ASCP Histology Exam Section 8

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The term for .000001 meters is a/an:

Micron

It is necessary to determine if there are any free aldehyde groups present in the tissue sections to be stained with the PAS technique. The best method for determining this is to run a section:

Omitting the oxidizer

What is the chemical reaction called that results in the removal of electrons from a molecule?

Oxidation

In standard paraffin processing, clearing reagents MUST be miscible with which of the following?

Both Alcohols and Paraffins

All of the following are characteristics of formaldehyde EXCEPT:

Expensive and unstable reagent.

Which of the following is the LEAST likely route of entry for the dye powders of hematoxylin or Congo red?

Skin Absorption

What should the histotechnician have done to correct for air bubbles trapped during embedding?

Tap the block with forceps prior to placing on the cold plate.

Muller-Mowry Colloidal Iron stain procedure is a classic example of Prussian Blue reaction.

True

The best overall method to demonstrate enzymes in tissue sections is:

Unfixed frozen

Microscopic evaluation of an H&E stained section of a Zenker-fluid section of lymph node shows a dark brown pigment lying on top of the section. The sections were treated with iodine and sodium thiosulfate prior to staining. One possible cause of the artifact could be that the:

Zenker solution was not washed out before processing.


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