HW 08 - Transcription 1

These bacteria will be unable to identify and tightly bind promoters

If you have a strain of bacteria that produce nonfunctional sigma factor, which step would you expect to be affected? A) These bacteria will be unable to identify and tightly bind promoters B) These bacteria will be unable to synthesize RNA once an open complex is formed C) These bacteria will be unable to convert a closed complex into an open complex D) These bacteria will be unable to assemble RNA polymerase onto the DNA strand

A strand of mRNA is formed with base sequences complementary to those of DNA

In the process of transcription A) A polypeptide is formed as specified by the genes in a chromosome B) A strand of mRNA is formed with base sequences complementary to those of DNA C) mRNA is formed as coded by introns D) The base sequence of DNA is copied into tRNA E) rRNA is specified by exons in DNA

Coding

The sequence of mRNA will be the same as the __________ strand of DNA. A) Template B) Antiparallel C) Coding D) Antisense

Transcribes the gene for large ribosomal RNAs

RNA polymerase I A) Transcribes the gene for large ribosomal RNAs B) None of the above C) Transcribes the genes for tRNA, ssRNA, and other small RNAs D) Transcribes genes that encode proteins

Acts to position RNA polymerase II for transcription initiation The TATA box is found in many genes transcribed by RNA polymerase II. It is usually centered about -25. If the position of the TATA box is moved, the start site for transcription is also moved

The TATA box A) Serves as a promoter sequence for genes transcribed by RNA polymerase III B) Acts to position RNA polymerase II for transcription initiation C) Is present in all eukaryotic genes D) Is located approximately 100 base pairs upstream of the start site of mRNAs

Requires sigma factor for initiation at specific promoters RNA polymerase holoenzyme (core enzyme plus sigma factor) is required for sequence specific binding at promoters and transcription initiation

The core enzyme of E. coli RNA polymerase A) Synthesizes RNA in the 3' to 5' direction B) Can initiate transcription at specific promoters C) Requires sigma factor for initiation at specific promoters D) Binds DNA in a sequence specific manner but cannot initiate transcription

General transcription factor TFIID For genes transcribed by RNAP II, the initial contact with promoter is made by the general transcription factor TFIID (complex of TBD and at least 12 TBP-associated factors or TAFs)

The first protein complex to bind to the core promoter for a protein coding gene in eukaryotes is A) General transcription factor TFIID B) General transcription factor TFIIB C) General transcription factor TFIIE D) RNA polymerase II

Carboxyl terminal heptad repeats The carboxy terminal domain (CTD) of the largest subunit of RNAPII is composed of 26-52 repeats of a conserved heptad. Phosphorylation of the CTD has several roles in transcription including the transition from initiation to elongation and capping of the 5' end of transcripts

This unique feature of Rpb1 of RNA pol II is necessary for viability and has several roles in transcription. A) Carboxyl terminal heptad repeats B) Alpha helices C) TATA binding protein D) Sigma factor

Nucleotide polymerization occurs in the 5' to 3' direction

Transcription is similar to DNA replication in that A) The same enzyme is used for transcription and to synthesize RNA primers during DNA replication B) The newly synthesized RNA remains paired to the template DNA C) An RNA transcript is synthesized discontinuously and then pieces are then joined together D) Nucleotide polymerization occurs int eh 5' to 3' direction

Transcription initiation Unless gene is transcribed, downstream events cannot occur

What is the most important control point for regulation of genome expression? A) Transcription initiation B) Degradation of proteins and RNA molecules C) Translation initiation D) Processing of transcripts

RNA synthesis is always in the 5'-3' direction

Which of the following is true of RNA synthesis (transcription)? A) RNA synthesis is always in the 5'-3' direction B) RNA polymerase needs a primer to initiate transcription C) New nucleotides are added on to the 2' OH of the ribose sugar D) In transcription, U is inserted opposite T

Transcription starts at the DNA template corresponding to the 3' end of the mRNA Transcription starts at the DNA template corresponding to the 5' end of the mRNA and usually extends beyond the complement of the 3' end of the mature mRNA

Which of the following statements regarding transcription is FALSE? A) Transcription starts at the DNA template corresponding to the 3' end of the mRNA B) Transcription starts at the 3' end of the template DNA C) Transcription occurs in the 5' to 3' direction of the RNA D) Transcription starts at the DNA template corresponding to the 5' end of the mRNA

Sigma subunit The beta and beta prime subunits together make up the catalytic center. The alpha subunit is required for assembly of the core enzyme. The sigma subunit is concerned specifically with promoter recognition

Which subunit of bacterial RNA polymerase makes specific contacts with the -35 and -10 regions of promoters? A) Beta subunit B) Sigma subunity C) Alpha subunity D) Beta prime subunit

TBP TATA binding protein (TBP) has a role in transcription initiation from all three eukaryotic RNA polymerases

Which transcription factor has a role in initiation of transcription from all three eukaryotic polymerases? A) TFIID B) TFIIB C) UBF1 D) TBP

TFIIH TFIIH has several activities including a ATP-dependent helicase activity, kinase activity. It is involved in both initiating transcription and repairing DNA

__________ is involved in both initiating transcription and repairing DNA damage A) TFIIB B) TFIIE C) TFIID D) TFIIH