works with agents that may be described as: A. Not associated with disease in healthy adult humans B. Associated with human disease which is rarely serious and for which preventive or therapeutic interventions are often available C. Associated with serious or lethal human disease for which preventive or therapeutic interventions may not be available but are not readily transmissible to others (high individual risk but low community risk) D. Laboratory personnel are under medical surveillance and could receive immunizations for microbes they work with

D. All of these

Acceptable alternatives to Bunsen burner use in the biological safety cabinet (BSC) include: A. Disposable loops, spreaders, or glass beads B. Bacticinerator C. Wrist-operated Touch-O-Matic burner D. All of these

D. B and C

At work in the laboratory, what important house rules must be complied by the students? A. Eating, drinking, smoking or applying cosmetics in the work area B. Washing of hands before leaving the work area C. Wearing the appropriate personal protective equipment (PPE) D. B and C

D. All of these

Biohazard labels or stickers are required to be posted on: A. Storage and transport containers/equipment used for infectious organisms B. Biomedical waste containers C. Contaminated glasswares D. All of these

C. Yearly

Calibration and certification of the Biological Safety Cabinets used for infectious / potentially infectious material must be done at least: A. Daily B. Monthly C. Yearly D. Once

D. A and B

Ethanol or isopropyl alcohol solutions are not a preferred disinfectant, because: A. They require a long contact time and have a fast evaporation rate B. They do not have broad spectrum disinfectant capability C. They are not highly flammable D. A and B

A. Once a day Explanation: Make a new diluted bleach solution daily. Bleach solutions will not be as effective after being mixed with water for over 24 hours.

Disinfectants like zonrox or lysol should be diluted with water and prepared A. Once a day B. Once a week C. Once a month D. When the bottle is empty

C. Empty the sharps container into a properly lined, leak proof, puncture proof container.

Once you notice that the sharps container is getting full, you should: A. Empty the sharps container into a biohazard bag B. Obtain a new one, close the lid on the full one and arrange to have it safely transported for incineration C. Empty the sharps container into a properly lined, leak proof, puncture proof container D. Recap the needles and throw them in the regular trash

D. All of these

Open flames are not allowed in a biological safety cabinet because A. They disrupt airflow patterns that protect user and product B. They can damage HEPA filters C. They pose a fire hazard D. All of these

B. False

Wearing of open sandals inside the laboratory is allowed as long as socks are worn as well. A. True B. False C. Depending on the brand of the sandals D. Depending on the policy of the laboratory

A. To protect the sample, the person doing the work, and the environment

What is the purpose of a biosafety cabinet in a microbiology laboratory? A. To protect the sample, the person doing the work, and the environment B. To prevent the sample from contaminating the environment C. To provide a better place and temperature for microbes to grow D. To sterilize the samples

D. All of these

Which of the following is an ideal material for the containment of biohazardous wastes: A. The garbage bin must be hard-sided B. The garbage bin must have a foot operated lid C. The garbage bin must be easily decontaminated D. All of these

D. Food residues

Which of the following materials may be disposed of in the regular trash? A. Animal tissue, human blood, or body fluids B. Used masks and gloves C. Broken glass D. Food residues

B. Put the materials in a sturdy, leak-proof secondary container

Which of the following must be practiced when transporting a biological sample from one laboratory to another? A. Wear gloves and carry the material in your hands B. Put the materials in a sturdy, leak-proof secondary container C. Wear a laboratory gown and place the transport materials in your pocket D. Cells in cell culture flasks and /or petri dishes are fine for transport

C. Escherichia coli K-12

Which of the following organisms are safe to be manipulated in a BSL-1 laboratory? A. Staphylococcus aureus B. Pseudomonas aeruginosa C. Escherichia coli K-12 D. Candida albicans

D. All of these

Which of the following practices should be utilized when working in a biological safety cabinet? A. Disinfect all surfaces of the BSC before and after work B. Disinfect all items which go into and come out of the BSC C. Allow the BSC to operate before work begins and after work ceases D. All of these

C. Biological safety cabinet (BSC)

Which of the following protect both the user and the material that is handled inside the cabinet A. Fume hood B. Clean bench C. Biological safety cabinet (BSC) D. All of these

D. All of these

You should alert your instructor and reassess health risks involved with BSL-1 and BSL-2 material that you are working with when: A. You have a cold or the flu B. You are pregnant C. You have been diagnosed with or suspect that you may have an immune disorder D. All of these