Urea Broth

to see if the bacteria produces the enzyme urease, thus the major production of ammonia

What is the purpose of the urea broth test?

positive

Is this a negative or positive test for urea broth test? (the tube on the right)

negative

Is this a negative or positive test for urea broth test? (the tube on the left)

Urease

Urea Broth Test: what enzyme/metabolism is used?

Urease converts urea into ammonia which is alkaline The pH indicator phenol red changes to hot pink when it becomes basic over pH 8.0 yet stays peach color at pH 6.6 were it is acidic

For urea broth test what is the chemical process?

Positive: the media changes color to hot pink/cerise Negative: the media has no color change, stays at a peach color?

For urea broth test what states if it is a positive test or a negative test?

It is a pH indicator changes to hot pink when it becomes basic over pH 8.0 stays peach color at pH 6.6 were it is acidic

For urea broth test, what does the phenol red in the media do?

Urea Broth

Urea Broth Test: what media is used?

media: Urea Broth enzyme: Urease reagent: Phenol red in medium

Urea Broth Test: what media is used? what enzyme/metabolism is used? What is the reagent?

Phenol red in medium

Urea BrothTest: What is the reagent?