JP Lab6A-5: OF Test
What component of this media reduces O2 diffusion? A) mineral oil overlay B) thioglycollate C) carbohydrates D) cystiene
A
Why does amino acid consumption only occur in the "uncovered" tube? A) Enzymes for amino acid consumption require O2 B) Bacteria must perform cellular respiration to use O2 C) Bacteria must not also be performing fermentation while consuming amino acids
A
How is this metabolic test innoculated? A) spread plating B) stab with a needle C) loop to broth transfer D) pasteur pipette
B
What are the results of the organism below on the OF test? A) Oxidation negative, fermentation positive, amino acid consumption unknown B) Oxidation negative, fermentation negative, amino acid consumption positive C) Oxidation negative, fermentation positive, amino acid consumption positive D) Oxidation positive, fermentation positive, amino acid consumption positive E) Oxidation positive, fermentation negative, amino acid consumption positive
B
What are the results of the organism below on the OF test? A) Oxidation +, Fermentation -, Amino Acid ? B) Oxidation +, Fermentation +, Amino Acid - C) Oxidation +, Fermentation +, Amino Acid ? D) Oxidation +, Fermentation+, Amino Acid +
C
What would a bacteria that could perform fermentation but not cellular respiration result in on this test? A) Yellow oxidation tube, yellow fermentation tube B) Yellow oxidation tube, green fermentation tube C) Green oxidation tube, yellow fermentation tube D) Green oxidation and fermentation tube
C
What are the results of the organism below on the OF test? A) Oxidation +, Fermentation +, Amino acid + B) Oxidation +, Fermentation -, Amino acid - C) Oxidation +, Fermentation, Amino acid + D) Oxidation +, Fermentation -, Amino Acid ?
D
What is the indicator used in this metabolic test? A) phenol red B) neutral red C) bromcresol purple D) bromthymol blue
D
Match the following outcome of the test to the final color of the media.
Yellow "uncovered" tube: performs cellular respiration using the sugar provided Yellow mineral oil overlay tube: performs fermentation using the sugar provided Green "uncovered" tube: does not perform cellular respiration using the sugar provided Green mineral oil overlay tube: does not perform fermentation using the sugar provided Blue top/green bottom "uncovered" tube: consumes amino acids, but does not perform cellular respiration on the sugar provided
Match the indicator's color change to the environmental condition in this test.
Yellow: Acidic Green: Neutral Blue: Basic
