Mouse Module

Which statement is TRUE regarding blood collection procedures in mice? Tail vein venipuncture requires anesthesia. Orbital sinus is a preferred route for blood collection because of animal welfare considerations. The lateral saphenous vein is not accessible in the mouse. Cardiac puncture is generally permitted only as a terminal procedure.

Cardiac puncture is generally permitted only as a terminal procedure.

Mice belonging to the genus Mus bred for use in research are regulated through PHS policy but are NOT covered by the Animal Welfare Act. True False

True

All of the following are appropriate components of an aseptic survival surgery EXCEPT: Beginning the surgery with instruments that have been cleaned with soap and water Scrubbing the surgical site with aseptic solution Surgeon wearing a gown and sterile gloves Clipping the surgical site on the animal

Beginning the surgery with instruments that have been cleaned with soap and water

What is the difference between CFA and IFA? CFA contains live Mycobacterium tuberculosis. CFA contains killed Mycobacterium tuberculosis. CFA contains live Mycobacterium pulmonis. CFA contains killed Mycobacterium pulmonis.

CFA contains killed Mycobacterium tuberculosis.

According to the AVMA Guidelines, which statement is true for the use of carbon dioxide as a euthanasia agent of mice? High concentrations of carbon dioxide are never distressful to the animals. Dry ice is an acceptable source of carbon dioxide for euthanasia. Death from carbon dioxide can be verified by a lack of respiration (apnea). Carbon dioxide poses minimal hazard to personnel when used with properly designed equipment.

Carbon dioxide poses minimal hazard to personnel when used with properly designed equipment.

Which statement is TRUE regarding care procedures for mice undergoing surgery? Antibiotics should be routinely administered to avoid wound infection. Supportive care and monitoring can be discontinued when the mice have recovered from anesthesia. Assessment of wound repair is the only objective of post-operative monitoring. During the procedure, mice should be monitored mainly for depth of anesthesia, cardiovascular and respiratory function.

During the procedure, mice should be monitored mainly for depth of anesthesia, cardiovascular and respiratory function.

Which statement is TRUE regarding anesthesia in mice? Long-term anesthesia is best achieved by repeat dosages of a short-acting injectable anesthetic cocktail. Ether is a preferred agent for gaseous anesthesia. Gas anesthesia (using a vaporizer) is generally the best option for providing extended anesthesia. Dose rates (mL/kg) of injectable agents are the same for the common laboratory animal species.

Gas anesthesia (using a vaporizer) is generally the best option for providing extended anesthesia.

Where is an IDEAL location for survival surgery on mice to take place? In a fume hood in a laboratory In an animal procedure room within the animal facility In the cage-changing hood in the housing room On a bench top in the laboratory

In an animal procedure room within the animal facility

If the ascites method of obtaining monoclonal antibody must be used, all of the following would likely decrease animal pain/distress EXCEPT: Limiting the amount of pristane used to prime the abdomen. Daily monitoring the degree of abdominal distension and respiratory distress caused by the ascites. Increasing the number of abdominal taps performed to prolong animal use. Using endpoint criteria tailored to mice with ascites.

Increasing the number of abdominal taps performed to prolong animal use.

Which of the following statements regarding quarantine/acclimation is FALSE? Quarantine provides the opportunity to detect infections that might not be apparent on delivery. The cost of delaying research protocols makes it important to move animals into the facility immediately after shipment. Investigator time, priceless research data, and animal lives can be lost due to infections. There are huge costs involved in controlling and eliminating infections once they escape into the animal facility.

The cost of delaying research protocols makes it important to move animals into the facility immediately after shipment.

Which of the following statements about adjuvants is FALSE? CFA must be used once only. CFA injections might put an animal into USDA pain category D requiring the use of post-injection analgesics or sedatives. The quantity of CFA adjuvant injected should be limited because of its toxic nature; however, IFA can be used in any quantity desired. Intramuscular injections of adjuvant in mice are discouraged.

The quantity of CFA adjuvant injected should be limited because of its toxic nature; however, IFA can be used in any quantity desired.

What precautions need to be taken in order to euthanize mice by carbon dioxide inhalation while ensuring the least amount of stress to the animals? Use a source of freshly generated carbon dioxide, such as dry ice. Prefill the chamber with carbon dioxide before placing the animals inside. Use a flow rate that displaces 30% to 70% of the chamber or cage volume/minute. Remove the animals from the chamber as soon as they stop moving.

Use a flow rate that displaces 30% to 70% of the chamber or cage volume/minute.

Fasting of mice before surgery is not necessary because: mice have small fat stores as energy reserve. mice are coprophagic. mice do not vomit.

mice do not vomit.

Mice require thermal support during anesthesia because: this is required by USDA regulations. mice lose body heat rapidly under anesthesia. mice have a low metabolic rate. None of the Above

mice lose body heat rapidly under anesthesia.

To monitor anesthetic depth, you should periodically: squeeze the animal's tail gently with your fingers. turn the anesthesia off and monitor the time it takes for the animal to wake up. pinch the animal's ear. pinch the animal's toe or footpad.

pinch the animal's toe or footpad.