Test 4 Chapter 18

Cells in the G0 state ________________. (a) do not divide. (b) cannot re-enter the cell cycle. (c) have entered this arrest state from either G1 or G2. (d) have duplicated their DNA.

(a)

Mitogens are _____. (a) extracellular signals that stimulate cell division. (b) transcription factors important for cyclin production. (c) kinases that cause cells to grow in size. (d) produced by mitotic cells to keep nearby neighboring cells from dividing

(a)

The principal microtubule-organizing center in animal cells is the ____________. (a) centrosome. (b) centromere. (c) kinetochore. (d) cell cortex.

(a)

Which of the following statements about kinetochores is true? (a) Kinetochores assemble onto chromosomes during late prophase. (b) Kinetochores contain DNA-binding proteins that recognize sequences at the telomere of the chromosome. (c) Kinetochore proteins bind to the tubulin molecules at the minus end of microtubules. (d) Kinetochores assemble on chromosomes that lack centromeres.

(a)

Which of the following statements about the cell cycle is false? (a) Once a cell decides to enter the cell cycle, the time from start to finish is the same in all eukaryotic cells. (b) An unfavorable environment can cause cells to arrest in G1. (c) A cell has more DNA during G2 than it did in G1. (d) The cleavage divisions that occur in an early embryo have short G1 and G2 phases.

(a)

Condensins ________________. (a) are degraded when cells enter M phase. (b) assemble into complexes on the DNA when phosphorylated by M-Cdk. (c) are involved in holding sister chromatids together. (d) bind to DNA before DNA replication begins.

(b)

Which of the following events does not usually occur during interphase? (a) Cells grow in size. (b) The nuclear envelope breaks down. (c) DNA is replicated. (d) The centrosomes are duplicated.

(b)

Which of the following statements is false? (a) DNA synthesis begins at origins of replication. (b) The loading of the origin recognition complexes (ORCs) is triggered by SCdk. (c) The phosphorylation and degradation of Cdc6 help to ensure that DNA is replicated only once in each cell cycle. (d) DNA synthesis can only begin after prereplicative complexes assemble on the ORCs.

(b)

Which organelle fragments during mitosis? (a) endoplasmic reticulum (b) Golgi apparatus (c) mitochondrion (d) chloroplast

(b)

You create cells with a version of Cdc6 that cannot be phosphorylated and thus cannot be degraded. Which of the following statements describes the likely consequence of this change in Cdc6? (a) Cells will enter S phase prematurely. (b) Cells will be unable to complete DNA synthesis. (c) The origin recognition complex (ORC) will be unable to bind to DNA. (d) Cdc6 will be produced inappropriately during M phase.

(b)

You have isolated a strain of mutant yeast cells that divides normally at 30°C but cannot enter M phase at 37°C. You have isolated its mitotic cyclin and mitotic Cdk and find that both proteins are produced and can form a normal M-Cdk complex at both temperatures. Which of the following temperature-sensitive mutations could not be responsible for the behavior of this strain of yeast? (a) inactivation of a protein kinase that acts on the mitotic Cdk kinase (b) inactivation of an enzyme that ubiquitylates M cyclin (c) inactivation of a phosphatase that acts on the mitotic Cdk kinase (d) a decrease in the levels of a transcriptional regulator required for producing sufficient amounts of M cyclin

(b) A cell with a mutation that prevents ubiquitylation of M cyclin could enter mitosis but could not exit mitosis properly.

A mutant yeast strain stops proliferating when shifted from 25°C to 37°C. When these cells are analyzed at the two different temperatures, using a machine that sorts cells according to the amount of DNA they contain, the graphs in Figure Q18-3 are obtained. Figure 18-3 Which of the following would not explain the results with the mutant? (a) inability to initiate DNA replication (b) inability to begin M phase (c) inability to activate proteins needed to enter S phase (d) inappropriate production of a signal that causes the cells to remain in G1

(b) At 37°C, the cells all have one genome-worth of DNA, meaning that they have not replicated their DNA and therefore have not entered S phase. Cells that are unable to begin M phase should have two genomes-worth of DNA, as they would have completed DNA replication and arrested in G2.

A cell with nuclear lamins that cannot be phosphorylated in M phase will be unable to ________________. (a) reassemble its nuclear envelope at telophase. (b) disassemble its nuclear lamina at prometaphase. (c) begin to assemble a mitotic spindle. (d) condense its chromosomes at prophase

(b) If the lamins cannot be phosphorylated during mitosis, the cells will be unable to disassemble their nuclear lamina, preventing the breakdown of the nuclear envelope at prometaphase. The mitotic spindle begins to form before the nuclear envelope breaks down, forming a sort of cage around the nucleus [choice (c)]. Lamins are not involved in chromosome condensation [choice (d)].

Apoptosis differs from necrosis in that necrosis ________________. (a) requires the reception of an extracellular signal. (b) causes DNA to fragment. (c) causes cells to swell and burst, whereas apoptotic cells shrink and condense. (d) involves a caspase cascade.

(c)

At the end of DNA replication, the sister chromatids are held together by the ___________. (a) kinetochores. (b) securins. (c) cohesins. (d) histones.

(c)

The Retinoblastoma (Rb) protein blocks cells from entering the cell cycle by ______. (a) phosphorylating Cdk. (b) marking cyclins for destruction by proteolysis. (c) inhibiting cyclin transcription. (d) activating apoptosis.

(c)

Which of the following descriptions is consistent with the behavior of a cell that lacks a protein required for a checkpoint mechanism that operates in G2? (a) The cell would be unable to enter M phase. (b) The cell would be unable to enter G2. (c) The cell would enter M phase under conditions when normal cells would not. (d) The cell would pass through M phase more slowly than normal cells

(c) Normal cells arrest at the G2 checkpoint if DNA replication is incomplete or DNA is damaged. Cells without this mechanism may enter M phase with unreplicated or damaged DNA, whereas normal cells would not.

Which of the following statements about the anaphase-promoting complex (APC) is false? (a) It promotes the degradation of proteins that regulate M phase. (b) It inhibits M-Cdk activity. (c) It is continuously active throughout the cell cycle. (d) M-Cdk stimulates its activity.

(c) The APC becomes activated in mid to late M phase.

How does S-Cdk help guarantee that replication occurs only once during each cell cycle? (a) It blocks the rise of Cdc6 concentrations early in G1. (b) It phosphorylates and inactivates DNA helicase. (c) It phosphorylates the Cdc6 protein, marking it for destruction. (d) It promotes the assembly of a prereplicative complex.

(c) The concentration of Cdc6 rises early in G1, independent of S-Cdk [choice (a)]. Cdc6 guides the assembly of the prereplicative complex at an origin [choice (d)]. By phosphorylating Cdc6, S-Cdk targets the protein for destruction, so that once an origin fires and replicates, Cdc6 will not be around to re-initiate replication. S-Cdk phosphorylates and activates DNA helicases [choice (b)].

The concentration of mitotic cyclin (M cyclin) ________________. (a) rises markedly during M phase. (b) is activated by phosphorylation. (c) falls toward the end of M phase as a result of ubiquitylation and degradation. (d) is highest in G1 phase.

(c) The concentration of mitotic cyclin rises gradually during G2 and it is ubiquitylated and degraded during late M phase.

You engineer yeast cells that express the M cyclin during S phase by replacing the promoter sequence of the M cyclin gene with that of S cyclin. Keeping in mind that yeast cells have one common Cdk that binds to all cyclins, which of the following outcomes is least likely during this experiment? (a) There will be both M cyclin-Cdk and S cyclin-Cdk complexes in the cell during S phase. (b) Some substrates that are normally phosphorylated in M phase will now be phosphorylated in S phase. (c) G1 cyclins will be expressed during S phase. (d) S-Cdk targets will be phosphorylated during S phase.

(c) The transcription of the G1 cyclins should not be affected by S-Cdk (which normally occurs in S phase) or M-Cdk (which likely now exists within the cell due to the inappropriate expression of M cyclin from the S cyclin promoter).

The G1 DNA damage checkpoint ________________. (a) causes cells to proceed through S phase more quickly. (b) involves the degradation of p53. (c) is activated by errors caused during DNA replication. (d) involves the inhibition of cyclin-Cdk complexes by p21.

(d)

Which of the following statements is false? (a) Mitotic Cdk must be phosphorylated by an activating kinase (Cak) before it is active. (b) Phosphorylation of mitotic Cdk by the inhibitory kinase (Wee1) makes the Cdk inactive, even if it is phosphorylated by the activating kinase. (c) Active M-Cdk phosphorylates the activating phosphatase (Cdc25) in a positive feedback loop. (d) The activating phosphatase (Cdc25) removes all phosphates from mitotic Cdk so that M-Cdk will be active.

(d)

Which of the following statements is true? (a) The mitotic spindle is largely made of intermediate filaments. (b) The contractile ring is made largely of microtubules and actin filaments. (c) The contractile ring divides the nucleus in two. (d) The mitotic spindle helps segregate the chromosomes to the two daughter cells.

(d)

Which word or phrase below best describes the phase in mitosis depicted in Figure Q18-41? (a) anaphase (b) prometaphase (c) S-phase checkpoint (d) metaphase

(d)

Which of the following is not good direct evidence that the cell-cycle control system is conserved through billions of years of divergent evolution? (a) A yeast cell lacking a Cdk function can use the human Cdk to substitute for its missing Cdk during the cell cycle. (b) The amino acid sequences of cyclins in plants are similar to the amino acid sequences of cyclins in humans. (c) The Cdk proteins in humans share conserved phosphorylation sites with the Cdk proteins in yeast. (d) Yeast cells have only one Cdk, whereas humans have many Cdks.

(d) Although it is true that yeast cells have one Cdk and human cells have many, this statement does not provide any evidence for the conservation of function across evolutionary time. All the other statements do provide such evidence.

Which letter is associated with the line that is pointing to the interpolar microtubules in Figure Q18-41? (a) A (b) B (c) C (d) D (e) E

(e)

Consider an animal cell that has eight chromosomes (four pairs of homologous chromosomes) in G1 phase. How many of each of the following structures will the cell have at mitotic prophase? A. sister chromatids B. centromeres C. kinetochores D. centrosomes E. centrioles

A—16; B—16; C—16; D—2; E—4

Name the stage of M phase in which the following events occur. Place the numbers 1-8 next to the letter headings to indicate the normal order of events. A. alignment of the chromosomes at the spindle equator B. attachment of spindle microtubules to chromosomes C. breakdown of nuclear envelope D. pinching of cell in two E. separation of two centrosomes and initiation of mitotic spindle assembly F. re-formation of the nuclear envelope G. condensation of the chromosomes H. separation of sister chromatids

A. 5, metaphase B. 4, prometaphase C. 3, prometaphase D. 8, cytokinesis E. 2, prophase F. 7, telophase G. 1, prophase H. 6, anaphase

Which of the following statements is false? (a) The cleavage furrow is a puckering of the plasma membrane caused by the constriction of a ring of filaments attached to the plasma membrane. (b) The cleavage furrow will not begin to form in the absence of a mitotic spindle. (c) The cleavage furrow always forms perpendicular to the interpolar microtubules. (d) The cleavage furrow always forms in the middle of the cell.

Choice (d) is false. Although the furrow always forms perpendicular to the interpolar microtubules about midway between the spindle poles [choice (c)], if the spindle were in an asymmetrical position (which can occur normally during development), cell division would not always occur in the middle of the cell.

Is the following statement true or false? After the nuclear envelope breaks down, microtubules gain access to the chromosomes and, every so often, a randomly probing microtubule captures a chromosome and ultimately connects to the kinetochore to become a kinetochore microtubule of the spindle.

the statement is true

Cytokinesis in animal cells ________________. (a) requires ATP. (b) leaves a small circular "scar" of actin filaments on the inner surface of the plasma membrane. (c) is often followed by phosphorylation of integrins in the plasma membrane. (d) is assisted by motor proteins that pull on microtubules attached to the cell cortex.

(a) All cell movement requires ATP, and in cytokinesis actin and myosin molecules are moving relative to one another to cause contraction of the contractile ring. The myosin is an ATPase that hydrolyzes ATP to power this movement. The assembly of the contractile ring requires a general rearrangement of the filaments in the cell cortex. The contractile ring in animal cells disassembles completely after mitosis, leaving no trace [choice (b)]. Phosphorylation of integrins (which weakens the hold of these transmembrane proteins on the extracellular matrix, thereby allowing cells to round up) generally precedes cytokinesis and is part of the general rearrangement of cell structure that accompanies cell division [choice (c)]. Microtubules do not have an important role in animal cytokinesis [choice (d)].

Which of the following does not occur during M phase in animal cells? (a) growth of the cell (b) condensation of chromosomes (c) breakdown of nuclear envelope (d) attachment of chromosomes to microtubules

(a) Cell size increases throughout interphase and not during M phase. All of the other phenomena are observed in M phase.

Programmed cell death occurs ________________. (a) by means of an intracellular suicide program. (b) rarely and selectively only during animal development. (c) only in unhealthy or abnormal cells. (d) only during embryonic development

(a) Programmed cell death results from an intracellular suicide program that eliminates unneeded, unwanted, or damaged cells. It occurs frequently and happens even in healthy cells throughout the lifetime of an individual.

Which of the following precede the re-formation of the nuclear envelope during M phase in animal cells? (a) assembly of the contractile ring (b) decondensation of chromosomes (c) reassembly of the nuclear lamina (d) transcription of nuclear genes

(a) The contractile ring in an animal cell begins to assemble in anaphase. The chromosomes do not decondense [choice (b)], the lamina does not re-form [choice (c)], and transcription does not begin [choice (d)] until the formation of the nuclear envelope is complete and nuclear proteins have been imported through the nuclear pores.

A friend declares that chromosomes are held at the metaphase plate by microtubules that push on each chromosome from opposite sides. Which of the following observations does not support your belief that the microtubules are pulling on the chromosomes? (a) the jiggling movement of chromosomes at the metaphase plate (b) the way in which chromosomes behave when the attachment between sister chromatids is severed (c) the way in which chromosomes behave when the attachment to one kinetochore is severed (d) the shape of chromosomes as they move toward the spindle poles at anaphase

(a) The jiggling movement [choice (a)] is simply a sign that the chromosomes are subject to forces from both sides, which could be the microtubules pulling, pushing, or both. All the other observations suggest pulling. When the attachment between sister chromatids is severed [choice (b)], both daughter chromosomes move toward their respective poles, which suggests that they are being pulled. When the attachment to one kinetochore is severed [choice (c)], the whole chromosome moves to the opposite pole, showing that the kinetochore microtubules are pulling on their attached chromatid, not pushing it. Similarly, the shape of the chromosomes as they move toward the pole [choice (d)] suggests that the chromosomes are being pulled.

Disassembly of the nuclear envelope ________________. (a) causes the inner nuclear membrane to separate from the outer nuclear membrane. (b) results in the conversion of the nuclear envelope into protein-free membrane vesicles. (c) is triggered by the phosphorylation of integrins. (d) must occur for kinetochore microtubules to form in animal cells.

(d) In animal cells, kinetochore microtubules cannot form if the chromosomes in the nucleus are separated from the microtubules in the cytoplasm because of the presence of the nuclear envelope. (But in some other cells, such as the yeast S. cerevisiae, the nuclear envelope never breaks down and yet chromosomes can attach to microtubules emanating from the spindle poles within the nucleus.) The nuclear envelope disassembles by breaking up into vesicles containing lipids from both the outer and inner envelopes [choice (a)]. Integral membrane proteins of the nuclear envelope and some of the nuclear lamins remain associated with the vesicles [choice (b)]. Phosphorylation of lamins (not integrins) triggers the breakdown of the nuclear lamina [choice (c)].

Which of the following statements is false? (a) Cytokinesis in plant cells is mediated by the microtubule cytoskeleton. (b) Small membrane vesicles derived from the Golgi apparatus deliver new cell-wall material for the new wall of the dividing cell. (c) The phragmoplast forms from the remains of interpolar microtubules of the mitotic spindle. (d) Motor proteins walking along the cytoskeleton are important for the contractile ring that guides formation of the new cell wall.

(d) No contractile ring is formed during plant cytokinesis.

What would be the most obvious outcome of repeated cell cycles consisting of S phase and M phase only? (a) Cells would not be able to replicate their DNA. (b) The mitotic spindle could not assemble. (c) Cells would get larger and larger. (d) The cells produced would get smaller and smaller.

(d) The cells produced would get smaller and smaller, as they would not have sufficient time to double their mass before dividing.

MPF activity was discovered when cytoplasm from a Xenopus M-phase cell was injected into Xenopus oocytes, inducing the oocytes to form a mitotic spindle. In a control experiment, Xenopus interphase cytoplasm was injected into oocytes and shown not to induce the formation of a mitotic spindle. Which of the following statements is not a legitimate conclusion from the control experiment? (a) The piercing of the oocyte membrane by a needle is insufficient to cause mitotic spindle formation. (b) An increased volume of cytoplasm is insufficient to cause mitotic spindle formation. (c) Injection of extra RNA molecules is insufficient to cause mitotic spindle formation. (d) Components of an interphase nucleus are insufficient to cause mitotic spindle formation.

(d) The interphase cytoplasmic extract used as the control would not have contained nuclear components (because the nuclear membrane was intact) and so one cannot conclude that components of an interphase nucleus are insufficient to cause mitotic spindle formation. The other statements are all true.

When introduced into mitotic cells, which of the following is expected to impair anaphase B but not anaphase A? (a) an antibody against myosin (b) ATPγS, a nonhydrolyzable ATP analog that binds to and inhibits ATPases (c) an antibody against the motor proteins that move from the plus end of microtubules to the minus end (d) an antibody against the motor proteins that move from the minus end of microtubules toward the plus end

(d) The motor proteins used in anaphase B to push the interpolar microtubules apart move toward the plus end of microtubules, whereas the motor proteins used in anaphase A move toward the minus end. Myosin [choice (a)] is not involved in either anaphase A or anaphase B. Motor proteins require ATP hydrolysis (they are ATPases) to function and are used in both anaphase A and anaphase B, so both types of anaphase will be affected by ATPγS [choice (b)]. The motor proteins used in anaphase A move toward the minus end of microtubules, as do the motor proteins used in anaphase B that are attached to the cell cortex [choice (c)].

Before chromosomes segregate in M phase, they and the segregation machinery must be appropriately prepared. Indicate whether the following statements are true or false. If false, change a single noun to make the statement true. A. Sister chromatids are held together by condensins from the time they arise by DNA replication until the time they separate at anaphase. B. Cohesins are required to make the chromosomes more compact and thus to prevent tangling between different chromosomes. C. The mitotic spindle is composed of actin filaments and myosin filaments. D. Microtubule-dependent motor proteins and microtubule polymerization and depolymerization are mainly responsible for the organized movements of chromosomes during mitosis. E. The centromere nucleates a radial array of microtubules called an aster, and its duplication is triggered by S-Cdk. F. Each centrosome contains a pair of centrioles and hundreds of γ-tubulin rings that nucleate the growth of microtubules.

A. False. Sister chromatids are held together by cohesins from the time they arise by DNA replication until the time they separate at anaphase. B. False. Condensins are required to make the chromosomes more compact and thus to prevent tangling between different chromosomes. C. False. The contractile ring is composed of actin filaments and myosin filaments. D. True. E. False. The centrosome nucleates a radial array of microtubules called an aster, and its duplication is triggered by S-Cdk. F. True.

Before a cell can enter M phase, two structures from Figure Q18-41 must be duplicated. Write the letters corresponding to the lines pointing to these structures, and write the names of the structures next to the letters.

A. centrosome; B. chromosome

Of the following mutations, which are likely to cause cell-cycle arrest? If you predict a cell-cycle arrest, indicate whether the cell will arrest in early G1, late G1, or G2. Explain your answers. A. a mutation in a gene encoding a cell-surface mitogen receptor that makes the receptor active even in the absence of the mitogen B. a mutation that destroyed the kinase activity of S-Cdk C. a mutation that allowed G1-Cdk to be active independently of its phosphorylation status D. a mutation that removed the phosphorylation sites on the Rb protein E. a mutation that inhibited the activity of Rb

B and D are likely to cause cell-cycle arrest. A. Because ligand-independent activation of a mitogen receptor will probably make the cell divide when it otherwise might not, this mutation is unlikely to cause a cell-cycle arrest. B. This mutation is likely to cause cell-cycle arrest in late G1. Without S-Cdk activity, the cells will probably be unable to enter S phase. C. Phosphorylation-independent activity of G1-Cdk is unlikely to lead to cell-cycle arrest; the cells should progress through the cycle, although the kinetics and fine regulation of the cycle may be altered. D. This mutation is likely to cause cell-cycle arrest in early G1. Unphosphorylated Rb will inhibit the transcription of genes required for progression through G1 and into S phase. This inhibition is normally released on phosphorylation of Rb. If Rb cannot be phosphorylated, it will always inhibit transcription of these genes, leading to arrest in early G1. E. If Rb is inactivated by a mutation, cells will be more likely to divide in the absence of extracellular mitogens, which is the opposite of a cell-cycle arrest

Irradiated mammalian cells usually stop dividing and arrest at a G1 checkpoint. Place the following events in the order in which they occur. A. production of p21 B. DNA damage C. inhibition of cyclin-Cdk complexes D. accumulation and activation of p53

B, D, A, C

The cytoskeleton of an animal cell changes markedly between G1 and early M phase (prophase) of the cell cycle. For each of the following sentences, choose one of the options enclosed in square brackets that best describes the changes to the cytoskeleton and its components. Before mitosis, the number of centrosomes must [increase/decrease]. At the beginning of [anaphase/prophase] in animal cells, the centrosomes separate in a process driven partly by interactions between the [plus/minus] ends of microtubules arising from the two centrosomes. Centrosome separation initiates the assembly of the bipolar mitotic spindle and is associated with a sudden [increase/decrease] in the dynamic instability of microtubules. In comparison with an interphase microtubule array, a mitotic aster contains a [smaller/larger] number of [longer/shorter] microtubules. Extracts from M-phase cells exhibit [increased/decreased/unchanged] rates of microtubule polymerization and increased frequencies of microtubule [shrinkage/growth]. The changes in microtubule dynamics are largely due to [enhanced/reduced] activity of microtubule-associated proteins and [increased/decreased] activity of catastrophins. The new balance between polymerization and depolymerization of microtubules is necessary for the mitotic spindle to move the [replicated chromosomes/daughter chromosomes] to the metaphase plate.

Before mitosis, the number of centrosomes must increase. At the beginning of prophase in animal cells, the centrosomes separate in a process driven partly by interactions between the plus ends of microtubules arising from the two centrosomes. Centrosome separation initiates the assembly of the bipolar mitotic spindle and is associated with a sudden increase in the dynamic instability of microtubules. In comparison with an interphase microtubule array, a mitotic aster contains a larger number of shorter microtubules. Extracts from M-phase cells exhibit unchanged rates of microtubule polymerization and increased frequencies of microtubule shrinkage. The changes in microtubule dynamics are largely due to reduced activity of microtubule-associated proteins and increased activity of catastrophins. The new balance between polymerization and depolymerization of microtubules is necessary for the mitotic spindle to move the replicated chromosomes to the metaphase plate.

Examine the schematic representation of centrosome duplication in Figure Q1844. By analogy with DNA replication, would you classify centrosome duplication as conservative or semiconservative? Explain your answer. Figure Q18-44

Centrosome duplication is semiconservative. The paired centrioles in the centrosome separate, and each serves to nucleate the assembly of a new centriole. As a consequence, each new centrosome consists of one old and one new centriole. Thus, centrosome duplication is analogous to DNA replication, in which the new double helix consists of one old DNA strand and one newly replicated DNA strand.

Progression through the cell cycle requires a cyclin to bind to a Cdk because _________. (a) the cyclins are the molecules with the enzymatic activity in the complex. (b) the binding of a cyclin to Cdk is required for Cdk enzymatic activity. (c) cyclin binding inhibits Cdk activity until the appropriate time in the cell cycle. (d) without cyclin binding, a cell-cycle checkpoint will be activated.

Choice (b) is correct. Cyclins have no enzymatic activities themselves [choice (a)], and cyclin binding to Cdk activates the Cdk [choice (c)]. As far as we know, cyclin-Cdk binding is not directly monitored by checkpoints [choice (d)].

Which of the following statements is true? (a) Anaphase A must be completed before anaphase B can take place. (b) In cells in which anaphase B predominates, the spindle will elongate much less than in cells in which anaphase A dominates. (c) In anaphase A, both kinetochore and interpolar microtubules shorten. (d) In anaphase B, microtubules associated with the cell cortex shorten.

Choice (d) is correct. Anaphase A and anaphase B generally occur at the same time [choice (a)]. In cells in which anaphase B predominates, the spindle will elongate more than in cells in which anaphase A predominates [choice (b)]. In anaphase A, only the kinetochore microtubules shorten [choice (c)].

Which of the following statements about apoptosis is true? (a) Cells that constitutively express Bcl2 will be more prone to undergo apoptosis. (b) The prodomain of procaspases contains the catalytic activity necessary for procaspase activation. (c) Bax and Bak promote apoptosis by binding to procaspases in the apoptosome. (d) Apoptosis is promoted by the release of cytochrome c into the cytosol from mitochondria.

Choice (d) is correct. Bcl2 tends to inhibit rather than promote apoptosis [choice (a)]. The prodomain of procaspases does not contain the catalytic activity needed for procaspase activation and is usually discarded from the active caspase [choice (b)]. When activated, Bax and Bak promote apoptosis by stimulating the release of cytochrome c from mitochondria into the cytosol, not by binding to procaspases in the apoptosome [choice (c)].

Levels of Cdk activity change during the cell cycle, in part because ________________. (a) the Cdks phosphorylate each other. (b) the Cdks activate the cyclins. (c) Cdk degradation precedes entry into the next phase of the cell cycle. (d) cyclin levels change during the cycle.

Choice (d) is correct. Cdks do not phosphorylate each other [choice (a)]. The Cdks do not activate the cyclins [choice (b)], and Cdks are not degraded during specific phases of the cycle [choice (c)]. The cyclins, however, are degraded in a cell-cycle-dependent fashion, and they are required for Cdk activity.

In which phase of the cell cycle do cells check to determine whether the DNA is fully and correctly replicated? (a) at the transition between G1 and S (b) when cells enter G0 (c) during M (d) at the end of G2

Choice (d) is correct. It does not make sense to monitor DNA replication before S phase because DNA replication has not yet occurred [choice (a)]. When cells enter G0, they do not replicate their DNA [choice (b)]. During M phase, chromosomes are condensed for chromosome segregation, so it would be difficult for the cell to examine the replicated DNA for errors at that point [choice (c)].

What is the main molecular difference between cells in a G0 state and cells that have simply paused in G1?

In G0, the cell-cycle control system is partly dismantled, so that some of the Cdks and cyclins are not present. Cells paused in G1, by contrast, still contain all the components of the cell-cycle control system. Whereas the latter cells can rapidly progress through the cycle when conditions are right, G0 cells need to synthesize the missing cell-cycle control proteins so as to re-enter the cycle, which usually takes 8 hours or more.

Many features of __________________ cells make them suitable for biochemical studies of the cell-cycle control system. For example, the cells are unusually large and are arrested in a __________________-like phase. When the cells are triggered to resume cycling, the cell divisions have especially __________________ G1 and G2 phases and occur __________________. Studies with Xenopus eggs identified a partly purified activity called __________________ that drives a resting Xenopus oocyte into M phase. MPF activity was found to __________________ during the cell cycle, although the amount of its kinase component, called __________________, remained constant. The regulatory component of MPF, called __________________, has a __________________ effect on MPF activity and plays a part in regulating interactions with its __________________s. The components of MPF are evolutionarily __________________ from yeast to humans, so that the corresponding human genes are __________________ to function in yeast. able hexokinase short asynchronously inhibitory sperm Cdk long steady conserved M stimulatory cyclin maturation promoting factor substrate divergent oscillate synchronously egg PI 3-kinase ubiquitin fibroblast regulin unable G1 S uniform G2

Many features of egg cells make them suitable for biochemical studies of the cell-cycle control system. For example, the cells are unusually large and are arrested in a G2-like phase. When the cells are triggered to resume cycling, the cell divisions have especially short G1 and G2 phases and occur synchronously. Studies with Xenopus eggs identified a partly purified activity called maturation promoting factor that drives a resting Xenopus oocyte into M phase. MPF activity was found to be oscillate during the cell cycle, although the amount of its kinase component, called Cdk, remained constant. The regulatory component of MPF, called cyclin, has a stimulatory effect on MPF activity and plays a part in regulating interactions with its substrates. The components of MPF are evolutionarily conserved from yeast to humans, so that the corresponding human genes are able to function in yeast.

For each of the following sentences, fill in the blanks with the best word or phrase selected from the list below. Not all words or phrases will be used; each word or phrase should be used only once. The cell cycle consists of an alternation between __________________, which appears as a period of dramatic activity under the microscope, and a preparative period called __________________, which consists of three phases called __________________, __________________, and __________________. During M phase, the nucleus divides in a process called __________________, and the cytoplasm splits in two in a process called __________________. The cell-cycle control system relies on sharp increases in the activities of regulatory proteins called __________________, or __________________, to trigger S phase and M phase. Inactivation of __________________ is required to exit from M phase after chromosome segregation. APC G2 phase metaphase Cdks interphase microtubules condensation intraphase mitosis cyclin-dependent kinases kinesins myosins cytokinesis M phase S phase G1 phase M-Cdk S-Cdk G1-Cdk meiosis

The cell cycle consists of an alternation between M phase, which appears as a period of dramatic activity under the microscope, and a preparative period called interphase, which consists of three phases called G1 phase, S phase, and G2 phase. During M phase, the nucleus divides in a process called mitosis, and the cytoplasm splits in two in a process called cytokinesis. The cell-cycle control system relies on sharp increases in the activities of regulatory proteins called cyclin-dependent kinases, or Cdks, to trigger S phase and M phase. Inactivation of M-Cdk is required to exit from M phase after chromosome segregation.

The four phases of the cell cycle, in order, are G1, __________________, __________________, and __________________. A cell contains the most DNA after __________________ phase of the cell cycle. A cell is smallest in size after __________________ phase of the cell cycle. Growth occurs in __________________, __________________, and __________________ phases of the cell cycle. A cell does not enter mitosis until it has completed __________________ synthesis. DNA M protein G1 nucleotide S G2 organelle

The four phases of the cell cycle, in order, are G1, S, G2, and M. A cell contains the most DNA after S phase of the cell cycle. A cell is smallest in size after M phase of the cell cycle. Growth occurs in G1, S, and G2 phases of the cell cycle. A cell does not enter mitosis until it has completed DNA synthesis

The survival, __________________, and size of each cell in an animal are controlled by extracellular signal molecules secreted by neighboring and distant cells. Many of these signal molecules bind to a cell-surface __________________ and trigger various intracellular signaling pathways. One class of signal molecules, called __________________, stimulates cell division by releasing the molecular brakes that keep cells in the __________________ or __________________ phase of the cell cycle. Members of a second class of signal molecules are called __________________, because they stimulate cell growth and an increase in cell mass. The third class of signal molecules, called __________________, inhibits __________________ by regulating members of the __________________ family of proteins. In addition to such stimulatory factors, some signal proteins such as __________________ act negatively on other cells, inhibiting their survival, growth, or proliferation. anaphase differentiation myostatin annihilation G0 nourishment apoptosis G1 nutrition arrestase G2 phosphatases Bcl2 growth factors proliferation biosynthetic interphase receptor cascades ligand S caspase M survival factors Cdk mitogens transcription cyclin

The survival, proliferation, and size of each cell in an animal are controlled by extracellular signal molecules secreted by neighboring and distant cells. Many of these signal molecules bind to a cell-surface receptor and trigger various intracellular signaling pathways. One class of signal molecules, called mitogens, stimulates cell division by releasing the molecular brakes that keep cells in the G0 or G1 phase of the cell cycle. Members of a second class of signal molecules are called growth factors, because they stimulate cell growth and an increase in cell mass. The third class of signal molecules, called survival factors, inhibits apoptosis by regulating members of the Bcl2 family of proteins. In addition to such stimulatory factors, some signal proteins such as myostatin act negatively on other cells, inhibiting their survival, growth, or proliferation.