Chapter 11 - 12 Homework- DNA Profiling✅✅

negative ... positive

In gel electrophoresis DNA molecules migrate from _____ to _____ ends of the gel.

the profile from suspect 2 matches the crime scene

In this Gell, the DNA from the crime scene is loaded into the left-most lane. The DNA from suspect 1 is loaded into the middle lane. And DNA from suspect 2 is loaded in the right lane. What can you conclude from these DNA profiles?

6,557

In this example the marker DNA includes fragments that have 23,130, 9,416, 6,557, 4,361, 2,322, 2,027, and 564 base pairs. Approximately how many base pairs are in the DNA fragment indicated by the letter A?

Suspect 2

Which of the following suspects matches the samples taken from the crime scene?

gastrin and GH1

Which of these genes are located on the q arm of chromosome 17?

GH1

Which of these genes codes for a protein that plays a role in growth?

MPO

Which of these genes codes for a protein that plays a role in white blood cell function?

q arm

The gene that codes for gastrin is located on the _____ of chromosome 17.

1,268

DNA fragment A consists of _____ base pairs.

1,405

DNA fragment B consists of _____ base pairs.

that the DNA polymerase could be denatured

DNA polymerase is a heat-sensitive enzyme. What is one thing that would need to be considered concerning the activity of this enzyme in PCR when the temperature is heated during each cycle to separate the DNA strands?

the DNA will move up the gel

I've accidentally hooked up the wires to this gel electrophoresis chamber backwards, with the positive pole at the top and the negative pole at the bottom. If I turn on the power with it hooked up this way, what will happen to the DNA?

A

If I cut a piece of DNA into one long and two short hours pieces, which of the following gels shows how it would look?

heat

In a PCR reaction, the strands of DNA are first separated by ___.

between 6,557 and 4,361 base pairs

In this example the marker DNA includes fragments that have 23,130, 9,416, 6,557, 4,361, 2,322, 2,027, and 564 base pairs. Approximately how many base pairs are in the DNA fragment indicated by the letter B?

involved in eye development

The RP13 gene of chromosome 17 codes for a protein _____.

involved in the regulation of the cell cycle

The TP53 gene of chromosome 17 codes for a protein _____.

PCR

This machine is used to create a large sample of DNA for testing from a small sample of DNA. What is that method called?

separation of DNA strands; addition of primers; use of DNA polymerase to produce second strand of DNA

What is the correct sequence of events that occur in a PCR reaction?

phosphate

What of a DNA nucleotide contains a negative charge?

When there are small quantities of DNA to analyze

When is PCR particularly applicable?