Lab Four: Effect of pH and Temperature on Reaction Rate

In general, reaction rates increase every ___ to ___ times for every _______ degrees celsius rise in temperature.

2, 3 10

What is the optimal temperature for amylase?

37 degrees celsius

What is the optimal pH of amylase?

Around 6.8

How does the effect of temperature on Brownian movement influence enzyme activity?

Brownian movement increases as temperature increases. With an increase in the movement, there is an increase in molecular interactions. Thus, increasing the likelihood of an enzyme to come into contact with its substrate.

After a certain temperature is reached, what happens to the enzyme?

It is denatured. The active site is permanently altered

Denaturation of an enzyme caused by pH is ______________ whereas denaturation caused by temperature is ____________.

Reversible Irreversible

What is an optimum pH?

The pH at which reaction rate is fastest and takes the least amount of time

What determines an enzymes optimum pH?

The pH of fluids where the enzyme is found

What happens when an enzyme is placed in a solution with a different pH than its optimal pH?

The shape of the active site may be altered so significantly that the enzyme is denatured and cannot catalyze the reaction

What happens when you place a previously pH denatured enzyme into a solution with its optimal pH?

The shape of the active site will be restored