Ch 23 book Q's

The identification of a stop codon for a particular gene is an ex of a. seq recognition b. pattern recognition c. both a & b d. none of the above

a. seq recognition

The technique of tandem mass spec is used to determine a. the amino acid seq of a peptide fragment b. the nucleotide seq of a segment of RNA c. the nucleotide seq of a segment of DNA d. the # of genes in a species' genome

a. the amino acid seq of a peptide fragment

The BLAST program begins w/ a particular genetic seq & a. translates it into an amino acid seq b. determines if it contains one or more genes c. identifies homologs w/in a database d. does all of the above

c. identifies homologs w/in a database

Which of the following can be analyzed using a protein microarray? a. protein expression b. protein fxn c. protein-protein interxns d. all of the above

d. all of the above

During 2D-gel electrophoresis, proteins are separated based on a. their net charge at a given pH b. their mass c. their ability to bind to a specific resin d. both a & b

d. both a & b

Homologous genes a. are derived from the same ancestral gene b. are likely to carry out the same or similar functions c. have similar DNA seq d. exhibit all of the above

d. exhibit all of the above

A gene knockout is a gene a. whose function has been inactivated b. that has been transferred to a different species c. that has been moved to a new location in the genome d. that has been eliminated from a species during evolution

a. whose function has been inactivated

To study gene expression from a particular sample of cells, what material is usually hybridized to the DNA microarray? a. fluorescently labeled mRNA b. fluorescently labeled cDNA c. radiolabeled mRNA d. radiolabeled cDNA

b. fluorescently labeled cDNA

A DNA microarray is a slide that is dotted with a. mRNA from a sample of cells b. fluorescently labeled cDNA c. known sequences of DNA d. known cellular proteins

c. known sequences of DNA

The purpose of a ChIP-chip assay is to determine a. the expression levels of particular genes in the genome b. the amount of a specific protein that is made in a given cell type c. the sites in a genome where a particular protein binds d. all of the above

c. the sites in a genome where a particular protein binds

Which of the following is a reason why the proteome of a eukaryotic cell is usually much larger than its genome? a. alternative splicing b. RNA editing c. posttranslational covalent modifications d. all of the above

d. all of the above