Blood Transfusion

the following family study is performed- Mother: K+k+ Father: K-k+ Child 1: K+ k- Child 2: K-k+ all other indications are that these children are both the products of this mating. possible explanations for these results would include: a- dominant inhibitor gene has been passed to child 1 b- father has one k gene and one K0 gene c- father has one McLeod phenotype (Kx null) d- Mother has cis-Kk gene

B- father has one k gene and one K0 gene

biochemical changes occur during the shelf life of stored blood. which of the following is a result of this "storage lesion"? a- increase in pH b- increase in plasma K c- increase in plasma Na d- decrease in plasma Hgb

B- increase in plasma K (leaks out of red blood cells)

the fial product was prepared with a PRP spin time of 2min at 2500rpm. to increase the % plt yield in the final product, one would... a- increase the time and/or rpm for the first spin b- increase the time and/or rpm on the second spin c- decrease the time and/or rpm for the first spin d- decrease the time and/or rpm on the second spin

B- increase the time and/or rpm on the second spin

it is generally asymptomatic but has a very high carrier rate (70-80% have chronic infections). about 10% of the carrieers develop cirrhosis or hepatocellular carcinoma. these statements are most typical of which of the following transfusion- transmitted infections? a-HAV b- HBV c-HCV d- HEV

C- HCV (others not associated with hepatocellular carcinoma or cirrhosis of liver in many of the chronically infected patients)

if a person has a genetic makeup Hh, AO, LeLe, sese, what substances will be found in the secretions? a- A substance b- H substance c- Lea substancee d- Leb substance

C- Lea---> both LeLe and SeSe gene need to be present to secrete Leb

previous records of patients ABO and Rh types must be immediately available for comparison with current test results a-for 6mths b- 12mths c- 10yrs d-indefinitely

B- 12mths

platelet concentrate should be stored at a- -18C b- 20-24C c- -65C d- 1-6C

B- 20-24C

allowable shelf life of blood for transfusion therapy with CPD (citrate phosphate dextrose) a- 35 days b- 21 days c- 1 year d- 42 days

B- 21 days

in order to meet current AABB standards for leukocyte reduction to prevent HLA alloimmunization or CMV transmision, the donor unit must retain at least ______ of the original red cells and leukocytes must be reduced to less than_______ a- 85%, 5x10^8 b- 80%, 5x10^6 c- 75%, 5x10^5 d- 70%, 5x10^4

B- 80%, 5x10^6

which of the following antibodies has been clearly implicated in transfusion reactions and hemolytic disease of the newborn? a-Anti-I b- Anti-K c-Anti-Lea d- Anti-N

B- Anti-K (Kell Kills)

a white females rbc gave the following reactions upon phenotyping: D+ C+ E- c+ e+. which of the following is the mst probable Rh genotype? a-DCe/Dce b- DCe/dce c- DCe/DcE d- Dce/dCe

B- DCe/dce-----> based off the freq. of genes. the genes that code for the haplotypes DCe and dce are high in white pop'n. (31.1%)

a patients RBC are being typed for the Fya antigen. which of the following is the proper cell type of choice for a positive control of the anti-Fya reagent? a- Fy(a+b-) b- Fy(a+,b+) c- Fy(a-b+) d- Fy(a-b-)

B- Fy(a+b+) (for control purposes, the cell should have the weakest expression of the antigen in question--dosage effect--RBC might be mistyped if reagent cant pick up small amounts of antigens in heterozygous people)

lymphocytotoxicity testing can be used to detect the presence of antibodies to a- Wra b- HLA antigens c- Bga, Bgb, and Bgc d- JMH antigen

B- HLA antigens (read book for further detail)

a patient arrives in the OB clinic 3mths pregnant. this is her first pregnancy and has never been transfused. her prenatal screen includes the following: ABO- 0 SC 1- IS/37/AHG 4+ SC II- IS/37/AHG 4+ AC- 0 check cells- pass if the patients RBC were tested against anti-H lctin and did not react, this person would be identified as a(an)? a- acquired B phenotype b- Oh phenotype c- secretor d- subgroup of A

B- Oh phenotype----Bombay individual

lectins are useful in determining the cause of abnormal reactions in BB serology. these lectins are frequently labeled as anti-H, anti-A, ect. the nature of these lectins is explained by which of the following?' a- an early form of monoclonal antibody produced in non-vertebrates b- a plant substance that chemically reacts with certain RBC antigens c- naturally occurring antibodies in certain plants d- the ability of plants to respond to RBC antigens by antibody production

B- a plant substance that chemically reacts with certain RBC antigens

if the patient has an Rh genotype DCe/DCe and receives a unit of RBC from a DCe/dce individual, what Rh antibody might the patient develop? a- anti-C b-anti-c c- anti-d d- anti-E

B- anti-c (antigen is present in >80% pop'n)

shelf life of FFP, thawed prepared from whole blood a- 1 year b- 2 years c- 32 days d- 24 hours

D- 24hours

allowable shelf life of blood for transfusion therapy with AS-1 (Adsol) a- 35 days b- 21 days c- 1 year d- 42 days

D- 42 days

shelf life of platelet concentrate in PL-732 (with agitation), prepared from whole blood a- 10 days b- 42 days c- 1 year d- 5 days

D- 5 days

a centrifuge used for platelet preparation has been returned after a major repair. a unit of whole blood(450mL; platelet count 200,000/uL) is selected for calibration of platelet production. the PRP contains 250mL with a plt count of 300,000/uL. the final plt concentrate prepared from PRP contains 50mL with a plt count of 900,000/uL what is the % yield of platelets in the final product? A- 30% b- 45% c- 50% d- 60%

D- 60% ( 900,000 x 50 (=45,000,000) / 300,000 x 250 (= 75,000,000) = 0.6 x 100 = 60%)

the last unit of autologous blood for an elective surgery patient should be collected no later than______ hrs before surgery a- 24 b- 36 c- 48 d- 72

D- 72 (allows for rejuvination of rbc--(retics))

red blood cells, liquid, should be stored at temp

1-6C

questions 94-96 refer to the book for red cell panel

94-A 95- B 96- C

FFP should be stored at a- -18C b- -65C c- 20-24C d- 1-6C

A- -18C or colder

cryoprecipitate should be stored at a- -18C b- -65C c- 20-24C d- 1-6C

A- -18C or colder

shelf life of cryoprecipitate prepared from whole blood a- 1 year b- 10 years c- 2 years d- 5 days

A- 1 year

allowable shelf life of blood for transfusion therapy with CPDA-1 (citrate phosphate dextrose adenine) a- 35 days b- 21 days c- 1 year d- 42 days

A- 35 days

which of the following viruses resides exclusively in leukocytes? a-CMV b- HIV c- HBV d- HCV

A- CMV

A satellite bag containing 250mL of fresh plasma is selected for quality control of cryoprecipitate production. cryprecipitate is prepared according to standard operating procedures. the final product has a total volume of 10mL. the factor VIII assays are 1UL/mL before and 9IU/mL after preparation what is the per does this product meet AABB standards for cryoprecipitate production? a- yes b- no; the percent recovery is too low c- no; the final factor VIII is too low d- data are insufficient to calculate

A- Yes (product contains 90IU/mL; minimum of 80IU/mL per container must be present in final product)

an individual of the dce/dce genotype given dCe/dce blood has an antibody response that appears to be anti-C plus anti-D. what is the most likely explanation? a- the antibody is anti-G b-the anti-body is anti-partial D c- the antibody is an anti Cw d- the reactions were read incorrectly

A- anti-G---> RBC that have either C or D antigen, also have the G antigen. when anti-G is made, it is capable of reacting with the G antigen on both C-pos and D-pos RBC, therefore appearing to be anti-C plus anti-D antibodies. the immunizing red blood cells were D-neg and C pos. therefore, what appears to be a a combination of anti-C and anti-D is anti-G or a combination of anti-C and anti-G

donors who have received blood or blood products within the last 12mths of when they desire to donate are differed to protect the recipient because the.... a- blood could have transmitted hepatitis or HIV b- blood may have two cell pop'n c- donor may not be able to tolerate the blood loss d- donor red cell hemoglobin level may be to high

A- blood could have transmitted hepatitis or HIV

which of the following tests on donor red blood cells must be repeated by the transfusing facility when blood was collected and processed by a different facility? a-confirmation of ABO group and Rh type of blood labeled D-neg b- confirmation of ABO group and Rh type c- weak D on D-neg d- antibody screening

A- confirmation of ABO group and Rh type of blood labeled D-neg

the addition of adenine in the anticoagulant-preservative formulation aids in.. a- maintaining the ATP levels for red cell viability b- maintaining platelet function in stored blood c- reducing the plasma K levels during storage d- maintaining 2,3-BPG levels for oxygen release to the tissues

A- maintaining ATP levels for red blood cell viability during storage

If a D-positive person makes anti-D, this person is probably.. a-partial D b- D-negative c- weak D as position effect d- weak D due to transmissible genes

A- partial D

a serum containing anti-k is not frequently encountered. this is because a- people who lack the k antigen are rare b-- people who possess the k antigen are rare c- the k antigen is not a good immunogen d- kell null people are rare

A- people who lack the k antigen are rare (k antigen is a high frequency antigen, present in greater than 99% of the random pop'n; probability of encountering a k-neg and capable of producing the corresponding antigen after RBC stimulation is very rare; Kell system antigens are good immunogens, second only to those of the Rh sys.)

which of the following is characteristic of the Xga blood group system? a- the Xga antigen has higher frequency in woman than men b- the Xga antigen has higher freq. in men than women c- the Xga antigen is enhanced by enzymes d- anti-Xga is usually a saline-reacting antibody

A- the Xga antgen has higher freq. in woman than men. (produced by a gene on the X chromosome- detected by antiglobulin test and the antigenic activity is depressed by enzymes)

an antigen-antibody reaction alone does not cause hemolysis. which of the following is required for rbc lysis? a-albumin b- complement c- G6PD d- AHG

B complement

how many genes encode the following Rh antigens: D, C, E, c, e? a- one b-two c- three d- four

B two genes: RHD (contRols expression of D) and RHCE (determines C,E,c,e)

FROZEN RBC should be stored at a- -18C b- -65C c- 20-24C d- 1-6C

B- -65C or colder

a centrifuge used for platelet preparation has been returned after a major repair. a unit of whole blood(450mL; platelet count 200,000/uL) is selected for calibration of platelet production. the PRP contains 250mL with a plt count of 300,000/uL. the final plt concentrate prepared from PRP contains 50mL with a plt count of 900,000/uL does this product meet AABB standards for plt concentrate production? a-yes b- no; the count on the final product is too low c- no; % recovery in the PRP is too low d- data are insufficient to calculate

B- no; the count on the final product is too low (needs to be min. of 5.5x10^10/uL; this product is 4.5x10^10/uL)

for which of the following pts. would autologous donation NOT be advisable? a-patients with an alloantibody against a high-incidence antigen b- patients with uncompensated anemia c- open heart surgery patients d- patients with multiple antibodies

B- patients with uncompensated anemia

component of choice for transfusion therapy for - patients who are thrombocytopenic secondary to the treatment of acute leukemia a- RBC b- platelet concentrate c- whole blood d- cryoprecipitate e- transfusion not indicated f- leuko- reduced

B- platelet concentrate

which of the following statements is NOT true of anti-Fya and anti-Fyb? a- are clinically significant b- react well with enzyme treated panel cells c- cause HTR d- cause a generally mild hemolytic disease of the newborn

B- react well with enzyme treated panel cells. ( enzymes denature Fya and Fyb antigens and render the panel Fy(a-b-)

when 2,3-BPG levels drop in stored blood, which of the following occurs as a result? a- red blood cell K increases b- red blood cell ability to release O2 decreases c- plasma Hgb is stabilized d- ATP synthesis increases

B- red blood cell ability to release O2 decreases

a pooled sera product from 16 donors has a repeatedly positive nucleic acid test for HCV. the next action that should be taken is to a- permanently exclude all the donors in the pool b- test each donor in the pool for HCV c- label all the donors positive for HCV d- confirm the positive using recombinant immunoblot assay (RIBA)

B- test each donor in the pool for HCV

Husband: D-C+ E-c+e+; weak D + Wife: D-C-E-c+e+; weak D - Infant: D+C-E-c+e+; N/A which, if any, of these three individuals can make anti-D? a- Husband b- Wife C-Husband and Wife d infant

B- wife (husband has weak D due to position affect of the C gene being in a trans position to the normal D gene, often causing a weakened expression of the D Ag)

which of the following antibodies can be neutralized with pooled human plasma? a- Anti-Hy and anti-Ge:1 b- anti-Cha and anti- Rga c- anti-Coa and anti-Cob d- anti-Doa and anti- Jsb

B-anti-Cha and anti-Rga ( these antigens are actually pieces of complement component 4 (C4) and are present on some RBCs, although not an antigen. IgG antibodies that show a 1+ agglutination on IAT but typically not clinically significant)

the antiglobulin test does not require washing or the addition of IgG-coated cells in which of the following antibody detection meethods? a- Solid phase red cell adherence assays b- gel test c- affinity column assay d- PEG technique (similar to LISS)

B-gel test (read book for further useful info on these third generation technologies)

a patient had an anti-E identified on his serum 5 years ago. his antibody screening is now negative. to obtain suitable blood for transfusion, what is the best procedure? a- type the patient for the E antigen as an added part to the crossmatch procedure b- type the donor units for the E antigen and crossmatch the E-negative units c- crossmatch donors with the patients serum and release the compatible units for transfusion d- perform the crossmatch with enzyme treated donor cells, because enzyme treated RBC cells react better ith Rh antibodies

B-type the donor units for the E antigen and crossmatch the E-neg units

shelf life of FFP prepared from whole blood a- 10 years b- 2 years c- 1 year d- 35 days

C- 1 year

the temperature range for maintaining red blood cells and whole blood during shipping is a- 0-4C b- 1-6C c- 1-10C d- -15C

C- 1-10C

shelf life of RBC in CPDA-1 prepared from whole blood a- 42 days b- 21 days c- 35 days d- 5 days

C- 35 days

A satellite bag containing 250mL of fresh plasma is selected for quality control of cryoprecipitate production. cryprecipitate is prepared according to standard operating procedures. the final product has a total volume of 10mL. the factor VIII assays are 1UL/mL before and 9IU/mL after preparation what is the percent yield of factor VIII in the final cryoprecipitate? a- 11% b-25% c- 36% d- 80%

C- 36% (90UI x 10mL)/ (1IU x 250mL)= 0.36

a 70kg man has a plt count of 15,000/uL and there are no complicating factors such as fever or HLA sensitization. if he is given plt pool of 6units, what would you expect his posttransfusion count to be? a-21,000-27,000/uL b- 25,000-35,000/uL c- 45,000-75,000/uL d- 75,000-125,000/uL

C- 45,000-75,000/uL (increase in 5,000-10,000/uL per unit plt. thus: ( 6 x 5000) + 15,0000 = 45000 and (6 x 10) + 15000 = 75000)

A potential donor has no exclusions, but she weighs only 95lbs. what is the allowable amount of blood that can be drawn? a- 367 mL b- 378 mL c- 454 mL d- 473 mL

C- 454 mL allowed to donate no more than 10.5mL/kg of body donor body weight. thus--> 95/2.2lb per kg=43.2kg-->43.2 x 10.5mL=453.6mL of blood

platelets play an important role in maintaining hemostasis. one unit of donor platelets derived form whole blood should yield ___________ platelets a- 5.5x10^6 b 5x10^8 c- 5.5x10^10 d- 5x 10^10

C- 5.5x10^10

Husband: D-C+E-c+e+; weak D + Wife: D-C-E-c+e+; weak D - Infant: D+C-E-c+e+; N/A what % of this couples offspring can be expected to be D-neg a-0% b-25% c-50% d-75%

C- 50% (perform punnet square)

often when trying to identify a mixture of antibodies, it is useful to neutralize one of the known antibodies. which one of the following antibodies is neutralizable? a- Anti-D b- Anti-Jka c-Anti-Lea d- Anti-M

C- Anti-Lea

a black patient has the following Rh phenotype: D+ C+ E+ c+ e+. which of the following genotypes is LEAST probable? a- DCE/dce b-DCe/DcE c- DCe/dcE d- DcE/dCe

C- DCe/dcE---->all of these genotypes have low freq. in the black pop'n. DCe/dcE is the rarest, with a freq. of <0.1%. DCe/DcE has the highest freq. of 3.7%

a victim of an auto accident arrives in the ER as a transfer from a hospital in a rural area. the patient has been in that facility for several weeks and has received several units of RBC during that time. the ER resident orders 2 units of RBC for transfusion. the sample sent to the BB is centrifuged and the cell-serum interface is not discernable. a subsequent sample produces the same appearance. you would suspect that the patient has a- autoimmune hemolytic anemia b- anti-FYa c-anti-JKa d- paraxysmal nocturnal hemoglobinuria

C- anti-JKa (often declines in serum to below detectable levels. if antibody made in response to JKa exposure and not transfused again for a while, it might not show up on the antibody screen. An intravascular delayed transfusion reaction is characteristic for Kidd antibodies because, after second stimulation, there is a slow rise in antibody titer and they activate complement very well.)

which of the following antibodies does NOT match the others in terms of optimal reactive temp? a- anti-Fya b- anti-Jkb c-anti-N d-anti-U

C- anti-N (this is the only one listed that is generally a RT saline agglutinin. the others listed ar best detected using IAT phase)

A woman wants to donate blood. her physical examination reveals the following: weight-110lbs, pulse-73 bpm, BP-125/75mm hg, Hct-35%. which of the following exclusions applies to the prospective donor? a- pulse to high b- weight to low c- hematocrit to low d blood pressure to high

C- hematocrit to low, needs to be >= 36%

the pH of four platelet concentrates is measured on the day of expiration. the pH and plasma vol. of four of the units are as follows: pH 6, 5mL; pH 5.5, 38mL; pH 5.8, 40mL; pH 5.7, 41mL. what corrective action is needed in product preparation to meet AABB standards for platelet production? a- no corrective action b- recalibrate pH meter c- increase final plasma vol. of plt concentrate d- decrease final plasma vol. of plt concentrate

C- increase final plasma vol. of plt concentrate (should not fall below pH 6.2)

a patient arrives in the OB clinic 3mths pregnant. this is her first pregnancy and has never been transfused. her prenatal screen includes the following: ABO- 0 SC 1- IS/37/AHG 4+ SC II- IS/37/AHG 4+ AC- 0 check cells- pass these test results could be due to: a- cold autoantibody b- inheritance of sese genes c- inheritance of hh genes d- rouleaux

C- inheritance of hh genes (bombay and has anti-H antibodies. cold auto ruled out by AC, sese genes do not effect RBC or Rh typing, and rouleaux ruled out by AHG phase still being positive)

a donor indicates that he has taken two aspirin tablets per day for the 36hrs. the unit of blood a- may not be used for pooled platelet concentrate preparation b- should not be drawn till 36hrs after cessation of aspirin ingestion c- may be used for pooled platelet concentrate preparation d- may be used for red blood cells and fresh-frozen plasma production, but the platelets should be discarded

C- may be used for pooled platelet concentrate preparation

during preparation of plt concentrate, the hermetic seal of the primary bag is broken. the red blood cells a- must be discarded b- may be labeled with 21- day expiration date of collected in CPD c- must be labeled with a 24hr expiration date d- may b glycerolized within 6days and store frozen

C- must be labeled with 24hr expiration date of when hermetic seal was broken

it has been determined that a patient has posttransfushion hepatitis and recieved blood from eight donors. there is nothing to indicate that these donors may have been likely to transmit hepatitis. what action must be taken initially? a- defer all donors indefinitely from further donations b- repeat all hepatitis testing on a fresh sample from each donor c- notify the donor center that collected the blood d- interview all implicated donors

C- notify the donor center that collected the blood

which of the following is least likely to transmit hepatitis? a- cryoprecipitate b- RBC c- plasma protein fraction d- platelets

C- plasma protein fraction ( A standard sterile preparation of serum albumin and globulin obtained by fractionating blood, serum, or plasma from healthy human donors and testing for absence of hepatitis B surface antigen. It is used as a blood volume expander.)

which of the following best describes what must be done with a unit of blood drawn from a donor who is found to be at high risk off contracting acquired immune deficiency syndrome (AIDS)? a- hold unit in quarantine until donor diagnosis is verified b- use blood for research dealing with AIDS c- properly dispose of unit by autoclaving or incineration d- use plasma and destroy red blood cells

C- properly dispose of unit by autoclaving or incineration

the pilot tubes for donor unit #3276 break in the centrifuge. you should a- label the blood using the donors previous record b- discard the unit because processing procedures cannot be performed c- remove sufficient segments to complete donor processing procedures

C- remove sufficient segments to complete donor processing procedures

component of choice for transfusion therapy for - patients with normovolemic? a- RBC b- platelet concentrate c- whole blood d- cryoprecipitate e- transfusion not indicated f- leuko- reduced

C- whole blood (ANH is a technique in which whole blood is removed from a patient, while circulating volume is maintained with acellular fluid. It is performed shortly before or shortly after induction of anaesthesia. ANH is a technique that may be considered in patients undergoing surgery in which substantial blood loss is anticipated. )

blood needs to be prepared for intrauterine transfusion of the fetus with severe HDN. the red cell unit selected is compatible with the mothers serum and has been leuko-depleted. an additional step that must be take before transfusion is to? a- add pooled platelets and fresh frozen plasma b- check that the RBC group is consistent with the fathers c- irradiate the RBC before infusion d- test the RBC unit with the neonates eluate

C-irradiate the rbc before infusion

Husband: D-C+ E-c+e+; weak D + Wife: D-C-E-c+e+; weak D - Infant: D+C-E-c+e+; N/A which of the following conclusions regarding the family typing is most likely? a- the husband is not the infants father b- the husband is proved to be the father c- the husband cannot be excluded from being the father d- the D typing on the infant is a false positive

C-the husband cannot be excluded from being the father (bc of his weak D phenotype)

a centrifuge used for platelet preparation has been returned after a major repair. a unit of whole blood(450mL; platelet count 200,000/uL) is selected for calibration of platelet production. the PRP contains 250mL with a plt count of 300,000/uL. the final plt concentrate prepared from PRP contains 50mL with a plt count of 900,000/uL what is the % yield of plts in the PRP from this unit? a- 33% b- 45% c- 66% d- 83%

D- 83% (300,000 x 250 (= 75,000,000) / 200,000 x 450 (=90,000,000) = 0.83 x 100= 83%)

which of the following tests is/are not performed during processing? a-ABO and Rh grouping b- HBsAg c- HIV-1-Ag d- HBsAb

D- HBsAb (HBV DNA and HBsAg are the first viral markers to circulate in an individual infected with HBV. Anti-HBc appears in the blood of individuals infected with HBV one to four weeks after the appearance of HBsAg, and at the onset of symptoms for the minority of individuals (5% or less) who develop symptoms)

most blood group antibodies are of what immunoglobulin classes? a- IgA and IgD b-IgA and IgM c- IgE and IgD d- IgG and IgM

D- IgG and IgM

which of the following sugars must be present on a precursor substance for A and B antigenic activity to be expressed? a- D-Galactose b- N-Acetylgalactosamine c- Glucose d-L-Fructose

D- L-fructose

which of the following weak D donor units should be labeled Rh- positive? a-weak D due to transmissible genes b- weak D due as position effect c- weak partial D d- all the above

D- all of the above

although cryoprecipitate has primarily been used to treat hypofibringenemia and hemophilia A, it contains other blood proteins useful in the treatment of coagulopathies. which of the following is NOT found in cryoprecipitate? a- fribronectin b- factor XIII c- factor VIII:vW d- antithrombin III

D- antithrombin III

a recent transfused patients serum has a positive antibody screen. the panel performed at IS, in LISS at 37C, and at AHG shows a strong anti-Fya and a weak possible anti-C. to confirm the anti-C, you should perform... a- elution b- absorption c- antigen typing d- enzyme panel

D- enzyme panel ( Fya is destroyed by enzyme treatment--therefore the anti-Fya seen in the initial panel will not react in an enzyme-treated panel. enzyme treated cells react well with antibodies of the Rh system, thus if anti-C is present, it will show up on the enzyme panel)

which of the following blood groups reacts LEAST strongly with anti-H produced in group in A1B individual? a- Group O b-Group A2B c- Group A2 d- Group A1

D- group A1 (order of decreasing reactivity with anti-H is: O ,A2 ,A2B ,A1 ,A1B)exception would be rare bombay individual becuase they lack the H anitgen. group O has the most H antigens whereas group A1B cella contain the least H antigen.

which of the following conditions would contraindicate autologous presurgical donation? a- weight of 100lbs b- age of 14yrs old c- hemoglobin of 12g/dL d- mild bacteremia

D- mild bacteremia

the blood bank procedures manual must be a- revised annually b- revised after publication of each new edition of AABB standards c- reviewed prior to scheduled inspection d- reviewed annually by an authorized individual

D- must be annually reviewed by authorized individual

which of the following statements is NOT true of anti-U? a- is clinically sig. b- is only found in black individuals c- only occurs in S-s- individuals d- only occurs in Fy(a-b-) individuals

D- only occurs in Fy(a-b-). (causes HTR and HDN; all white people appear to be U+ because no neg have been found; 99% black pop'n U-; those that are U- are also S-s-). Fya has nothing to do with U antigen.

Even though it is properly collected and stored, which of the following will fresh-frozen plasma NOT provide a- factor V b- factor VIII c- factor IX d- platelets

D- platelets

which of the following donors would be differed indefinitely? a- history of syphilis b- history of gonorrhea c- acutane treatment d- recipient of human growth horomone

D- recipient of human growth hormone (causes increase in RBC indices)

testing need to be done with anti-serum that is rarely used. the appropriate steps to take in using this antiserum include following manufactures procedure and a- performing a cell panel to be sure that the antiserum is performing correctly b- performing the testing on screen cells c- testing in duplicate to ensure the repeatability of the results d- testing a cell that is negative for the antigen and one that is heterozygous for the antigen.

D- testing a cell that is negative for the antigen and one that s heterozygous for the antigen ( want to have a negative and positive control-the heterozygous cell is used to determine whether or not the antisera will react with the smaller number of antigen sites on the RBCs seen in heterozygotes, thus serving a positive control)

the following results were obtained when typing a patients blood type: Anti-A: 4+ Anti-B: 2+ A1: 0 B: 4+ the tech suspects that this is a case of an acquired B antigen. which of the following would support this suggestion? a- A positive AC b- secretor studies shows that the patient is a non-secretor c- a patient diagnosis of leukemia d- the patients RBC give a negative result with a monoclonal anti-B reagent lacking the ES-4clone

D- the patients RBC give a negative result with a monoclonal anti-B reagent lacking the ES-4clone

which set of antibodies could you possible find in a patient with no history of transfusion or pregnancy? a- anti-I, anti-s, anti-P1 b- anti Leb, anti-A1, anti-D c- anti-M, anti-c, anti-B d- anti-P1, anti-Lea, anti-I

D-anti-P1, anti-Lea, anti-I (these antibodies are often non-red cell stimulated IgM antibodies, but can also be stimulated by exposure to red cells with corresponding antigens. each of the others listed have at least one antigen that needs to be stimulated upon exposure)

which of the following is characteristic of Kidd system antibodies? a- usually IgM antibodies b- corresponding antigens are destroyed by enzymes c- usually strong and stable storage d- often implicated in DHTR

D-often implicated in DHTR

component of choice for transfusion therapy for -patients with warm autoimmune hemolytic anemia? a- RBC b- platelet concentrate c- whole blood d- cryoprecipitate e- transfusion not indicated f- leuko- reduced

E- transfusion not indicated

component of choice for transfusion therapy for -patient requires transfusion with RBC that will not transmit CMV? a- RBC b- platelet concentrate c- whole blood d- cryoprecipitate e- transfusion not indicated f- leuko- reduced

F- leukocyte reduced RBC

for allogeneic transfusion, should this person be accepted or deferred? - a 35 year old runner (pulse 46 bpm)

accept

for allogeneic transfusion, should this person be accepted or deferred? -a 25 yr old who said he had jaundice right after he was born

accept

for allogeneic transfusion, should this person be accepted or deferred? -a women who had a baby 2mths ago

accept

for allogeneic transfusion, should this person be accepted or deferred? -an 18 yr old with poison ivy on his hands and feet

accept

for allogeneic transfusion, should this person be accepted or deferred? - A 65 year old man whose birthday is tomorrow

accepted

for allogeneic transfusion, should this person be accepted or deferred? -a 50yr old man whom has had sex with another man in 1980

defer indefinitely--any man whom has sex with another man after 1977 must be indefinitely deferred due to possible transmission of HIV virus

allowable shelf life of blood for transfusion therapy with EDTA

not an approved anticoagulant

for allogeneic transfusion, should this person be accepted or deferred? - a 45 year old who donated a unit during a holiday appeal 54 days ago

temporary deferral----time interval between donations is 8 weeks or 56 days to protect health of donor