Its a BIG ONE: Acinetobacter baumanii, Spingomonas paucimobilis, Stenotrophomonas maltophilia Vibrio and Aeromonas Pseudomonas, Burkholderia, Alcaligenes, Achromobacter Campylobacter and Helicobacter.

Optimum growth of Campylobacter jejuni is obtained on suitable media incubated at 42C in an atmosphere containing:

6% O2, 10-15% CO2, 85-90% N2

A patient with a nosocomial pneumonia has a sputum Gram stain that shows many neutrophils and numerous small gram negative coccobacilli. The organism grew in 24 hours as a mucoid, hemolytic colony on blood agar and a colorless colony on MacConkey agar. The organism had the following characteristics: oxidase: negative catalase: positive nitrate: negative ONPG: negative ornithine decarboxylase: negative lysine decarboxylase: negative The organism is:

Acinetobacter baumanii

Which organism is life-threatening in cystic fibrosis patients?

Acinetobacter baumanii

Which organism is seen as a nosocomial infection during the warm months, especially in ICU patients?

Acinetobacter baumanii

Which organism are plump gram negative coccobacilli that resist decolorization and may be mistaken for Neisseria sp.?

Acinetobacter sp.

While swimming in a lake near his home, a young boy cut his foot, and an infection developed. The culture grew a nonfastidious gram negative, oxidase positive, beta hemolytic, motile bacillus that produced deoxyribonuclease. The most likely identification is:

Aeromonas hydrophila

A Campylobacter species isolated from a stool culture gives the following biochemical reactions: naladixic acid: susceptible cephalothin: resistant hippurate hydrolysis: positive oxidase: positive catalase: positive This biochemical profile is consistent with:

Campylobacter jejuni

Infection with which of the following organisms is associated with high mortality rates in neonates?

Elizabethkingia meningoseptica

A Gram stain of a touch prep from a gastric biopsy shows gram negative bacilli that are slender and curved. The most likely pathogen is:

Helicobacter pylori

Which Pseudomonas sp. grows as dry, wrinkly colonies with an overwhelming smell of dirt?

P. stutzeri

An organism isolated from the surface of a skin burn is found to produce a diffusible green pigment on a blood agar plate. Further studies of the organism would most likely show the organism to be:

Pseudomonas aeruginosa

What is the most commonly encountered gram negative bacillus that is NOT a member of the family Enterobacteriaceae?

Pseudomonas aeruginosa

A patient presents with diarrhea after spending 2 weeks in Haiti after the country's devastation by an earthquake. A stool specimen is collected and inoculated to enrichment broth before subculturing to TCBS. After 48 hour of incubation on TCBS, no growth is identified on the media. What should the laboratory scientist do next?

Report the culture as no growth with a comment that indicates the organism may be viable but nonculturable, and the result does not rule out the presence of an infection.

What makes an organism a halophile?

Requires sodium for growth

A blood culture bottle with macroscopic signs of growth is Gram stained and the technician notes small, curved gram-negative bacilli resembling "gull wings". It is subcultured to blood and chocolate agar and inoculated aerobically and anaerobically. After 24 hours, no growth is apparent. The next step should be to:

Subculture the bottle, and incubate in microaerophilic conditions

A gastroenterologist submits a gastric biopsy from a patient with a peptic ulcer. To obtain presumptive evidence of Helicobacter pylori, a portion of the specimen should be added to which media? And why?

Urea Broth

One of the enterotoxins produced by enterotoxigenic Escherichia coli in traveler's diarrhea is similar to a toxin produced by:

Vibrio cholerae

If present, a characteristic that is helpful in separating Pseudomonas aeruginosa from other members of the Pseduomonas family is:

growth at 42C

Which of the following results is typical of Campylobacter jejuni?

optimal growth at 42C

The primary drug of choice for Stenotrophomonas maltophilia is:

trimethoprim-sulfamethoxazole