AP Biology Unit 4 Test
The following questions refer to an experiment that is set up to determine the relative volume of O2 consumed by germinating and nongerminating (dry) pea seeds at two different temperatures. The change in volume is detected by using a respirometer over a given period of time. The data are given below. The rate of oxygen consumption in germinating pea seeds at 26ºC is
0.05 mL / min
Pyruvate dehydrogenase is an enzyme that converts pyruvate to acetyl-CoA. Acetyl-CoA is further metabolized in the Krebs cycle. A researcher measured the accumulation of acetyl-CoA in a reaction containing pyruvate and pyruvate dehydrogenase under several different conditions (Figure 1). The maximum production rate of acetyl-CoA under condition 1 is closest to which of the following?
1 micromole/sec
Certain chemicals, including sodium fluoride (NaF), are capable of inhibiting specific steps of glycolysis. Figure 1 shows the steps of the glycolysis pathway, indicating where various macromolecules enter the pathway as well as the specific reaction inhibited by NaF. Based on Figure 1, the net number of ATP molecules produced during glycolysis from the metabolism of a single glucose molecule is closest to which of the following?
2
Certain chemicals, including sodium fluoride (NaF), are capable of inhibiting specific steps of glycolysis. Figure 1 shows the steps of the glycolysis pathway, indicating where various macromolecules enter the pathway as well as the specific reaction inhibited by NaF. If NaF is added to cells undergoing cellular respiration, which of the following will most likely accumulate in the cells?
2-phosphoglycerate
A scientist determined the rate of an enzyme-catalyzed reaction by measuring the amount of product formed over time. The following curve was generated from the data collected. During which time interval is the reaction rate lowest?
4-5 minutes
(A) Glysolysis(B) Krebs cycle (citric acid cycle)(C) Calvin cycle (light-independent reactions of photosynthesis)(D) Light-dependent reactions of photosynthesis(E) Chemiosmosis. Process in which sugar is oxidized to pyruvic acid
A
Which of the following describes a metabolic consequence of a shortage of oxygen in muscle cells?
A buildup of lactic acid in the muscle tissue due to fermentation
A scientist determined the rate of an enzyme-catalyzed reaction by measuring the amount of product formed over time. The following curve was generated from the data collected. What is the most likely explanation for the change in the slope of the line between 3 and 5 minutes?
A large amount of the substrate had been consumed.
An experiment to measure the rate of respiration in crickets and mice at 10o C and 25o C was performed using a respirometer, an apparatus that measures changes in gas volume. Respiration was measured in mL of O2 consumed per gram of organism over several five-minute trials, and the following data were obtained. During aerobic cellular respiration, oxygen gas is consumed at the same rate as carbon dioxide gas is produced. In order to provide accurate volumetric measurements of oxygen gas consumption, the experimental setup should include which of the following?
A substance that removes carbon dioxide gas
Gelatin is a protein that is derived from collagen which is found in the bones, skin, and connective tissue of animals. To investigate the ability of various enzymes to digest gelatin, a group of students set up an assay involving camera film. Camera film contains gelatin and appears black when exposed to light but turns clear as the gelatin gets broken down. The students incubated pieces of exposed camera film in test tubes, each containing one of three different enzyme solutions (trypsin, lipase, or amylase) as indicated in Figure 1. The students recorded the time it took for the enzymes to digest the gelatin in each test tube, turning the film from black to clear. Which of the following would be the most appropriate control for this experiment?
A test tube containing a piece of exposed camera film submerged in water
A tissue culture of vertebrate muscle was provided with a constant excess supply of glucose under anaerobic conditions starting at time zero and the amounts of pyruvic acid and ATP produced were measured. The solid line in the graph above represents the pyruvic acid produced in moles per liter per minute. ATP levels were also found to be highest at points A and C, lowest at B and D. A second culture was set up under the same conditions, except that substance X was added, and the results are indicated by the dotted line. Which of the following best accounts for the shape of the solid line between points A and D?
ATP acted as an allosteric inhibitor on one or more of the enzymes.
A researcher claims that the synthesis of ATP from ADP and inorganic phosphate (Pi) is essential to cellular function. Which of the following statements best helps justify the researcher's claim?
ATP hydrolysis is an energy-releasing reaction that is often coupled with reactions that require an input of energy.
Muscle contraction depends on ATP hydrolysis. During periods of intense exercise, muscle cells rely on the ATP supplied by three metabolic pathways: glycolysis, mitochondrial respiration, and the phosphagen system. Figure 1 shows the rates at which the three metabolic pathways produce ATP following the start of an intense period of exercise. Which of the following correctly uses the data to justify the claim that the phosphagen system is an immediate, short-term source of ATP for muscle cells?
ATP production by the phosphagen system increases and decreases rapidly following the start of the exercise period.
In an experiment, a scientist isolates mitochondria from living cells and suspends them in two different buffered solutions. One solution is maintained at pH 4, while the other solution is maintained at pH 9. The scientist finds that mitochondria in the solution at pH 4 continue to produce ATP but those in the pH 9 solution do not. The results of the experiment can be used as evidence in support of which of the following scientific claims about mitochondrial activity?
ATP production in mitochondria requires a hydrogen ion gradient that favors movement of protons into the mitochondrial matrix.
Pyruvate kinase, a key enzyme in the glycolysis pathway, is inhibited by the amino acid alanine. The ability of alanine to inhibit the enzyme is not affected by increasing the concentration of substrate. Which of the following best explains the mechanism by which alanine inhibits pyruvate kinase activity?
Alanine binds to an allosteric site of the enzyme, changing the shape of the enzyme's active site.
(A) Glysolysis(B) Krebs cycle (citric acid cycle)(C) Calvin cycle (light-independent reactions of photosynthesis)(D) Light-dependent reactions of photosynthesis(E) Chemiosmosis. Process in which CO2, is released as a by-product of oxidation-reduction reactions
B
Certain chemicals, including sodium fluoride (NaF), are capable of inhibiting specific steps of glycolysis. Figure 1 shows the steps of the glycolysis pathway, indicating where various macromolecules enter the pathway as well as the specific reaction inhibited by NaF. Tarui disease is an inherited disorder that is caused by mutations in PFKM, the gene that encodes a subunit of phosphofructokinase, an enzyme in the glycolysis pathway. Individuals with Tarui disease produce little or no functional phosphofructokinase in skeletal muscle cells. Based on Figure 1, which of the following best explains why a low carbohydrate diet is recommended for those with Tarui disease?
Carbohydrate metabolism requires all the reactions of glycolysis, and amino acids and fatty acids do not.
Pyruvate dehydrogenase is an enzyme that converts pyruvate to acetyl-CoA. Acetyl-CoA is further metabolized in the Krebs cycle. A researcher measured the accumulation of acetyl-CoA in a reaction containing pyruvate and pyruvate dehydrogenase under several different conditions (Figure 1). Pyruvate dehydrogenase deficiency is a genetic disease most commonly linked to a mutation in the α-subunit of the mitochondrial enzyme that causes the enzyme to cease functioning. As a result of the mutation, affected individuals build up dangerous amounts of lactic acid. Which of the following best explains the buildup of lactic acid in individuals with the mutation?
Cells undergo fermentation because pyruvate cannot be metabolized to proceed into the Krebs cycle.
Alcohol dehydrogenase (ADH) is an enzyme that aids in the decomposition of ethyl alcohol (C2H5OH) into nontoxic substances. Methyl alcohol acts as a competitive inhibitor of ethyl alcohol by competing for the same active site on ADH. When attached to ADH, methyl alcohol is converted to formaldehyde, which is toxic in the body. Which of the following statements best predicts the effect of increasing the concentration of substrate (ethyl alcohol), while keeping the concentration of the inhibitor (methyl alcohol) constant?
Competitive inhibition will decrease because the proportion of the active sites occupied by substrate will increase.
The figures below illustrate the similarities between ATP synthesis in mitochondria and chloroplasts. The figures can best assist in answering which of the following questions?
Do electron transport chains create a gradient so that ATP synthase can generate ATP molecules?
The diagram below shows energy changes in a specific chemical reaction with and without the addition of an enzyme to the reaction. Which of the following questions can best be answered by the diagram?
Does the addition of an enzyme reduce the activation energy required for a reaction?
(A) Glysolysis(B) Krebs cycle (citric acid cycle)(C) Calvin cycle (light-independent reactions of photosynthesis)(D) Light-dependent reactions of photosynthesis(E) Chemiosmosis. Process found in both photosynthesis and cellular respiration
E
Newborn babies and hibernating animals contain a large amount of brown adipose (fat) tissue (BAT). Certain proteins in the BAT cells increase the permeability of the inner mitochondrial membrane to protons, disrupting the proton gradient. Which of the following best predicts the effect of disrupting the proton gradient in BAT?
Electron transport and oxidative phosphorylation will be decoupled, generating more heat but less ATP.
This group of questions refers to molecules of the following substances. (A) Cytochrome (B) FADH2 (C) NAD+ (D) NADP+ (E) Oxygen (O2). Coenzyme that transfers electrons from the Krebs cycle to the mitochondrial electron-transport chain at a lower energy level than that of electrons entering at the beginning of the chain
FADH2
A tissue culture of vertebrate muscle was provided with a constant excess supply of glucose under anaerobic conditions starting at time zero and the amounts of pyruvic acid and ATP produced were measured. The solid line in the graph above represents the pyruvic acid produced in moles per liter per minute. ATP levels were also found to be highest at points A and C, lowest at B and D. A second culture was set up under the same conditions, except that substance X was added, and the results are indicated by the dotted line. Which of the following is most likely to result if oxygen is added to the tissue culture?
For each glucose molecule consumed, more ATP will be formed.
The enzyme hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, which is an important step in glycolysis. The reaction involves the transfer of a phosphate group from ATP to glucose. Either a glucose molecule or a water molecule can fit in the active site of hexokinase. The presence of a water molecule in hexokinase's active site would result in the hydrolysis of ATP to ADP instead of the conversion of glucose to glucose-6-phosphate. Which of the following statements best helps explain the reaction specificity of hexokinase?
Glucose has the right shape and charge to cause hexokinase to undergo a structural change needed for catalysis, whereas water does not.
Which metabolic process is common to both aerobic cellular respiration and alcoholic fermentation?
Glycolysis
Phosphofructokinase (PFK) is an enzyme that catalyzes the conversion of fructose 6-phosphate to fructose 1,6-bisphosphate during glycolysis, as represented in Figure 1. PFK can be allosterically inhibited by ATP at high concentrations. Which of the following is the benefit of regulating glycolysis by the concentration of ATP?
Glycolysis proceeds when the intracellular concentration of ATP is low, which provides ATP to drive cellular reactions.
Certain chemicals, including sodium fluoride (NaF), are capable of inhibiting specific steps of glycolysis. Figure 1 shows the steps of the glycolysis pathway, indicating where various macromolecules enter the pathway as well as the specific reaction inhibited by NaF. An increase in the concentration of protons in the cytosol will most likely have which of the following effects on glycolysis?
Glycolytic enzymes will denature as a result of the increased H+ concentration.
Which of the following statements about mitochondrial chemiosmosis is NOT true?
Heat energy is required to establish the electron transport chain.
The following questions refer to an experiment that is set up to determine the relative volume of O2 consumed by germinating and nongerminating (dry) pea seeds at two different temperatures. The change in volume is detected by using a respirometer over a given period of time. The data are given below. Which of the following conclusions is best supported by the data?
In pea seeds an increase in temperature results in an increase in oxygen consumption.
Which of the following questions will best direct an investigation of the mechanism of ATP synthase?
Is the phosphorylation of ADP by ATP synthase dependent on the formation of a proton gradient?
A researcher claims that increasing the environmental temperature by 10°C will double the rate of an enzyme-catalyzed reaction. To test the claim, the researcher designs an experiment that uses a particular enzyme isolated from plants. The design of the experiment is presented in Table 1. For each test tube in the experiment, the researcher will measure the rate of product formation. Which of the following statements best helps justify the inclusion of test tube 5 as a control in the experiment?
It will provide a measurement of product formation in the presence of a denatured enzyme.
Amylase is a protein that catalyzes the conversion of starch to simple sugars. Amylase activity in an aqueous solution can be measured by using iodine as a starch indicator. A solution containing iodine and starch will have a dark-blue color, whereas a solution containing iodine but no starch will have a light-brown color. The color change of an iodine solution from dark blue to light brown can be used to measure the rate at which starch is converted to simple sugars. A student designs an experiment to investigate the effect of environmental pH on amylase function. The design of the experiment is presented in Table 1. Which of the following statements best justifies the inclusion of test tube V as a control in the experiment?
It will show the color change that occurs in the absence of enzyme activity.
A respirometer is a container used to measure the amount of oxygen consumed by an organism. A respirometer was used to determine how environmental temperature affects the uptake of oxygen in one 300-gram rat and one 50-gram mouse. The results of this experiment are shown on the graph below. Which of the following would most adversely affect the validity of this experiment?
Large variation in metabolic rates between individuals of a species
Based on the data in Figure 1, which of the following most likely represents the change in energy that occurs when ATP hydrolysis is coupled with the phosphorylation of a substrate?
Line 1 represents ATP hydrolysis, and line 4 represents phosphorylation of a substrate.
A respirometer is a container used to measure the amount of oxygen consumed by an organism. A respirometer was used to determine how environmental temperature affects the uptake of oxygen in one 300-gram rat and one 50-gram mouse. The results of this experiment are shown on the graph below. Which of the following hypotheses is best supported by the results of this experiment?
Metabolic rate per gram of tissue is higher in smaller mammals.
This group of questions refers to molecules of the following substances. (A) Cytochrome (B) FADH2 (C) NAD+ (D) NADP+ (E) Oxygen (O2). An intermediate electron acceptor for oxidations that occur in both glycolysis and in Krebs cycle reactions
NAD+
Protein digestion in humans is primarily carried out by three enzymes. Pepsin is found in the stomach (pH2), where it aids in the breakdown of large proteins into smaller peptides, while trypsin and chymotrypsin are found in the small intestine (pH8), where they aid in the further breakdown of the proteins into amino acids and dipeptides that can be absorbed into the bloodstream. Graph 1 shows the effect of pH on the activity levels of the three enzymes. Which of the following best predicts how the structure and function of pepsin will change as it enters the small intestine?
Pepsin will change in shape because of the basic environment of the small intestine; therefore, its enzymatic activity will decrease.
Trypsin and pepsin are enzymes that function in different areas of the digestive tract. One functions in the stomach, where the pH is between 1.5 and 3.5, while the other functions in the small intestines, where the pH is between 6 and 8. Based on Figure 1, which of the following best describes where each enzyme functions?
Pepsin works in the stomach because the optimal pH for pepsin is acidic.
Which of the following can be used to determine the rate of enzyme-catalyzed reactions?
Rate of disappearance of the substrate
A researcher claims that different enzymes exhibit maximal function over different pH ranges. To test the claim, the researcher carries out an experiment that includes three different enzymes: pepsin, salivary amylase, and arginase. The results of the experiment are represented in Figure 1. Which of the following actions will provide the most appropriate negative control for the experiment?
Repeating the experiment with denatured enzymes
On average, more ATP can be produced from an NADH molecule than can be produced from a molecule of FADH2. Based on Figure 1, which of the following best explains the difference in ATP production between these two molecules?
The electrons of FADH2 are transferred through three complexes of the electron transport chain whereas those of NADH are transferred through all four complexes.
A researcher proposes a model of an enzyme-catalyzed reaction in which a reactant is converted to a product. The model is based on the idea that the reactant passes through a transition state within the enzyme-substrate complex before the reactant is converted to the product. Which of the following statements best helps explain how the enzyme speeds up the reaction?
The enzyme's active site binds to and stabilizes the transition state, which decreases the activation energy of the reaction.
The figure above shows an organelle typically found in eukaryotic cells. Which of the following best describes the function of the double membrane system of this organelle?
The inner membrane has specialized proteins that create a hydrogen ion concentration gradient between the intermembrane space and the matrix.
An experiment to measure the rate of respiration in crickets and mice at 10o C and 25o C was performed using a respirometer, an apparatus that measures changes in gas volume. Respiration was measured in mL of O2 consumed per gram of organism over several five-minute trials, and the following data were obtained. According to the data, the mice at 10o C demonstrated greater oxygen consumption per gram of tissue than did the mice at 25o C. This is most likely explained by which of the following statements?
The mice at 10o C had a higher rate of ATP production than the mice at 25o C.
Pyruvate dehydrogenase is an enzyme that converts pyruvate to acetyl-CoA. Acetyl-CoA is further metabolized in the Krebs cycle. A researcher measured the accumulation of acetyl-CoA in a reaction containing pyruvate and pyruvate dehydrogenase under several different conditions (Figure 1). Which of the following best describes the cellular location where pyruvate dehydrogenase is most likely active?
The mitochondrial matrix
Figure 1 shows the amount of product produced in an enzyme-catalyzed reaction over five minutes. Which of the following best explains how the rate of the reaction changes over time?
The rate decreases because the ratio of product to substrate increases.
Two nutrient solutions are maintained at the same pH. Actively respiring mitochondria are isolated and placed into each of the two solutions. Oxygen gas is bubbled into one solution. The other solution is depleted of available oxygen. Which of the following best explains why ATP production is greater in the tube with oxygen than in the tube without oxygen?
The rate of proton pumping across the inner mitochondrial membrane is lower in the sample without oxygen.
Which of the following statements best helps explain the reaction specificity of an enzyme?
The shape and charge of the substrates are compatible with the active site of the enzyme.
Which of the following best describes the function of the coenzymes NAD+ and FAD in eukaryotic cellular respiration?
They accept electrons during oxidation-reduction reactions.
A researcher claims that some bacteria contain factors that influence the function of a particular enzyme but other bacteria do not. To test the claim experimentally, the researcher will grow two different bacterial strains in separate liquid cultures and isolate the contents of the cells in each culture. The researcher will add different combinations of cellular contents, substrate, and enzyme to test tubes containing a buffer solution adjusted to the optimal pH of the enzyme and then measure the rate of product formation. The design of the researcher's experiment is presented in Table 1. Which of the following statements best justifies the inclusion of test tubes 3 and 7 in the experiment?
They will show whether the isolated cellular contents have enzymatic activity.
Scientists were interested in testing the effects of rotenone, a broad-spectrum pesticide, on a cell culture. Cell culture A was used as a control, while culture B was treated with rotenone. After a period of time, the scientists measured the concentration of several metabolites in the mitochondria of cells in both cultures. Their results are shown in the table below. Based on the data in the table, which of the following best explains the effects of rotenone on cellular respiration?
Treated cells are not able to break down NADH because certain enzymes of the electron transport chain are inhibited.
A tissue culture of vertebrate muscle was provided with a constant excess supply of glucose under anaerobic conditions starting at time zero and the amounts of pyruvic acid and ATP produced were measured. The solid line in the graph above represents the pyruvic acid produced in moles per liter per minute. ATP levels were also found to be highest at points A and C, lowest at B and D. A second culture was set up under the same conditions, except that substance X was added, and the results are indicated by the dotted line. It is most reasonable to hypothesize that, in the breakdown of glucose, substance X is
an inhibitor
The reactions of glycolysis occur in the
cytosol
According to the chemiosmotic theory (chemiosmotic coupling), the energy required to move protons from the mitochondrial matrix to the intermembrane space against a concentration gradient comes most directly from
electrons flowing along the electron transport chain
ATP serves as a common energy source for organisms because
its energy can be easily transferred to do cellular work
The enzyme peroxidase is found in many organisms. It catalyzes the breakdown of hydrogen peroxide into water and oxygen gas. The rate of peroxidase activity at different pH values was assessed by students in the lab. The students' results are shown in graph 1. If the experiment is repeated at pH 11, the observed activity level of the enzyme will most likely be
lower than the level at pH 9
Within the cell, many chemical reactions that, by themselves, require energy input (have a positive free-energy change) can occur because the reactions
may be coupled to the hydrolysis of ATP
A scientist determined the rate of an enzyme-catalyzed reaction by measuring the amount of product formed over time. The following curve was generated from the data collected. The rate of the reaction could also be determined by
measuring the change in the amount of substrate
Oxygen consumption can be used as a measure of metabolic rate because oxygen is
necessary for ATP synthesis by oxidative phosphorylation
During respiration, most ATP is formed as a direct result of the net movement of
protons down a concentration gradient
When hydrogen ions are pumped out of the mitochondrial matrix, across the inner mitochondrial membrane, and into the space between the inner and outer membranes, the result is
the creation of a proton gradient
