Genetics test 3 ch 23

Human Genome Project goals

- to obtain a genetic linkage map of the human genome -to obtain the DNA sequence of the human genome -to analyze the genomes of other model organism -to develop technology to manage large amounts of data

You would like to perform a chromosome walking experiment to determine the location of the gene X relative to gene Y on a chromosome. Select reagents that you will use.

-Genomic library in a cosmid vector -Subcloning reagents -labeling reagent -additional vector

You are performing a chromosome painting experiment to determine the relative positions of four different DNA sequences along a chromosome. When you look at your final computer image, you see blue chromosomes, but only spots for three of the four probes. What are possible reasons for this outcome? Select all that apply.

-Your probe may not bind to this chromosome -You may have forgotten to add the fluorescently labeled molecule that binds to the 4th probe -You may have forgotten to add the 4th probe

Arrange the following in the proper sequence in which they occur during FISH. 1. Denaturation of DNA 2. Addition of DNA probes 3. Fixation of chromosomes

3, 1, 2

The genome size of a certain bacterium is 2 Mb. What is the minimum number of copies of the genome that should be sequenced using shotgun sequencing so that less than 0.5% of the genome is left unsequenced?

6

Which of the following correctly describes a metagenomic approach in the clinic?

A cheek swab by a dentist to determine the types and distribution of oral bacteria in a patient

Which of the following statements regarding the genomes of different organisms is true?

An organism's genome size does not always correlate with the number of genes it possesses.

The smallest number of contigs possible for an organism equals the number of ___________in that organism.

Chromosomes

The DNA from a _________ vector can replicate in a cell like a plasmid or be packaged into a protein coat like a phage.

Cosmid

A collection of clones, where each of the clones contains overlapping sequence is called a ________

Counting

A molecular marker is a gene that shows a phenotype in the organism.

False

FISH probes are fluorescently-labeled and designed to bind only to non-complementary DNA.

False

Map units are the units of distance used in physical mapping

False

The Sanger dideoxy method is an example of a next-generation sequencing technology.

False

The findings of the Human Genome Project are limited to applications in humans.

False

The general strategy of metagenomics is to study a complex mixture of organisms by isolating them and studying them individually in the laboratory.

False

In situ hybridization experiments include all of the following components except

Interphase cells

The term in situ is ________ for ________ .

Latin; in place

The study of all of the proteins expressed by an organism.

Proteomics

When a large insert from one vector is digested by restriction enzymes and then smaller pieces are inserted into multiple vectors, this is called ________

Subcloning

genetic mapping

The experimental process of determining the relative locations of genes or other segments of DNA along individual chromosomes

A restriction fragment length polymorphism is an example of a molecular marker.

True

In situ hybridization is used to detect the physical location of a gene on a chromosome.

True

Molecular markers can vary between individuals.

True

RFLP analysis usually involves Southern blotting

True

Localization of genes using microscopy.

cytogenetic mapping

The study of the expression of all the genes in an organism

functional genomics

Molecular analysis of the whole genomic sequence of an organism.

genomics

Using genetic crosses to determine relative recombination distances.

linkage mapping

The cells used in the FISH technique are first arrested during

metaphase

Using DNA cloning to determine nucleotide sequence distance.

physical mapping