LS7A LaunchPad Week 9
Which primers would you use to amplify the light blue region of the circle?
1 and 3
The estimated number of genes in the human genome is:
25,000.
PCR Review Questions
3/3
VNTR Review Questions
4/4
The oligonucleotide primers used in the polymerase chain reaction are typically 20-30 nucleotides in length or longer; however, for purposes of this problem let's assume that six nucleotides is long enough. You wish to amplify the fragment shown below (the raised dots indicate several kilobases of DNA sequence not shown) and decide to design primers corresponding to the regions that are shown in bold. What primer sequences would you use?
5'-ACTTGC-3' and 5'-TGCCAC-3'
Gel Shift & Gene Regulation Practice Exam Questions
5/5
PCR Practice Exam Questions
5/5
Approximately what percentage of the human genome consists of repetitive DNA or transposable elements?
50%
Genome Variation & VNTR Practice Exam Questions
6/6
13.3 Review Questions
8/8
Only 2.5% of the human genome actually codes for proteins. The other 97.5% includes:
All of these choices are correct.
The accompanying gel diagram shows the bands obtained for a single variable number tandem repeat (VNTR) in evidence obtained at a crime scene (W) and genomic DNA from three suspects A-C. Which suspect CANNOT be ruled out as the source of the DNA in the sample?
All suspects can be ruled out.
What is the benefit of using Taq polymerase in PCR?
Because it is taken from bacteria that live in high temperatures, it stays active during denaturation steps of the reaction
One of the primers failed to bind the cDNA template:
False
The correct size target sequence was NOT amplified in this reaction:
False
Complex organisms can be characterized as having a:
None of the other answer options is correct.
Hypothesis: Protein A is a histone acetyltransferase (HAT).
The results do not provide enough information to support or reject this hypothesis
Hypothesis: Protein B represses (decreases) expression of Gene Y.
The results do not provide enough information to support or reject this hypothesis
Hypothesis: Protein A and Protein B can bind the same piece of DNA at the same time.
This hypothesis is rejected by the results
Hypothesis: Protein A can bind DNA.
This hypothesis is supported by the results
Hypothesis: Protein B can bind DNA.
This hypothesis is supported by the results
One of the primers was able to bind at two locations in the cDNA template:
True
_____ are short, repeated sequences of DNA that vary in number from one chromosome to the next.
Variable number tandem repeats
Given the actions listed below, which are all involved in PCR, select the answer that places them in the CORRECT order. extension initiation denaturation termination annealing
denaturation, annealing, extension
The C-value paradox applies to:
eukaryotes
The complexity of an organism is proportional to the number of genes in its genome.
false
The C-value paradox states that genome size:
is uncorrelated with complexity.
The polymerase chain reaction (PCR) is used to generate:
multiple copies of a targeted region of DNA.
Which suspect could be convicted based on these data alone?
neither
Can it be determined from the data in Table 13.1 which eukaryotic species has the largest genome? Can it be determined which species has the greatest number of chromosomes?
no, no
Having more than two sets of chromosomes in the genome is called:
polyploidy.
When DNA with variable number tandem repeats (VNTRs) is visualized on a gel, the resulting fragments separate according to their:
size.
A weapon (W) left behind at a crime scene is typed for a variable number tandem repeat (VNTR). The DNA from the weapon includes bands from the victim (V) as well as those from the perpetrator. Which of the suspects A-E has VNTR bands that are consistent with those of the perpetrator?
suspect B
The gel diagram below shows the bands obtained for a single variable number tandem repeat (VNTR) in evidence obtained at a crime scene (W) and genomic DNA from four suspects (A-E). Which suspect CANNOT be ruled out as the source of the DNA in the sample?
suspect D
Although a match between the DNA in a sample and the genomic DNA of a particular individual for a single variable number tandem repeat (VNTR) is not sufficient to establish identity, a mismatch is definitive. The primary reason is that:
the DNA in the sample must match its origin.
Imagine a region of DNA containing a variable number tandem repeat (VNTR) that is found in the genome of koalas. The possible number of VNTR alleles in the population equals the number of:
times a short noncoding sequence is repeated in tandem along the DNA.
