MICROSCOPY •

What are advantages to using a wet mount slide preparation?

A.The specimen could be viewed moving. B.The specimen behavior can be viewed D.The specimen may be alive.

There are three factors that determine the quality of a microscopic image:

magnification, resolution, contrast

Resolution

the ability to distinguish fine detail.

Contrast

the ability to distinguish objects, such as cells, from the background.

Magnification

the apparent increase in image size.

Cytology

the study of cells

What is the total magnification of a specimen using the 40X objective?

total magnification is 400X.

Which of the three factors affecting image quality is altered by the light source?

contrast

Fields of study where a microscope is a necessary tool include:

cytology histology micribiology pathology

Additional adjustments to the microscope can be made regarding lighting. These fine adjustments will not be addressed in this simulation.

-As magnification of the image increases, the light intensity decreases and will have to be adjusted. -The condenser lens focuses the light on the slide. -The sliding bar on the condenser lens is the aperture. As you move the aperture to decrease the light to the slide, the contrast increases. As you move the aperture to increase the light to the slide, the contrast decreases.

Microbiology

A field of biology that studies single and multicellular microscopic organisms.

The advantages of a wet mount may include:

Fast preparation. Requires no special equipment other than a transfer pipette, slide, and cover slip. Organisms may be viewed in their living state. Movement of motile microorganisms can be observed.

What is the advantage of starting with the 4X objective before moving to the 10X and then the 40X objective?

the field of view is larger, and it is easier to focus expmination:. The lower power objective has a larger field of view and is easier to focus.

How do the coarse and fine focus knobs work on a brightfield microscope?

the focus knob move the stage up and down

Total magnification

the overall enlargement of the image of a specimen. To calculate total magnification, multiply the magnification of the ocular lens (10X) with the magnification of the objective lens.

Why should the fine focus knob typically be used with the 10X and 40X objective lenses?

the slide should be close to in focus after focusing at the 4x objective. focusing with the coarse focus knob would result in too large a change in focus. explination:Using the coarse focus knob would result in a large change in focus, when only a small change is needed.

Pathology

the study of disease.

Histology

the study of tissues.