Intestinal glucose transport lab

Assuming that Baby Jane's problem is a defective SGLT1 protein, what is the most likely reason that her blood was sodium high when she had diarrhea? a) Excess water loss in the feces and urine were causing a decrease in plasma volume, elevating blood sodium b) Baby Jane has been drinking excess water to compensate for her diarrhea c) Excess sodium was being absorbed by the intestine d) Excess sodium was being absorbed by the kidney

a) Excess water loss in the feces and urine were causing a decrease in plasma volume, elevating blood sodium

If so, removal of what from her diet should reduce or stop her diarrhea? a) Glucose b) Milk c) Protein d) Potassium

a) Glucose

Was your hypothesis that if a sodium-glucose transporter is driving active absorption of glucose from the gut lumen into the body in the intestine, then it should be located on the serosal side correct? a) No b) Yes c) There is not enough data d) Maybe

a) No

Which of these findings using the everted sac preparation would support the idea that active glucose transport is driven by a Na+/K+ ATPase on the serosal side? a) Ouabain in the serosal solution blocks or reduces glucose concentration on the serosal side b) Ouabain in the mucosal solution blocks or reduces glucose concentration on the mucosal side c) Quabain in the serosal solution blocks or reduces glucose concentration on the mucosal side. d) Ouabain in the mucosal solution blocks or reduces glucose concentration on the serosal side

a) Ouabain in the serosal solution blocks or reduces glucose concentration on the serosal side

If glucose has been actively transported by the intestine from mucosal to serosal side, then: a) The concentration of glucose in the mucosal side solution should be higher b) The concentration of glucose in the serosal side solution should be higher c) Glucose concentrations should be equal in both solutions, but lower than at the start of the experiment d) Glucose concentrations should be equal in both solutions, but higher than at the start of the experiment

b) The concentration of glucose in the serosal side solution should be higher

These data show that: a) A Na+/glucose is not an important component of active glucose transport by the intestine b) A Na+/glucose co-transporter on the serosal side is an important component of active glucose transport by the intestine c) A Na+/glucose is on both sides of intestine d) A Na+/glucose co-transporter on the mucosal side is an important component of active glucose transport by the intestine

d) A Na+/glucose co-transporter on the mucosal side is an important component of active glucose transport by the intestine

Why do we need to create a calibration curve for the spectrophotometer before we test our samples from the intestine? a) We need to make sure all the enzymatic reactions have stopped b) Without this step we can't measure the absorbance of the colored product c) Calibration is only done for quality control in a lab, it is not really necessary d) It will allow us to convert absorbance values to a more meaningful unit like concentration

d) It will allow us to convert absorbance values to a more meaningful unit like concentration

Why does ORT therapy work? a) ORT cures cholera b) ORT ensures that the person's blood glucose levels stay high enough c) ORT prevents the SGLT activity d) ORT stimulates Na+, glucose and water absorption by the intestine, replacing fluid lost by diarrhea

d) ORT stimulates Na+, glucose and water absorption by the intestine, replacing fluid lost by diarrhea

There was 10 mmol/L glucose on the mucosal side and 45.6 mmol/L on the serosal side. So, was there active transport of glucose from mucosal to serosal side of the jejunum? a) Active transport went from the serosal to the mucosal side b) Can't tell c) No d) Yes

d) Yes

What do these results suggest? intestine a) A Na+/K+ ATPase on the mucosal side is important in active glucose transport by the intestine b) A Na+/K+ ATPase on the serosal side is important in glucose active transport by the c) Blocking the Na+/K+ ATPase reverses glucose transport by the intestine d) The Na+/K+ ATPase is not involved in glucose transport by the intestine

intestine b) A Na+/K+ ATPase on the serosal side is important in glucose active transport by the