Lab 2: Sauerkraut Fermentation
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Are lactic acid bacteria catalase + or -?
1mL
How much liquid (in mL) is added when diluting from one glass tube to another?
0.1mL
How much of diluted juice do you add onto a plate?
15g and 600g
How much salt and cabbage are added into the mason jar?
wait for the juice
Once salt is added to the cabbage, what do you do?
black colonies for coliforms green colonies for E. coli others are colorless
What are the expected results for EMB?
pink coloration
What are you looking for when titrating?
lactic acid bacteria
What bacteria can be found in sauerkraut?
detects catalase by adding H2O2
What does the catalase test do and what does it use?
amount of lactic acid produced
What does the titration volume indicate?
eosine methylene blue agar selective for G- bacteria
What is EMB and what is it used for?
sucrose gelatine detects slime-forming lactics
What is SG agar and what is it used for?
tryptone glucose yeast agar allows growth of most bacteria
What is TGY and what is it used for?
bubbles
What is a positive result for the catalase test?
phenolphthalein
What is the indicator for the titration?
1:10 9mL to 1mL
What is the ratio of juice to water in the first "original dilution," and how much water and juice is added?
glass spreader with flaming alcohol
What method was used to spread the liquid on the plate?
heterofermentative homofermentative
What type of organisms are required for sauerkraut fermentation?
remove CO2
Why do you boil the juice with distilled water?
