Blood Bank ASCP 4

A refrigerator used to store whole blood must be able to maintain a temperature in the ranges of:

1 - 6 C

A D negative mother gives birth to a D positive child. The result of the fetal maternal hemorrhage test is positive. The Kleihauer-Betke test is performed next to quantify the volume of the fetal bleed. The result of this test indicates that the mother has been exposed to 20 mL of fetal blood. How may 300 microgram vials of RhIg should the mother receive?

2 vials

What is the proper storage temperature for thawed cryoprecipitate?

20 - 24 C

After frozen RBC have been thawed and washed, they must be used within how many hours?

24 hours

Which of the following donors could be accepted for blood donations?

24 year old man who had a tooth extraction two months ago

What is the maximum interval during which a recipient sample may be used for compatibility testing if the patient has recently been transfused or was pregnant within the last 3 months?

3 days

Severe blood loss causing circulatory collapse and shock usually first occurs when what percentage of blood is lost?

30 - 40%

A standard blood donation of 450 - 500 mL requires a minimum anticoagulant preservative solution volume of:

63 mL

Which of the following statements are true about anti-H?

Anti-H reacts more strongly with group A2 cells than with group A1 cells

The plant lectin obtained from the Dolichos biflorus plant will agglutinate which of the following?

A1 cells

What ABO phenotype will exist in an individual that inherits an A gene from one parent and a B gene from the other?

AB

What are the possible ABO genotypes of offspring from parents whose genotypes are OO and AB?

AO and BO

Which one of the following test best correlates with the severity of HDFN?

Amniotic fluid bilirubin

Which of the following individuals is a candidate to receive RhIg?

An Rh negative mother upon delivery of her first child

A febrile hemolytic transfusion reaction is characterized by which of the following?

An increase in temperature of >1C above 37 C during transfusion

A patient demonstrates with a positive antibody screen. You need to identify the unknown antibody, so you decide to perform a full work up using untreated and ficin treated panels. The results are as follows... Which antibody may be present?

Anti-Fy(a)

A 27 year old female presents to her physician after suffering a spontaneous abortion. Though rare, which antibody may be the etiologic agent?

Anti-P

Which of the following best describes a transfusion reaction?

Any adverse event associated with the transfusion of blood components

A 9 year old male in Alaska presents with hemolysis and hematuria. The patient history reveals that he recently recovered from a flu like virus. The ABO type is A+ and DAT reacts 1+. What blood group antibody should be suspected?

Auto Anti-P

Which is in the correct order from the lowest concentration of H antigen to the highest concentration?

Bombay > A1B > A1 > A2B > B > A2 > O

An aliquot of AS1 RBC is being prepared from an intact packed cell unit using a sterile connection device. During the process of preparing an aliquot, the sterile device fails and blood drips onto the counter. What should be done with the primary unit?

Change the expiration date to 24 hours

Which of the following methods are a non-invasive way to reliably predict anemia in the fetus?

Color Doppler

A D positive mother with a D negative fetus eliminates the possibility of HDFN due to the:

D antigen

Louisa is a healthy 24 year old woman that wants to donate blood today. Her hemoglobin, temperature, blood pressure, and pulse are all within acceptable limits for donating. She is in good health and not taking any medications. She recently divorced her spouse who was a hemophiliac that regularly received factor concentrates. Can Louisa donate blood today?

Maybe, depending on Louisa's last sexual contact with her ex-spouse

What is the most common Rh haplotype among whites?

DCe

Storage lesion, or the biochemical changes in RBC during storage, will result in:

Decreased plasma pH

What is the Kleihauer-Betke test used for?

Differentiate between maternal and fetal red cells

Platelet products will be prepared from a whole blood donation. What must be used to prevent potential contamination of the platelets with skin cells and bacteria?

Diversion pouch

Which of these methods measures fetal hemoglobin or D positive red cells or both to evaluate fetomaternal hemorrhage?

Flow cytometry

What sugar configuration is necessary as a base for attachment of other sugars to produce ABO antigens?

Galactose plus fructose

Many RBC processes such as binding, transporting and releasing oxygen do not require energy. Energy however, is required for cellular viability. How does the RBC obtain most of its energy?

Glycolytic pathways

Antibodies to which of the following blood components is the most frequent cause of febrile non-hemolytic transfusion reactions?

Granulocytes

Certain clinical conditions such as HDFN, hemolytic transfusion reaction and autoimmune hemolytic anemia result from the attachment of antibodies or complement to human RBC. What is this process called?

In vivo sensitization

Which of the following characteristics is consistent with the Kidd blood group system?

Jk(a-b-) red cells are resistant to lysis in 2 M urea

Delayed hemolytic transfusion reactions are usually caused by antibodies directed against what blood group system?

Kidd

All of the following would be included in a hemolytic transfusion reaction investigation, except?

Leukocyte antigen studies

Which of the following set of conditions would not allow HDFN to occur as a result of Rh incompatibility?

Mother Rh negative, father Rh negative

If a patient is determined to have type AB blood, what antibody would be found in his serum?

No Anti-A and no Anti-B antibodies

What are the possible ABO genotypes of offspring of parents whose genotype is AO and BO?

OO AO BO AB

While working at a blood bank lab, you hear chimes over the hospital loud speaker announcing the birth of a baby. Thirty minutes later you receive a cord blood specimen that you identify as O positive. You previously received the mother's specimen and she was O negative with a negative antibody screen. What is the next action?

Perform a fetal bleed screen

What is the correct way to thaw FFP?

Placing the FFP in a 37 C water bath

Rh Immune Globulin is used to:

Prevent the formation of anti-D

When performing an antibody screen, both screen cells are 4+ at IS and W+ at AHG. The antibody panel shows 4+ reactions at IS and W+ reactions at AHG and there is no specific match to the reaction pattern. The autocontrol is negative. What would be a logical next step?

Repeat testing using warmed patient sample/reagent and just do AHG reading

Donated red cell units contain which substance to stabilize the pH of the unit during storage?

Sodium biphosphate

Which of the following is the most important first step to take when a patient is transfused with uncrossmatched RBC that turn out to be incompatible?

Stop any transfusion in progress

What is the most likely explanation for the following results >> Anti-A 1+ Anti-B 0 A1 cells 1+ B cells 4+

Subgroup of A with Anti-A1

Phenotype refers to:

The expression of traits

What is considered the cause of uticarial transfusion reactions?

Transfusion of certain food allergens or drugs in plasma products

A patient who demonstrates a history of an allergic reaction rom RBC transfusions will benefit from receiving which red cell component?

Washed RBC

Transfusion of which blood product is most likely to cause circulatory overload in patients?

Whole blood