Biotechnology

Plasmid DNA is circular DNA that is not part of a _____________.

Chromosome

Gene __________ is the process of isolating and making copies of a gene.

Cloning

What is recombinant DNA?

DNA that results when two Organisms combine

What are the three steps of PCR?

Denturing, Annealing, Extension

The use of biotechnology has raised a number of _________, legal, and social issues.

Ethical

T/F The three steps of PCR are denaturing, annealing, and elongation.

False ( Extension Not Elongation)

T/F Denaturing DNA occurs at room temperature.

False ( Nothing in PCR involves room temperature)

T/F When genes are cloned, DNA polymerase is used to join two pieces of DNA together.

False (DNA Ligase)

T/F Gene cloning involves three steps: isolation, transformation, and selection.

False (Ligation goes in between Transformation and selection)

T/F Gene cloning is the process of isolating and making copies of a chromosome.

False (We clone genes)

What is gene cloning?

Gene cloning is the process of isolating and making copies of a gene.

Transgenic crops are genetically modified with new ___________ that code for traits useful to humans.

Genes

What are the four steps of gene cloning?

Isolation, Ligation, Transformation, Selection

DNA __________ can join together an isolated gene and plasmid DNA.

Ligase

The__________ makes many copies of a gene or other DNA segment.

PCR (polymerase chain reaction)

___________ DNA is made by combining DNA from two different sources.

Recombinant

Why is it important to select for transformed bacteria?

So you make sure that the bacteria you want growing

What is PCR? Why is PCR done?

The polymerase chain reaction (PCR) makes many copies of a gene or other DNA segment. This might be done in order to make large quantities of a gene for genetic testing.

5. Gene cloning involves four steps: isolation, ligation, ____________, and selection.

Transformation

T/F . The enzyme Taq Polymerase can work at high temperatures.

True

T/F Biotechnology has raised ethical questions.

True

T/F Insulin was the first human protein to be produced by gene cloning.

True

T/F Medicine and agriculture are two major fields that use biotechnology.

True

T/F Recombinant DNA is made from joining DNA from different sources.

True

T/F The polymerase chain reaction makes many copies of a gene or other DNA segment.

True

T/F The purpose of biotechnology is to create organisms that are useful to humans.

True

T/F Transgenic crops have been created that make some food taste better.

True

T/F Two common biotechnology techniques are gene cloning and genetic engineering.

True

The first step of the PCR process is _________.

denaturing

Changing an organism by transforming with recombinant DNA is known as genetic _____________.

engineering