Enzyme: Test procedures

Which type of kinetic reactions are reactions in which the enzyme is in excess and the substrate concentration is the limiting factor?

First Order

The recommended storage temperature for the preservation of activity for MOST enzymes is:

4

An electrophoretic separation of lactate dehydrogenase isoenzyme that demonstrates elevation in LD-1 greater than LD-2 could be indicative of:

Hemolysis

When measuring enzyme activity, if the instrument is operating 5ºC lower than the temperature prescribed for the method, how will the results be affected?

Lower than actual

An important metallic activator of alkaline phosphatase and creatine kinase is:

Magnesium

According to the Michaelis-Menton kinetics theory, when a reaction is performed in zero-order kinetics:

The substrate concentration is in excess and the reaction rate is dependent on the enzyme concentration

Mrs. Jones, a diabetic, is admitted to the hospital on November 25th. It appears that she has had an acute myocardial infarction (AMI), but did not feel the severe chest pain because of diabetic neuropathy. She has not felt well since November 20th and her physician believes she possibly had an AMI on November 20th.Assuming that the infarct was uncomplicated, which of the following cardiac biomarkers would most likely still be elevated and would assist in an AMI diagnosis?

Troponin T