Microscopy Lab Quiz

Match the names of the microscope parts in column A with the descriptions in column B. Place the letter of your choice in the space provided. 1. Iris diaphram 2. Objective lens system 3. Stage 4. Adjustment knob 5. Condenser

1. Increases or decreases the light intensity 2. After light passes through the specimen, it next enters this lens system 3. Platform that supports a microscope slide 4. Causes stage (or objective lens) to move upward or downward 5. Concentrates light onto the specimen

What instrument did you use to weigh objects in the metric system lab? A. Scale B. Compass C. None of the answer choices are correct. D. Thermometer E. Ruler

A. Scale

If leaving an objective lens over the stage when storing the microscope, which objective lens should be placed over the stage? A. High power 40X B. Scanning 4X C. Oil immersion 100X D. Low power 10X

B. Scanning 4X

A change in what component of microscopy influences the apparent size of the objects being viewed? A. Contrast B. Magnification C. Light intensity

B. Magnification

The mechanical stage upper knob causes the stage to move A. Right and left. B. All of the answer choices are correct. C. Up and down. D. Back and forth.

D. Back and forth.

The fine adjustment knob on the microscope A. Locates the specimen. B. All of the answer choices are correct. C. Locates the focus plane. D. Fine-tunes the focus.

D. Fine-tunes the focus.

Which objective lens provides the highest total magnification? A. Low power B. High power C. Scan D. Oil immersion

D. Oil immersion

The mechanical stage lower knob causes the stage to move A. All of the answer choices are correct. B. Back and forth. C. Up and down. D. Right and left.

D. Right and left.

Which part of the microscope will be used first to adjust the focus when starting with the lowest power lens? A. Coarse focus B. Condenser C. Fine focus D. Ocular focus

A. Coarse focus

Which of the following is defined as the ability to distinguish an object from the background? A. Contrast B. Magnification C. Resolution D. Media type

A. Contrast

After focusing on an object with the low power objective lens, you are trying to bring it into focus under the 40x objective lens, but the field of view is very dark. Which of the following may remedy this problem? A. Increase the light intensity. B. Only use lower power magnification. C. Add more immersion oil. D. Adjust the fine adjustment knob.

A. Increase the light intensity.

When preparing a wet mount specimen for viewing, the specimen should be covered with A. A coverslip. B. Another glass slide. C. Clear paper. D. Transparent tape.

A. A coverslip.

The magnification of the ocular lens is A. 100x. B. 10x. C. 40x. D. 1x.

B. 10x.

If a specimen was being viewed using a 20x objective lens and 10x ocular lens, what would be the total magnification? A. 2000x B. 200x C. 30x D. 210x

B. 200x

The __________ causes the stage to move upward or downward. A. Mechanical stage B. Coarse adjustment knob C. Objective lens D. Iris diaphragm

B. Coarse adjustment knob

A wet mount requires the addition of certain dyes and cell fixatives approximately 12 hours before viewing the specimen. A. True B. False

B. False

The coarse adjustment knob on the microscope A. Locates the specimen. B. Locates the focus plane. C. All of the answer choices are correct. D. Fine-tunes the focus.

B. Locates the focus plane.

The mechanical stage upper knob causes the stage to move A. Up and down. B. Right and left. C. Back and forth. D. All of the answer choices are correct.

C. Back and forth.

Staining cells such that their color is different from the background enhances their A. Resolution. B. Media type. C. Contrast. D. Magnification.

C. Contrast.

Increasing the _____ would allow you to tell if what appears as one object is really two objects very near each other. A. Contrast B. Media type C. Resolution D. Magnification

C. Resolution

The objective lenses of the compound light microscope are attached to the A. Base. B. Body tube. C. Rotating nosepiece. D. Stage.

C. Rotating nosepiece.

Which objective lens provides the least total magnification? A. Oil immersion B. Low power C. Scan D. High power

C. Scan