Lab Practicum #2 Question Set - 7. Oxidase Test

Cytochrome C Oxidase enzyme

What enzyme is being tested for in the Oxidase Test?

positive negative

71. Bacteria that belong to the family Pseudomonadaceae are oxidase __________________; bacteria that belong to the family Enterobacteriaceae are oxidase ________________________.

Right

Is the bacteria on the left or right positive for the Oxidase enzyme?

Purple within 20 seconds

72. What does a positive oxidase test look like? Include the time frame.

No color change within 20 seconds

73. What does a negative oxidase test look like? (2 answers)*

Neisseria N. meningitidis causes meningitis N. gonorrhoeae causes gonorrhea

70. The oxidase test that is useful in the presumptive identification of the gram negative coccus ________________________. Name 2 diseases caused by species of this genus.

Reagents will begin to oxidize independently given enough time, causing the color change

74. If your bacteria turn pink after more than one minute has elapsed since the oxidase reagent was added, why is this happening?