3.3
A prosthetic group is best described by which of the following statements? Question 5 options: A molecule necessary for a protein to perform its function A protein that must bind some other molecule in order to function A post-translational modification A non-protein molecule that is permanently incorporated into the protein's native structure
A molecule necessary for a protein to perform its function WRONG
Which of the following statements correctly defines a cofactor? Question 1 options: A molecule that assists in fixing misfolded proteins One of the chains of a protein that contains several peptide sequences A non-protein component incorporated into the protein structure A short peptide that helps fold a protein
A non-protein component incorporated into the protein structure
A protease is best described by which of the following statements? Question 2 options: A protein this is cut up by other proteins A protein involved in the synthesis of other proteins A protein that catalyzes hydrolysis of peptide bonds A protein that is not yet completely synthesized
A protein that catalyzes hydrolysis of peptide bonds
Which of the following is an example of a post-translational modification? Adding a phosphate group to a polysaccharide Processing of mRNA to remove portions of the mRNA sequence Removing the first 5 amino acid residues from a folded protein All of these
Adding a phosphate group to a polysaccharide (WRONG)
Which of the following molecules is an example of glycosylation? Question 5 options: N-linked oligosaccharide All of these Proteoglycan O-linked oligosaccharide
All
NMR spectroscopy is useful for obtaining which of the following information? Question 3 options: Motions in a protein Quaternary structure of large proteins Primary structure All of these
All (WRONG)
Which of the following reactions is an example of a post-translational modification? Question 1 options: Adding an additional amino acid to the C terminus of a growing protein Addition of an acetyl group to the NH3 group of a lysine not part of a protein Addition of a phosphate group to an OH side chain within a protein All of these
All (WRONG)
How are cofactors and prosthetic groups related? The two terms have nothing to do with each other All cofactors are prosthetic groups All prosthetic groups are cofactors The two terms refer to the same thing
All prosthetic groups are cofactors
The term molten globule refers to which of the following items? A misfolded protein The final, correctly folded protein structure An intermediate state during protein folding A protein missing a cofactor
An intermediate state during protein folding
An N-linked oligosaccharide is described which of the following statements? Question 3 options: None of these An oligosaccharide made up of monomers connected through nitrogen atoms An oligosaccharide that is bonded to another oligosaccharide via a nitrogen atom An oligosaccharide bonded to a nitrogen atom in a protein
An oligosaccharide bonded to a nitrogen atom in a protein
Which of the following amino acids can be N-linked to saccharides (select all correct answers)? Question 5 options: Lys Gln Arg Asn
Asn
Which of the following chemical entities is a type of cofactor? Question 5 options: Metal ion Neither of these Prosthetic group Both of these
Both
Which of the following terms is a type of saccharide attachment to proteins? N-linked O-linked Neither of these Both of these
Both of these
Which of the following terms is a non-protein component that contributes to protein structure? Molten globule Cofactor Molecular chaperone Apo
Cofactor
Post-translational modifications are described by which of the following processes? Question 5 options: Covalent modification to amino acid residues after protein synthesis The replacement of one complete amino acid by another in a protein mRNA splicing A change to the codons that code for a protein
Covalent modification to amino acid residues after protein synthesis
Which of the following techniques provides direct information about 3-D motions in proteins? NMR spectroscopy X-ray crystallography Cryo electron microscropy Mass spectrometry
Cryo electron microscropy( Wrong) Cryo electron microscropy determines 3D structures of complexes and individual proteins by freezing samples NMR determines 3d structure in soln w/ motion. Mass spectrometry is for the secondary structure Xray crystallography is the oldest of 3D viewing. Molecules in same conformation are crystallized and electrons scatter xrays to measure structure
A protein that is misfolded can also be described as which of the following terms? Question 3 options: Denatured Native Confused Degraded
Denatured
Which of the following terms describes a protein that is in a non-functional form? Question 3 options: Prosthetic Denatured Native Globular
Denatured
Which of the following terms describes a protein containing all necessary cofactors? Question 4 options: Denatured Factorless Apo Holo
Holo
Proteoglycan is a major component of which of the following structures? Question 1 options: Human extracellular matrix Wood Molecular chaperones Bacterial cell walls
Human Extracellular matrix
Which of the following techniques provides information about protein sequence (select all correct answers)? Question 4 options: NMR spectroscopy Cryo electron microscropy Mass spectrometry X-ray crystallography
Mass Spectrometry
Which of the following techniques is useful for determining primary structure? NMR spectroscopy Mass spectrometry Cryo electron microscropy X-ray crystallography
Mass spectrometry X-ray crystallography is the used to view 3D sructures NMR Spectroscopy is used to determine small protein structure in motion w/n a soln. Mass spectrometry shows the primary structure
Which of the following techniques is least useful for determining tertiary structure? Question 5 options: Cryo electron microscropy NMR spectroscopy Mass spectrometry X-ray crystallography
Mass spectroscopy
Which of the following terms describes the molecules and functional groups surrounding a protein residue? Question 1 options: Globular units Microenvironment Cofactors Domain
Microenvironment
Which of the following terms is an intermediate state during protein folding in which the protein has adopted the general shape of the final structure α-helix Random coil Native Molten globule
Molten Globule
Asn can be post-translationally modified by a saccharide through which of the following methods? None of these O-linked H-bonded N-linked All of these
N-linked
Which of the following methods is correctly matched to a type of structure that it can be used to determine? Mass spectrometry - secondary structure All of these Cryo electron microscopy - primary structure NMR spectroscopy - quaternary structure
NMR spectroscopy - quaternary structure
Which of the following terms describes a protein folded into a functional form? Random coil Denatured Native Globular
Native
The microenvironment of an amino acid residue includes which of the following elements? Question 1 options: pH All of these Distant amino acids Nearby amino acids
Nearby amino acids Microenvironment is the surrounding. So distant is not correct. pH is a property of the entire solution.
Ser can be post-translationally modified by a saccharide through which of the following methods? Question 2 options: N-linked All of these O-linked H-bonded
O-linked
Glu can be post-translationally modified by a saccharide through which of the following methods? All of these None of these O-linked N-linked H-bonded
O-linked (WRONG)
NMR spectroscopy can provide which of the following types of structural information for proteins (select all correct answers)? Question 3 options: Primary Tertiary Quaternary Secondary
PREV -2,4
An O-linked oligosaccharide is described which of the following statements? An oligosaccharide made up of monomers connected through oxygen atoms An oligosaccharide that is bonded to another oligosaccharide via an oxygen atom An oligosaccharide bonded to an oxygen atom in a protein None of these
PREV 1
For a particular molecule X, which of the following statements describes the microenvironment? Question 5 options: All of these The molecules and functional groups surrounding X The solvent that X is in The functional groups in X
PREV 1
The microenvironment of an amino acid residue includes which of the following elements? None of these Polarity of the amino acid residue Nearby water molecules Distant ions in solution
PREV 1
Which of the following types of monomers is incorporated into peptidoglycan (select all correct answers)? Question 3 options: Nucleotides Saccharides Lipids Amino acids
PREV 124
If a protein is described as being apo, which of the following statements is true? Question 4 options: The protein does not need cofactors The protein lacks one or more required cofactors The protein contains all required cofactors The protein is artificial
PREV 3
Which of the following terms describes the addition of a saccharide chain to an amino acid chain? Question 2 options: Glycosylation Polysaccharide bond Glycosidic addition Peptidoglycan
PREV 4
Cryo electron microscopy can provide which of the following types of structural information for proteins (select all correct answers)? Question 4 options: Primary Quaternary Tertiary Secondary
PREV-Tertiary,Secondary
Which of the following terms describes a protein lacking a necessary cofactor? Question 3 options: Holo Apo Factorless Denatured
PREV4
Which of the following terms describes a saccharide linked to an amino acid chain? Starch Peptidoglycan Glycan Glycogen
Peptidoglycan
Which of the following techniques measures the 3-D position of hydrogen atoms in solution (select all correct answers)? Question 1 options: Cryo electron microscropy Mass spectrometry NMR spectroscopy X-ray crystallography
Prev 4
Mass spectrometry typically provides which of the following types of structural information for proteins (select all correct answers)? Question 1 options: Primary Tertiary Quaternary Secondary
Primary
Which of the following molecules is a type of cofactor? Question 4 options: Subunit Prosthetic group None of these Water
Prosthetic group Cofactors include metal ion, small molecules, and prosthetic groups
Which of the following terms describes an enzyme that catalyzes hydrolysis of peptide bonds? Question 1 options: Peptidase Cutase Bondase Protease
Protease
Mass spectrometry typically provides which of the following types of structural information for proteins? Question 2 options: Side chain interactions Sequence Motifs Backbone rotations
Sequence
Which of the following amino acids can be O-linked to saccharides Asn Ser Tyr Thr
Ser Thr
Which type of structure describes interactions between side chains of amino acid residues and a cofactor? Question 2 options: Secondary Tertiary Quaternary Primary
Tertiary
X-ray crystallography can be used to learn about which type of structure (select all correct answers)? Question options: Primary Quaternary Secondary Tertiary
Tertiary (Wrong)
X-ray crystallography is useful for obtaining which of the following information? Primary structure Tertiary structure Motions in a protein All of these
Tertiary structure
If a protein is described as being holo, which of the following statements is true? The protein lacks one or more required cofactors The protein contains all required cofactors The protein is artificial The protein does not need cofactors
The protein contains all required cofactors
If a protein (or a portion of a protein) is a random coil, which of the following statements is true? Question 1 options: The protein ties itself into knots The protein can stretch like a spring The protein forms a coil, but only sometimes The protein has no defined 3-D structure
The protein has no defined 3-D structure
Which of the following features is essential in the native structure of a protein? Question 4 options: All of these The protein is able to perform its function The protein is rigid The protein has regular secondary structure
The protein is able to perform its function
N-linked saccharides are found bonded to which of the following locations (select all that apply)? Question 2 options: The backbone oxygen of serine The N-terminus nitrogen atom of a protein The side chain nitrogen of asparagine Any side chain nitrogen of arginine
The side chain nitrogen of asparagine
O-linked saccharides are found bonded to which of the following locations (select all that apply)? The C-terminus oxygen atoms of a protein Either side chain oxygen of aspartic acid The side chain oxygen of serine The backbone oxygen of glycine
The side chain oxygen of serine Olinked are Ser & Thr
A molecular chaperone has which of the following functions? To assist proteins in correctly folding To synthesize proteins To add post-translational modifications to proteins To break peptide bonds and degrade proteins
To assist proteins in correctly folding Chaperone proteins helps correct folding of a protein on its way to native structure
When is a protein in its native structure? Question 2 options: When tertiary structure is present When the protein is in the functionally active structure When it is randomly folded When it has a 3-D structure
When the protein is in the functionally active structure
Which of the following techniques measures the 3-D position of atoms in a static arrangement (select all correct answers)? Mass spectrometry X-ray crystallography NMR spectroscopy Cryo electron microscropy
X-ray crystallography Cryo electron microscropy