A&P I, Lab 5: Microscopes & slide prep
The human eye cannot perceive objects less than __________?
0.1 millimeter
What is the total magnification when the oil immersion (100X) objective is used? Assume magnification of eyepiece is 10x.
1000x
The total magnification achieved using a 10x objective lens with a 10x eyepiece lens is __________.
100x
What is the total magnification when the low power (10X) objective is used? Assume magnification of eyepiece is 10x.
100x
What is the magnification of the eyepiece (ocular lens)?
10x
Low-power objective
10x lens magnification, 100x total magnification. Used after scanning lens for bringing specimen into focus.
A micrometer equals 1/1,000 of a millimeter and is symbolized by µm. In micrometers, what is the diameter of the scanning power field of view?
4,500 micrometers
What is the total magnification when the high power (40X) objective is used? Assume magnification of eyepiece is 10x.
400x
What is the total magnification when the scanning (4X) objective is used? Assume magnification of eyepiece is 10x.
40x
High-power objective
40x lens magnification, 400x total magnification. Most detail is observed. Use ONLY fine adjustment knob to focus.
Scanning objective
4x lens magnification , 40x total magnification. Faces the microscope stage upon storage. Used for initially bringing specimen into focus.
When preparing a wet mount specimen for viewing, it should be covered with __________.
A coverslip
If a circular object or specimen extends halfway across the scanning field, what is its diameter in millimeters?
About 2.2 millimeters
In millimeters, what is the diameter of the scanning field of view?
About 4.5 mm using a 4x objective.
Adjustment knob
Causes the stage (or objective lens) to move upward or downward.
Condenser
Concentrates light onto the specimen.
Base
Flat surface that supports the microscope.
When creating a microscope slide to view a wet sample, from how far you should drop the coverslip, and why?
From a 45-degree angle, to prevent air bubbles.
Stage (slide) clip
Holds the microscope slide in position.
What does the lens system do to the image of a numeral or letter?
Image is reversed from right to left
Iris diaphragm
Increases or decreases the light intensity.
The __________ increases or decreases the light intensity of the compound light microscope.
Iris diaphragm
Substage illuminator
Lamp; directs light up thru the specimen.
Basic lens cleaning is accomplished using __________.
Lens paper
What change occurred in the intensity of the light in the field of view when you exchanged the low-power objective for the high-power objective?
Light intensity decreased
After light passes through the specimen, it next enters the __________.
Objective lens system
Nosepiece
Part to which the objective lenses are attached.
Stage
Platform that supports a microscope slide.
The objective lenses of the compound light microscope are attached to the __________?
Rotating nosepiece
Which objective lens provides the least total magnification?
Scanning
Which objectives are used to get a specimen in focus?
Scanning, followed by low-power
Arm
Serves as a handle for carrying the microscope.
Which microscope is a good option to use for viewing rather large, opaque specimens?
Stereo microscope
Field of view
The circular visible area of the specimen seen through the eyepiece.
T/F: When cleaning the lenses of a microscope, it is important to use lens paper or a high-quality cotton swab.
True
