Enterobacteriaceae

Care must be taken when identifying biochemical isolates of Shigella because serological cross reactions occur with: A. E. coli B. Salmonella spp. C. Pseudomonas spp. D. Proteus spp.

A. E. coli

Infections caused by Yersinia pestis are rare in the United States. Those cases that do occur are most frequently located in which region? A. New Mexico, Arizona, and California B. Alaska, Oregon, and Utah C. North and South Carolina and Virginia D. Ohio, Michigan, and Indiana

A. New Mexico, Arizona, and California

Three blood cultures taken from a 30-year-old cancer patient receiving chemotherapy and admitted with a urinary tract infection grew lactose-negative, motile, gram-negative rods prior to antibiotic therapy. Given the following biochemical reactions, which is the most likely organism? H2S (TSI) = + VP = Neg DNase = + Gelatin hydrolysis = + Ornithine decarboxylase = Neg Indole = Neg Citrate = Neg Phenylalanine deaminase = + MR = + Urease = + A. Proteus vulgaris B. Proteus mirabilis C. Serratia marcescens D. Klebsiella pneumoniae

A. Proteus vulgaris

Biochemically, the Enterobacteriaceae are gram-negative rods that: A. Ferment glucose, reduce nitrate to nitrite, and are oxidase negative B. Ferment glucose, produce indophenol oxidase, and form gas C. Ferment lactose and reduce nitrite to nitrogen gas D. Ferment lactose and produce indophenol oxidase

A. Ferment glucose, reduce nitrate to nitrite, and are oxidase negative

WhichgeneraoftheEnterobacteriaceaeareknownto cause diarrhea and are considered enteric pathogens? A. Enterobacter, Klebsiella, Providencia, and Proteus B. Escherichia, Salmonella, Shigella, and Yersinia C. Pseudomonas, Moraxella, Acinetobacter, and Aeromonas D. Enterobacter, Citrobacter, and Morganella

B. Escherichia, Salmonella, Shigella, and Yersinia

Which group of tests best differentiates Enterobacter aerogenes from Edwardsiella tarda? A. Motility, citrate, and urease B. Hydrogen sulfide (H2S) production, sucrose fermentation, indole, and VP C. Lysine decarboxylase, urease, and arginine dihydrolase D. Motility, H2S production, and DNase

B. Hydrogen sulfide (H2S) production, sucrose fermentation, indole, and VP

A gram-negative rod is recovered from a catheterized urine sample from a nursing home patient. The lactose-negative isolate tested positive for indole, urease, ornithine decarboxylase, and phenylalanine deaminase and negative for H2S. The most probable identification is: A. Edwardsiella spp. B. Morganella spp. C. Ewingella spp. D. Shigella spp.

B. Morganella spp.

Which genera are positive for phenylalanine deaminase? A. Enterobacter, Escherichia, and Salmonella B. Morganella, Providencia, and Proteus C. Klebsiella and Enterobacter D. Proteus, Escherichia, and Shigella

B. Morganella, Providencia, and Proteus

The Voges-Proskauer (VP) test detects which end product of glucose fermentation? A. Acetoin B. Nitrite C. Acetic acid D. Hydrogen sulfide

A. Acetoin

The drugs of choice for treatment of infections with Enterobacteriaceae are: A. Aminoglycosides, trimethoprim-sulfamethoxazole, third-generation cephalosporins B. Ampicillin and nalidixic acid C. Streptomycin and isoniazid D. Chloramphenicol, ampicillin, and colistin

A. Aminoglycosides, trimethoprim-sulfamethoxazole, third-generation cephalosporins

In the test for urease production, ammonia reacts to form which product? A. Ammonium citrate B. Ammonium carbonate C. Ammonium oxalate D. Ammonium nitrate

B. Ammonium carbonate

Which of the following organisms is often confused with the Salmonella species biochemically and on plated media? A. E. coli B. Citrobacter freundii C. Enterobacter cloacae D. Shigella dysenteriae

B. Citrobacter freundii

The Shiga-like toxin (verotoxin) is produced mainly by which Enterobacteriaceae? A. Klebsiella pneumoniae B. E. coli C. Salmonella typhimurium D. Enterobacter cloacae

B. E. coli

Which of the following tests best differentiates Shigella species from E. coli? A. Hydrogen sulfide, VP, citrate, and urease B. Lactose, indole, ONPG, and motility C. Hydrogen sulfide, MR, citrate, and urease D. Gas, citrate, and VP

B. Lactose, indole, ONPG, and motility

A 6-year-old female patient was admitted to the hospital following 2 days of severe diarrhea. Cultures from three consecutive stool samples contained blood and mucus. Patient history revealed a hamburger lunch at a fast-food restaurant 3 days earlier. Which pathogen is most likely responsible for the following results? Growth on: XLD agar = yellow colonies HE agar = yellow colonies Mac agar = light pink and dark pink colonies Mac with sorbitol agar - few dark pink and many colorless colonies A. Salmonella spp. B. Shigella spp. C. E. coli O157:H7 D. Yersinia enterocolitica

C. E. coli O157:H7

Which agar that is used for routine stool cultures is the medium of choice for the isolation of Yersinia strains from stool specimens? A. Salmonella-Shigella agar B. Hektoen enteric agar C. MacConkey agar D. CNA agar

C. MacConkey agar

Putrescine is an alkaline amine product of which bacterial enzyme? A. Arginine decarboxylase B. Phenylalanine deaminase C. Ornithine decarboxylase D. Lysine decarboxylase

C. Ornithine decarboxylase

Lysine iron agar (LIA) showing a purple slant and a blackened butt indicates: A. E. coli B. Citrobacter spp. C. Salmonella spp. D. Proteus spp.

C. Salmonella spp.

The malonate test is most useful in differentiating which members of the Enterobacteriaceae? A. Shigella B. Proteus C. Salmonella subgroups 2, 3 (the former Arizona) D. Serratia

C. Salmonella subgroups 2, 3 (the former Arizona)

Kligler iron agar (KIA) differs from triple-sugar iron agar (TSI) in the: A. Ratio of lactose to glucose B. Ability to detect H2S production C. Use of sucrose in the medium D. Color reaction denoting production of acid

C. Use of sucrose in the medium

Three consecutive stool cultures from a 25-year-old male patient produced scant normal fecal flora on MacConkey and Hektoen agars. However, colonies on CIN agar (cefsulodin-irgasan-novobiocin) displayed "bulls-eye" colonies after 48 hours incubation. The patient had been suffering from enterocolitis with fever, diarrhea, and abdominal pain for 2 days. What is the most likely identification of this gram-negative rod? A. E. coli B. Proteus mirabilis C. Yersinia enterocolitica D. Klebsiella pneumoniae

C. Yersinia enterocolitica

At which pH does the methyl red (MR) test become positive? A. 7.0 B. 6.5 C. 6.0 D. 4.5

D. 4.5

Decarboxylation of the amino acids lysine, ornithine, and arginine results in the formation of: A. Ammonia B. Urea C. CO2 D. Amines

D. Amines

Four blood cultures were taken over a 24-hour period from a 20-year-old woman with severe diarrhea. The cultures grew motile (room temperature), gram-negative rods. A urine specimen obtained by catheterization also showed gram-negative rods, 100,000 col/mL. Given the following results, which is the most likely organism? TSI = A/A gas VP = Neg H2S = Neg Urease = Neg Phenylalanine deaminase = Neg Indole = + MR = + Citrate = Neg Lysine decarboxylase = + A. Proteus vulgaris B. Salmonella typhi C. Yersinia enterocolitica D. E. coli

D. E. coli

Which of the following organisms are generally positive for β-galactosidase? A. Salmonella spp. B. Shigella spp. C. Proteus spp. D. E. coli

D. E. coli

Which single test best separates Klebsiella oxytoca from K. pneumoniae? A. Urease B. Sucrose C. Citrate D. Indole

D. Indole

Which group of tests, along with colonial morphology on primary media, aids most in the rapid identification of the Enterobacteriaceae? A. MR and VP, urease, and blood agar plate B. Phenylalanine deaminase, urease, and CDC agar plate C. Bacitracin, β-lactamase, and MacConkey agar plate D. Indole, oxidase, MacConkey, and blood agar plates

D. Indole, oxidase, MacConkey, and blood agar plates

Direct spread of pneumonic plague disease occurs by which route? A. Fecal-oral route B. Rat bite C. Ingestion of contaminated tissue D. Inhalation of contaminated airborne droplets

D. Inhalation of contaminated airborne droplets

A leg culture from a nursing home patient grew gram-negative rods on MacConkey agar as pink to dark pink oxidase-negative colonies. Given the following results, which is the most likely organism? TSI = A/A VP = + Urease = + Indole = Neg Citrate = + Motility = Neg MR = Neg H2S = Neg Antibiotic susceptibility: resistant to carbenicillin and ampicillin A. Serratia marcescens B. Proteus vulgaris C. Enterobacter cloacae D. Klebsiella pneumoniae

D. Klebsiella pneumoniae

Members of the genus Cedecea are best differentiated from Serratia spp. by which test result? A. Positive motility B. Positive urease C. Positive phenylalanine deaminase D. Negative DNase

D. Negative DNase

In the Kauffmann-White schema, the combined antigens used for serological identification of the Salmonella spp. are: A. O antigens B. H antigens C. Vi and H antigens D. O, Vi, and H antigens

D. O, Vi, and H antigens

Which species of Shigella is most commonly associated with diarrheal disease in the United States? A. S. dysenteriae B. S. flexneri C. S. boydii D. S. sonnei

D. S. sonnei

Which isolates of the Enterobacteriaceae family most commonly produce extended-spectrum β-lactamase (ESBL)? A. E. coli and Klebsiella pneumoniae B. Yersinia enterocolitica and Yersinia pestis C. Morganella morganii and Proteus vulgaris D. Salmonella typhi and Shigella sonnei

A. E. coli and Klebsiella pneumoniae

A positive Simmons citrate test is seen as a: A. Blue color in the medium after 24 hours of incubation at 35°C B. Red color in the medium after 18 hours of incubation at 35°C C. Yellow color in the medium after 24 hours of incubation at 35°C D. Green color in the medium after 18 hours of incubation at 35°C

A. Blue color in the medium after 24 hours of incubation at 35°C

Which organism is sometimes mistaken for Salmonella and will agglutinate in Salmonella polyvalent antiserum? A. C. freundii strains B. Proteus mirabilis strains C. S. sonnei strains D. E. coli

A. C. freundii strains

Fever, abdominal cramping, watery stools, and fluid and electrolyte loss preceded by bloody stools 2-3 days before is characteristic of shigellosis but may also result from infection with: A. Campylobacter spp. B. Salmonella spp. C. Proteus spp. D. Yersinia spp.

A. Campylobacter spp.

Which group of tests best identifies the Morganella and Proteus genera? A. Motility, urease, and phenylalanine deaminase B. Malonate, glucose fermentation, and deoxyribonuclease (DNase) C. Indole, oxidase, MR, and VP D. Indole, citrate, and urease

A. Motility, urease, and phenylalanine deaminase

A routine, complete stool culture procedure should include media for the isolation of E. coli O157:H7 as well as: A. Salmonella, Shigella, Yersinia, Campylobacter, and Staphylococcus aureus B. Vibrio cholerae, Brucella, and Yersinia spp. C. S. aureus, group B streptococci, and group D streptococci D. Clostridium difficile, Clostridium perfringens, and Yersinia spp.

A. Salmonella, Shigella, Yersinia, Campylobacter, and Staphylococcus aureus

A bloody stool cultured from a 26-year-old woman after 3 days of severe diarrhea showed the following results at 48 hours after being plated on the following media: MacConkey agar: little normal flora with many non-lactose-fermenting colonies Hektoen enteric agar: many blue-green colonies Campylobacter blood agar and C. difficile agar: no growth Clear colonies (from MacConkey agar) tested negative for oxidase, indole, urease, motility, and H2S The most likely identification is: A. Shigella spp. B. Salmonella spp. C. Proteus spp. D. E. coli

A. Shigella spp.

Which genera of Enterobacteriaceae are usually nonmotile at 36°C? A. Shigella, Klebsiella, and Yersinia B. Escherichia, Edwardsiella, and Enterobacter C. Proteus, Providencia, and Salmonella D. Serratia, Morganella, and Hafnia

A. Shigella, Klebsiella, and Yersinia

An isolate of E. coli recovered from the stool of a patient with severe bloody diarrhea should be tested for which sugar before sending it to a reference laboratory for serotyping? A. Sorbitol (fermentation) B. Mannitol (oxidation) C. Raffinose (fermentation) D. Sucrose (fermentation)

A. Sorbitol (fermentation)

A stool culture from a 30-year-old man suffering from bloody mucoid diarrhea gave the following results on differential enteric media: MacConkey agar = clear colonies; XLD agar = clear colonies; Hektoen agar = green colonies; Salmonella-Shigella agar = small, clear colonies Which tests are most appropriate for identification of this enteric pathogen? A. TSI, motility, indole, urease, Shigella typing with polyvalent sera B. TSI, motility, indole, lysine, Salmonella typing with polyvalent sera C. TSI, indole, MR, VP, citrate D. TSI, indole, MR, and urease

A. TSI, motility, indole, urease, Shigella typing with polyvalent sera

Enterobacter sakazakii can best be differentiated from Enterobacter cloacae by which of the following characteristics? A. Yellow pigmentation and negative sorbitol fermentation B. Pink pigmentation and positive arginine dihydrolase C. Yellow pigmentation and positive urease D. H2S production on TSI

A. Yellow pigmentation and negative sorbitol fermentation

Following a 2-week camping trip to the Southwest (US), a 65-year-old male patient was hospitalized with a high fever and an inflammatory swelling of the axilla and groin lymph nodes. Several blood cultures were obtained, resulting in growth of gram-negative rods resembling "closed safety pins." The organism grew on MacConkey's agar showing non-lactose-fermenting colonies. Testing demonstrated a nonmotile rod that was biochemically inert. What is the most likely identification? A. Yersinia pestis B. Klebsiella pneumoniae C. Proteus vulgaris D. Morganella morganii

A. Yersinia pestis

Which of the following reagents is added to detect the production of indole? A. p-Dimethylaminobenzaldehyde B. Bromcresol purple C. Methyl red D. Cytochrome oxidase

A. p-Dimethylaminobenzaldehyde

The majority of clinical laboratories with a microbiology department should have the capability of serotyping which pathogenic Enterobacteriaceae? A. Yersinia enterocolitica, Shigella spp. B. E. coli O157:H7, Salmonella spp., Shigella spp. C. Yersinia pestis, Salmonella spp. D. Edwardsiella spp., Salmonella spp.

B. E. coli O157:H7, Salmonella spp., Shigella spp.

Which of the following organisms, found in normal fecal flora, may be mistaken biochemically for the genus Yersinia? A. Klebsiella spp. B. Proteus spp. C. E. coli D. Enterobacter spp.

B. Proteus spp.

A leg-wound culture from a hospitalized 70-year-old diabetic man grew motile, lactose-negative colonies on MacConkey agar. Given the following biochemical reactions at 24 hours, what is the most probable organism? H2S (TSI) = Neg MR = Neg DNase = + Urease = Neg Phenylalanine deaminase = Neg Ornithine and lysine decarboxylase = + Arginine decarboxylase = Neg Gelatin hydrolysis = + Indole = Neg VP = + Citrate = + A. Proteus vulgaris B. Serratia marcescens C. Proteus mirabilis D. Enterobacter cloacae

B. Serratia marcescens (Gelatin hydrolysis and DNase are positive for both the Proteus spp. and Serratia, but the negative urease and phenylalanine deaminase are differential.)

The ortho-nitrophenyl-β-galactopyranoside (ONPG) test is most useful when differentiating: A. Salmonella spp. from Pseudomonas spp. B. Shigella spp. from some strains of Escherichia coli C. Klebsiella spp. from Enterobacter spp. D. Proteus vulgaris from Salmonella spp.

B. Shigella spp. from some strains of Escherichia coli

Why might it be necessary for both pink (lactose-positive) and colorless (lactose-negative) colonies from an initial stool culture on MacConkey agar to be subcultured and tested further for possible pathogens? A. Most Shigella strains are lactose positive B. Most Salmonella strains are maltose negative C. Most Proteus spp. are lactose negative D. Pathogenic E. coli can be lactose positive or lactose negative

D. Pathogenic E. coli can be lactose positive or lactose negative

Cold enrichment of feces (incubation at 4°C) in phosphate-buffered saline prior to subculture onto enteric media enhances the recovery of: A. Enterotoxigenic E. coli B. Salmonella paratyphi C. Hafnia alvei D. Y. enterocolitica

D. Y. enterocolitica