Chapter 9 HW QUIZ

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The use of an antibiotic-resistance gene on a plasmid used in genetic engineering makes

Direct selection possible.

In general, how might recombinant DNA technology be used to prevent a genetic disorder caused by a mutation in a single gene?

To insert a desirable gene, remove an undesirable gene, or replace a defective gene with a functioning gene

In the figure, the vector is

A plasmid.

Which of the following attaches the target gene to a desired location?

DNA ligase

RNA interference (RNAi) is a technology used in which therapeutic application of rDNA?

Gene silencing

Why would a recombinant DNA molecule be inserted into a host cell?

It can be copied, transcribed, and translated into a desired protein.

The value of cDNA in recombinant DNA is that

It lacks introns.

The purpose of the Human Genome Project was to

Sequence the nucleotides in human DNA.

Which of the following is an application that uses PCR?

Sequencing a gene, diagnosing a disease, and providing enough DNA for cloning into another organism

What is meant by the term "transformed bacteria"?

The bacterial cell has taken up extracellular DNA

How do restriction enzymes cut DNA sequences?

They cut DNA at sites, called recognition sites, that have specific nucleotide sequences.

What is the end goal of PCR?

To quickly increase the number of copies of a specific DNA sequence

PCR stands for

polymerase chain reaction.


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