all of the quizzes done in BIOL 3201 lab
microorganisms are everywhere
"Ubiquity of microorganisms" means...
pink
gram negative bacteria are what color
purple
gram positive bacteria are what color
red, indole
A ____ color on the addition of Kovac's reagent to a tryptophan tube or SIM tube indicates a positive ____ test.
true
A blue citrate tube results from your organism producing ammonia leading to an alkaline pH on the slant. (true or false)
blue
A positive oxidase reaction is evidenced by the development of a _____ color.
differential medium
A/an _______ medium contains substances that cause some bacteria to take on an appearance that distinguishes it from other bacteria.
no reagent is necessary
After incubation, ____ is added to the urea tube to test for urea hydrolysis.
grams iodine
After incubation, _____ is added to the starch plate to detect starch hydrolysis.
ferric chloride, green
After the addition of _______, a phenylalanine slant that has an organism that produces phenylalanine deaminase will turn _____.
true
UV light is routinely used to sterilize surfaces. (true)
260 nm
UV radiation at ____ is the most germicidal because it is the wavelength at which DNA maximally absorbs UV light.
strict anaerobes
Bacteria that can not tolerate oxygen and must be cultured under conditions in which oxygen is completely eliminated, otherwise they will be harmed or killed.
alkaliphiles
Bacteria that grow at pH > 7.
psychotrophs
Bacteria that grow at temperatures lower than their optimal temperature range.
bsl 4
Biosafety level designation used to describe microorganisms that are dangerous and exotic with high risk of aerosol transmitted infections. Rarely are there treatments or vaccines for these microorganisms, and the diseases they cause are frequently fatal.
oculars, objectives, condenser
Brightfield microscopes have three lens systems:....
strict anaerobes
Clostridium are an example of _______.
condenser
Collects and directs the light from the light source to the slide and is located under the stage of a microscope.
safranin
Counter stain for Gram stain.
ethanol
Decolorizer for Gram stain.
blue
Utilization of citrate by an organism will turn a citrate tube _____.
cytosine or thymine
When DNA absorbs UV light, pyrimidine dimers are formed. These form when a covalent bond forms between two adjacent ________ molecules in a DNA strand.
red to yellow
When carbohydrates are fermented in the presence of phenol red (for example in a Phenol red glucose tube) the liquid's color changes from ___ to ____.
40x and 100x
When focusing the ___ objective(s), you only adjust the fine focus adjustment/knob.
leave it on the counter
When inoculating a Petri plate, what do you do with the lid of the plate?
add zinc
You carryout a nitrate reduction test on a bacterium. After addition of reagent A and reagent B, the test appears negative (no color change). What should you do next?
lens wipes
You use ____ to clean the oculars, objectives, and stage of a microscope.
bsl 3
Lab coats, gloves, and respirators must be worn as personal protective equipment (PPE) in this type of lab.
flaming them
Loops and needles are sterilized by...
true
Microbes are in the water we drink.
false
Microorganisms are normally found in a person's cerebrospinal fluid. (true or false)
acidophiles
Most fungi and yeast are...
semisolid agar
O/F carbohydrate tubes contain a/an ______. This helps create the anaerobic environment in the F (fermentation) tube necessary for fermentation reactions.
both tubes turn yellow
OF carbohydrate tubes indicate a fermentation reaction when...
diaphragm
On a microscope, the amount of light that reaches the slide is controlled by a _________ located inside the condenser.
hyperthermophiles
Organisms that grow best at temperatures above 80C.
pyschrophiles
Organisms that grow best in a temperature range between -5C and 20C.
thermophiles
Organisms that grow best in a temperature range between 50C and 80C.
strict aerobes
Pseudomonas, Micrococcus, and many Bacillus are ______.
hydrogen peroxide
Reagent used to test for catalase enzyme.
iodine
Reagent used with starch hydrolysis.
pink
Resazurin, an indicator dye, in fluid thioglycollate (FTM), turns ____ in the presence of oxygen.
methylene blue
Simple staining uses
aseptic technique
Taking precautions to prevent the contamination of others, yourself, your surroundings, and your lab supplies is known as...
discard cart
Test tubes are disposed of in/on the....
false
The KOH test is a replacement for the Gram stain technique. (True or false)
false
The catalase test determines the presence or absence of cytochrome oxidase in you organism. (true or false)
beta
The complete lysis of red blood cells around a colony of bacteria.
red
The pH indicator used for the methyl red test turns ____ if acids are present.
blood plasma
The reagent used in the coagulase test is
air
The reagent used in the oxidase test is unstable and can oxidize if exposed to ____ for prolonged periods of time leading to false positive reaction.
facultative anerobe
Escherichia coli is an example of a/an ______.
true
Glass and plastic can block UV radiation. (true or false)
sba plate
Hemolytic capability of an organism can be demonstrated on...
indole, methyl red, voges Proskauer, citrate
IMViC is an acronym for tests that are VERY IMPORTANT when trying to identify organisms like E. coli, Salmonella, Proteus, Klebsiella, etc. What are the tests that IMViC represents?
yellow
If an organism can ferment the carbohydrate in a phenol red carbohydrate tube, the liquid in the tube will turn....
green to blue
If an organism can utilize citrate as a sole carbon source and ammonium salt as a sole nitrogen source, the citrate medium will turn...
methyl red test
If an organism produces butanediol and is positive for the Voges-Proskauer (VP) test, it USUALLY tests negative for the ____ test.
acid
In a phenol red carbohydrate tube, the presence of ____ is indicated by a color change of the pH indicator phenol red.
