CD Markers

CD19 will positive stain these normal tissues

CD19 will stain normal: 1. Pre-B, B cells 2. Follicular dendritic cells 3. Hematogones via flow cytometry

CD1a is used to diagnose what dz

CD1a is used to dx *Langerhans cell histiocytosis*

CD21 is the receptor for these viruses and a marker for these human cells

CD21 = receptor for EBV & HHV8 Marker for Dendritic cells

Apical staining of CD10 can dx these dz

*Apical* CD10 stains well-differentiated carcinoma of colon, pancreas & prostate

CD23 is a marker for what cell and acts as a activating factor for

*CD23* (w/ CD21 & 35) is a *Dendritic cell* marker CD23 also is needed to convert B cells to Plasma cells

CD1 binds to what kind of antigen, on which chromosome is it found

CD1 recognized *lipid* antigens is found on various APCs. Found on 1q22-23

CD10 positively stains these normal tissues

CD10 stains normal 1. Pre-B cells 2. Pre-T cells and some T cells 3. germinal center cells 4. Granulocytes Adrenal Cortex, brain, choroid plexus, endometrial stroma, endothelial cells, fibroblasts Male epithelium/hepatocytes/kidney microvilli, Breast myoepithelial cells Ovary, placenta, prostate basal/secretory cells, small intestine, wolffian type epithelium

CD13 is used to dx what dz

CD13 is used 1. AML (13+ 11b+) vs. transient myeloproliferative disorder (13- 11b-) 2. AML (13+) vs ALL (13-)

CD13 stains these normal tissues

CD13 stains normal 1. Granulocytes 2. Macrophages, masts, monocytes, myelomonocytes & osteoclasts 3. Bild duct canaliculi, CNS synapses, endothelial cells, endometrial stromal cells. 4. Fibroblasts, liver, prostate secretory cells, placenta, renal prox. tubules 5. Respiratory epithelium, small intestine & sperm

CD14 stains these normal tissues

CD14 stains these normal tissues 1. *Macrophages/monocytes* 2. Langerhan's cells 3. Dendritic cells 4. Weakly B cells and granulocytes

CD15 is used to dx what dz

CD15 is used to ddx 1. Hodgkin Lymphoma (either membranous/cytoplasmic or golgi staining of Reed-Sternberg cells) - confirms it CD15+ 2. Hodgkin (CD15+) vs. anaplastic large cell lymphoma (CD15- usually)

CD15 stains these normal tissues

CD15 stains these normal 1. Myeloid cells 2. Eosinophils 3. Activated B or T cells (inc. Mono) 4. Prox. tubule 5. Small intestine Paneth cells

CD16 will positive stain these normal tissues

CD16 (Fc part of IgG) stains these nml tissues: 1. NK cells 2. Granulocytes 3. Monocytes/Macrophages 4. Eosinophils 5. Reactive T cells 6. Immature thymocytes 7. Placental trophoblast

CD19 will NOT stain

CD19 will NOT stain: 1. Plasma cells, basophils & mast cells 2. Myeloma 3. Most T cell lymphomas

CD21 will positive stain these normal tissues

CD21 will stain normal: 1. Mature B cells (*esp. marginal and mantle cells*) 2. Follicular dendritic cells 3. pharyngeal & cervical epithelial cells 4. some thymocytes & T cells

CD22 is used to dx

CD22 is a pan B cell marker that is helpful for the dx of *hairy cell leukemia* and also Pre-B ALL. (CD22+)

CD23 is used for what dz

CD23 1. SLL/CLL (CD23+) vs. Mantle cell lymphoma or MALT lymphoma (CD23-) 2. Nodal marginal zone lymphoma vs. follicular lymphoma

CD23 will positive stain these normal tissues

CD23 will stain normal 1. Activated mature B cells w/ IgM or IgD 2. Mantle cells 3. Activated monocytes/macrophages 4. Platelets, eosinophils, Langerhans 5. Follicular dendritic cells 6. Intestinal epithelium

CD24 will positive stain these normal tissues

CD24+ in normal: 1. All B cells & granulocytes 2. Apocrine glands 3. epithelial cells 4. Fallopian tube 5. Kidney cells 6. Muscle 7. Nucleus Pulposis

CD25 is a marker for these 2 diseases

CD25 is a marker for *hairy cell leukemia* and *systemic mastocytosis*

CD25 positive stains these normal tissues

CD25+ in normal: 1. Activated B & T cells 2. Macrophages 3. Some thymocytes, myeloid precursors & some oligodendocytes 4. basophils dim stain on flow

CD25 negative stain help to dx

CD25- 1. Non-neoplastic mast cells 2. Marginal zone B cell lymphoma 3. T cell large granular leukemia/lymphoma 4. usually variant of hairy cell leukemia

What normal tissue express CD3

CD3 1.Thymocytes 2. Peripheral T cell (1st cytoplasmic -> membranous) 3. NK cells (CDe majority cytoplasmic) 4. Purkinje cells of cerebellum

What is the most specific CD marker for T cells

CD3 - used to identify benign/malignant dz

CD30 is used to confirm the dx of; what is the staining pattern?

CD30 used to definitively diagnose 1. anaplastic large cell lymphoma 2. classic Hodgkin's lymphoma 3. embryonal carcinoma golgi & membranous staining pattern

CD30 will stain these normal tissues

CD30+ normal tissues are: 1. Granulocytes 2. Plasma cells 3. Activated B-, T- and NK cells 4. Monocytes 5. decidua

CD31 will stain these normal tissues

CD31 will stain these normal tissues 1. Endothelial cells at cell junctions, including alveolar wall capillaries, glomeruli, sinuses in liver, lymphatics, spleen, megakaryocytes & platelets 2. basophils, granulocytes, Kupffer cells, macrophages, monocytes, NK cells, osteoclasts, plasma cells 3. Fibroblasts, spermatozoa, trophoblast

CD31 is used to diagnose

CD31+ membranous stains dz = 1. Endothelial marker on small/large vessels 2. Confirms vascular origin of tumor (do NOT confuse w/ CD31+ macrophages) and/or vascular invasion

CD33 is used to diagnose these dz

CD33 distinguishes myeloid CD33+ vs Lymphoid leukemias (CD33-) AML M0, AML M1-M5 (75-85%), AML M7 (variable) ALK+ anaplastic large cell lymphoma CML, CMML Burkitt's lymphoma, SLL/CLL or T-ALL

CD33 will stain these normal tissues

CD33+ normal tissues: 1. Myeloid progenitors (decreasing with maturation) 2. Basophils, granulocytes (low), Mast cells, Monocytes, NK cells 3. Plasmacytoid dendritic cells & T cells (some) 4. Identifies monocytes on flow CD33 should NOT be expressed outside the hematopoietic system

CD34 will stain these normal tissues

CD34+ normal tissues: 1. Endothelium of blood vessels (including alveolar & glomuruli) but NOT hepatic/splenic sinusoids 2. Hematopoietic progenitors/hematogones 3. Dendritic interstitial cells, dermal dendrocytes, endometrial stroma, endoneurium, fibrocytes, follicle cells, interstitial cells of Cajal, Mast cells, megakaryocytes, neural stem cells, osteoblasts, perivascular stroma

CD35 is used to diagnose:

CD35+ dx 1. Follicular dendritic cell sarcoma 2. Highligt or confirm lack of dendritic cell germinal center meshwork. (i.e in splenic B cell lymphoma)

CD35 will stain these normal tissues

CD35+ normal tissues: 1. Follicular dendritic cells, erythrocytes 2. Baso-, eosinophils, granulocytes, monocytes, B cells, T cells (10%) & NK cell subset.

CD36+ disease =

CD36+ 1. Alzheimers (microglia, endothelial cells) 2. AML M4-M7 3. Cervical LSIL 4. CML (60%) 5. Gaucher cells, myxoid liposarcoma 6. plasmacytoid T cell lymphoma

CD38 is used to dx these dz

CD38+ 1. identifies plasma cells 2. Dx of Myeloma 3. CLL

What normal tissues express CD4

CD4 - 12p13.31 1. T-helper 2. Thymocytes 3. granulocytes 4. macrophages 5. Langerhans & dendritic cells

What normal tissues express CD5

CD5 1. Thymocytes 2. Almost all T cells 3. B cells in marrow, fetal spleen/cord blood, in peritoneal/pleural cavities and some in mantle zone

CD5 is used as a marker for what diseases?

CD5 marker used for dz ddx 1. Chronic Lymphocytic Leukemia 2. Mantle cell lymphoma 3. nml+malignant T cells 4. Thymic carcinoma

CD7 is used as a marker for what dz

CD7 is a marker for 1. T-Acute lymphocytic leukemia 2. Classical Hodgkin, RS cells neg 3. Cholangiosarcoma 4. Epitheloid sarcoma 5. Pancreatic ductal carcinoma

CD7 stains what normal tissue?

CD7 stains 1. Thymocytes 2. Mature T cell 3. NK cells 4. Monocytes, early myeloid and pre-B cells

CD8 is used as a marker for what dz

CD8 is a marker for 1. Nml and malig T cells 2. Cytotoxic/suppressor T cells 3. Splenic hamartoma (vs, hemangioma or littoral cell angioma CD8 neg)

CD8 positively stains what normal tissues

CD8 stains 1. Cortical Thymocytes 2. CD8 T cells 3. NK cells 4. Dendritic cells

CD9 positively stains what dz

CD9 in dz 1. Pre-B ALL 2. APML 3. Astrocytoma 4. Mast cell disease 5. Angiosarcoma, juvenile nasopharyngeal angiofibroma, kaposi's sarcoma

CD9 positively stains what normal tissues

CD9 stains 1. Pre-B cells 2. Activated T cells 3. Basophils, eosinophils 4. macrophages 5. Plasma cell precursors Brain, cardiac mm, GI system, Kidney, liver, lymphatic epithelium, ovarian surface epithelium, peripheral nerves, skin, spleen, thyroid, tonsil

A cytoplasmic or membranous staining of CD10 can dx these dz

Cytoplasmic/membranous CD10 1. Poorly-diff adenocarcininoma 2. Endometrial stromal sarcoma 3. Melanoma 4. Renal cell carcinoma 5. urothelial carcinoma

High expression of CD24 is associated with a poor prognosis in these carcinomas

High CD24 a/w poor prognosis of these carcinomas 1. Bile duct 2. Bladder 3. Breast (w/ CD44) 4. Colorectal 5. Gallbladder 6. Liver 7. Non-small cell lung CA 8. Ovary 9. Pancreas 10. Glioma 11. Osteosarcoma