hematology analyzers
how do impedance Counters work
based on the passage of electric current across to electrodes count the impedance of flow as cells pass through a vacuum space and are divided by the volume or size of the cell. Leukocytes erythrocytes and platelets can be counted . can be calibrated to count cells with specific sizes four different species
how do you care for hematology analyzers
calibration and quality controls must be done on a regular basis. Quality control log should be kept. Technicians must be familiar with the machine and minor maintenance procedures.
what do hematology analyzer is generate
complete blood count of red blood cells. White blood cells. Platelet counts. Hemoglobin. Red blood cell indices like mean corpuscular volume mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration
what are the advantages of using an automated cell counter
fast results. Complete and accurate results. Reproducibility of results. Reliability. Less labor-intensive for the technician
how do hematology analyzer work
flow cytometry where cells are counted and their sizes measured as they flow past a detection device + are identified by their size
what are the three basic types of hematology analyzers
laser-based analyzers. Quantitative Buffy coat system. Impedance analyzers
what are some abnormalities that will not be detected with quantitative Buffy coat systems
lymphocytopenia and left shift
what are some disadvantages of an automated cell counter
must have quality control. require maintenance. Machines cannot see morphologies like band neutrophils. Toxic neutrophils. Polychromatophilic red blood cells. Red blood cell parasites. Nucleated red blood cells
should hematology analyzer be used in animal practices like we can use chemistry analyzers
no because the volumes of red blood cells and white blood cells are different
what are the disadvantages of impedance counters
they cannot see cellular morphology they need maintenance and more technical errors can occur due to the instruments. hemolysis and lipemia can affect the cell
laser-based analyzers
use a laser beam to detect the size and density of cellular components with the scattering of light showing cytoplasmic granules in nuclei of the cells and dividing them into groups as monocytes lymphocytes granulocytes and red blood cells. Dies can be added to count mature versus immature red blood cells
how do quantitative Buffy coat systems work
uses differential centrifugation and estimates cellular components by measuring the expanded Buffy coat layer and a hematocrit tube. can report hematocrit. Leukocyte estimation. Platelet estimation. Granulocytes. Lymphocytes/monocytes. useful for screening but it's only an estimation
why is it a problem that the instrument identifies cells by their size
when they lice the red blood cells it exposes white blood cells and hemoglobin. Canines have small white blood cells falsely decreasing the count. Felines have larger white blood cells falsely increasing the count. Feline platelets Clump together falsely increasing the total white blood cell count
