Lab Quizes Q/A

What does having a color change in the Mannitol salt agar signify? -Fermentation -Base production -Cellular respiration -Anaerobic respiration

Fermentation

Watches, bracelets, and rings should be removed prior to washing hands. True or False

True

Environmental conditions that affect the efficiency of disinfectants and antiseptics do NOT include which of the following? -Type of microbial population -Temperature -Type of substrate on which the microbes exist -Level of pH

Type of microbial population

optimal growth at pH < 7

acidophile

Causes stage (or objective lens) to move upward or downward

adjustment knob

1) With respect to oxygen requirements, an__can use gaseous oxygen and possesses enzymes to process toxic oxygen products.

aerobe

Before heat fixation, a wet smear of bacterial cells on a slide must first be -air-dried. -blotted dry. -rinsed briefly with water. -stained with a basic dye.

air-dried.

Match the following reagents with their role in the Gram stain procedure. -Decolorizer

alcohol/acetone

optimal growth at pH > 7

alkalinophile

4) The__represent a final group of organisms, whose members lack the required enzymes needed for using oxygen in respiration and may not be able to tolerate any free oxygen in their environment.

anaerobes

The nurse would ________ the wound to rid the injury area from microbes.

antsepticize

________is the appropriate chemical to use to clean the table.

bleach

Concentrates light onto the specimen

condenser

A culture which contains one or more unwanted and often unidentified microbes.

contaminated culture

Match the following reagents with their role in the Gram stain procedure. -Primary stain

crystal violet

Please match the terms to the statements that most accurately describe them to test your understanding of inoculation, media, and culture. 3. To cultivate (verb) or observable growth (noun)

culture

Match the following reagents with their role in the Gram stain procedure. -Counterstain

safranin

UV light has ______ wavelengths than visible light waves, and its peak energy is at a wavelength of ______. -longer; 254nm -shorter; 254nm -longer; 100nm -shorter; 100nm

shorter; 254nm

Which of the following is the most resistant to chemical agents? -Spores -Vegetative cells -Younger cells -Older cells

spores

Platform that supports a microscope slide

stage

A second-level culture where an isolated colony from one culture is taken and transferred into a new medium.

subculture

1. These organisms are likely to inhabit a compost pile that is actively composting.

thermophile

Place the descriptions into the correct boxes to test your understanding of how errors would affect the Gram stain result. You may not be able to view any organisms on the slide.

you did not heat fix the smear correctly

Place the descriptions into the correct boxes to test your understanding of how errors would affect the Gram stain result. Gram-negative organisms might incorrectly stain purple.

you forgot the decolorization step

Place the descriptions into the correct boxes to test your understanding of how errors would affect the Gram stain result. Gram-negative organisms might not be visible.

you forgot to apply safranin

Which of the following are reasons for washing hands before and after lab activities? (Check all that apply) -Hands may be a source of contamination of lab experiments. -Hands may become contaminated with infectious bacteria during lab experiments. -Hands may become stained during lab. -Hands may be contaminated with irritating chemicals during experiments. -Hand washing will sterilize hands.

-Hands may be a source of contamination of lab experiments. -Hands may become contaminated with infectious bacteria during lab experiments. -Hands may become stained during lab. -Hands may be contaminated with irritating chemicals during experiments.

When two adjacent thymine molecules are joined by a covalent bond as a result of exposure to UV light, this structure is referred to as a -Thymine couplet -Thymine dimer -Thymine duplex -Thymine doublet

-Thymine dimer

Match the following reagents with their role in the Gram stain procedure. -Mordant

gram's iodine

3. These organisms might grow in the superheated water near hydrothermal vents in the ocean floor.

hyperthermophile

Please match the terms to the statements that most accurately describe them to test your understanding of inoculation, media, and culture. 1. Process of adding a microbe to a growth material

inoculation

_______ is the appropriate chemical to clean the wound

iodin

Increases or decreases the light intensity

iris diaphragm

Please match the terms to the statements that most accurately describe them to test your understanding of inoculation, media, and culture. 2. The material which provides the nutrients for growth

medium

2. The bacteria that grow in and on the human body are in this group.

mesophile

A culture which contains two or more species which can be easily differentiated.

mixed culture

optimal growth at pH = 7

neutrophile

In which circumstance is food and/or beverage allowed in the laboratory? -if containers are covered -never -if containers are kept out of sight -if containers are sealed

never

After light passes through the specimen, it next enters this lens system

objective lens system

2) Expanding on this classification, an__aerobe cannot grow without oxygen.

obligate

Catalase decomposes hydrogen peroxide in which of the following substances? -Oxygen -Carbon dioxide -Hydrogen -Inorganic salt

oxygen

Which of the following infectious agents is least likely to be damaged by UV exposure? -bacteria -viruses -fungi -protozoa -prions

prions

4. These organisms might be isolated from water pools on the surface of a glacier.

psychrophile

Aseptic techniques are used exclusively in the laboratory environment to prevent contamination. True or False

False

Beverages are allowed in the laboratory as long as the containers are covered. True or False

False

The type of culture most frequently used in the laboratory which contains only a single species of microbe.

pure culture

Sharps container

- a hypodermic needle used to draw blood -a broken microscope slide

Which of the following must be used to perform a CAMP test? (Check all that apply.) -"Unknown" beta hemolytic streptoccus to be tested -Blood agar plate -Bile esculin slant -Bacitracin disk -Staphylococcus aureus culture -Streptococcus pneumoniae culture

-"Unknown" beta hemolytic streptoccus to be tested -Blood agar plate -Staphylococcus aureus culture

What incubation temperature is preferred for the growth of most medically important bacteria? (Check all that apply) -37oC -25oC -45oC -98oF

-37oC -98oF

You are studying a bacterium found in the normal intestinal flora of humans and most other mammals, and are doing an experiment to determine the optimum growth rate of this bacteria. You have inoculated five nutrient broths, and are incubating one broth at each of the following temperatures: 5oC, 25oC, 38oC, 42oC, and 55oC. At which temperature would you predict to see the optimum growth rate? -5oC -25oC -38oC -42oC -55oC

-38oC

Indicate the statements that correctly describe microbial colonies. (Check all that apply) -A microbial colony is often visualized as an opaque spot on the surface of an agar plate or slant. -A microbial colony is a visible accumulation of microbial cells on the surface of an agar plate or slant. -Microbial colonies are readily observed in broth media. -The cells in an isolated colony are clones all descended from one cell deposited on an agar plate or slant at the time of inoculation.

-A microbial colony is often visualized as an opaque spot on the surface of an agar plate or slant. -A microbial colony is a visible accumulation of microbial cells on the surface of an agar plate or slant. -The cells in an isolated colony are clones all descended from one cell deposited on an agar plate or slant at the time of inoculation.

Which of the following may indicate that you have correctly transferred an organism from a broth to a slant? (Check all that apply) -After Gram staining a smear prepared from the slant, all of the cells have similar color and morphology under the microscope. -The slant shows colonies in three different colors. -The slant shows only one color of growth. -The slant appears turbid.

-After Gram staining a smear prepared from the slant, all of the cells have similar color and morphology under the microscope. -The slant shows only one color of growth.

You have a nutrient broth, an agar slant, and an agar plate with bacterial growth in or on each. If you wanted to evaluate the macroscopic appearance of the bacteria growing in or on the medium, which of the cultures would be the best choice? -Broth -Agar slant -Agar plate

-Agar plate

Indicate the sites where microorganisms are likely to be found: (Check all that apply) -Air -Laboratory bench surface -Hands -Surface of sterile agar plate -Computer keyboard -Inside tube of sterile broth

-Air -Laboratory bench surface -Hands -Computer keyboard

Mechanisms of action for disinfectants and antiseptics include which of the following? -Inhibit enzymes -Denature proteins -Oxidation -All of the responses are correct -None of the responses are correct

-All of the responses are correct

You have placed cultures in an anaerobe jar, activated the gas pack, and sealed the jar. Later in the day you check the jar and notice that the methylene blue strip is colorless. What does this indicate? -There is a leak in the seal and oxygen is entering the jar. -An anaerobic environment has been successfully established within the jar. -The chemical reactions in the GasPak have not worked properly and no CO2 has been produced within the jar. -The palladium catalyst must be reactivated.

-An anaerobic environment has been successfully established within the jar.

Indicate which of the following are used for the cultivation of obligate anaerobes. -Candle jar -Anaerobe jar -CO2 incubator -Fluid thioglycollate medium

-Anaerobe jar -Fluid thioglycollate medium

Which of the following may provide opportunities for members of resident microbiota to cause disease -Antibiotic usage with change the balance of normal microbiota -Poorly Functioning immune system -Appropriate personal hygiene -Good nutrition -A break in the skin or mucous membrane barriers

-Antibiotic usage with change the balance of normal microbiota -Poorly Functioning immune system

Please select all of the statements that are true about endospores. -Bacterial endospores are highly resistant to heat, drying and radiation. -Endospores are only found in the environment and are not medically relevant. -Endospores can exist in the environment for indefinite periods of time. -Endospores resist boiling and therefore steam must be used to destroy endospores present in food. -Endospores can be formed by any genus of bacteria.

-Bacterial endospores are highly resistant to heat, drying and radiation. -Endospores can exist in the environment for indefinite periods of time. -Endospores resist boiling and therefore steam must be used to destroy endospores present in food.

When completing a quadrant streak, when do you flame the loop? (Check all that apply.) -Before you pick up a loopful of organisms from the original culture -Before you streak quadrant one -Before you streak quadrant two and three -Before you return the loop to the receptacle

-Before you pick up a loopful of organisms from the original culture -Before you streak quadrant two and three -Before you return the loop to the receptacle

Which of the following would be most useful for differentiating a colony of staphylococcus from a colony of streptococcus? -Hemolysis on blood agar -Coagulase test -DNase test -Catalase test

-Catalase test

Which of the following items may be on the laboratory bench top while doing lab work? (Check all that apply) -Cultures (broth tubes, agar plates, agar slants) -Equipment items specifically needed to complete the lab activity. -Backpack, purse -Pencil or pen -Cell phone

-Cultures (broth tubes, agar plates, agar slants) -Equipment items specifically needed to complete the lab activity. -Pencil or pen

Which are advantages of the quadrant streak plate method when compared to the pour plate method? -It requires fewer materials -It requires less skill. -It is a very quick process. -It does not require the use of heat.

-It requires fewer materials -It is a very quick process.

What advantage does the pour-plate method have over the streak-plate method? -It requires less materials. -It provides isolated colonies. -It requires less skill. -It only requires an inoculating tool and one plate.

-It requires less skill.

Jackie went to the sink at the end of lab to wash her hands. After removing her watch and rings, she turned on the water, wet her hands, added soap and thoroughly rubbed and lathered her hands for 30 seconds, cleaning the backs of her hands, her fingernails, and between her fingers. She then turned off the water, and reached for the paper towels to dry her hands. Identify the correct statement below. -Jackie should have used paper towels to turn off the faucet. -Jackie should not have removed her watch and rings prior to washing her hands. -Jackie should have applied the soap to dry hands, and then added the water for lathering. -Jackie used the correct protocol for washing her hands.

-Jackie should have used paper towels to turn off the faucet.

Please choose the answer that best completes the blanks of this sentence in the correct order. _______ is used to stain the endospore, and _______ is the stain used to stain the vegetative cell in spore staining. -Malachite green; carbolfuchsin -Safranin; malachite green -Methylene blue; safranin -Malachite green; safranin

-Malachite green; safranin

The best source of information regarding the hazards posed by any chemical and specific handling instructions is the -lab partner -GOOGLE -lab manual -Material Safety Data Sheet (MSDS)

-Material Safety Data Sheet (MSDS)

What is heat fixation? -The heating of the inoculation loop after removal of the organisms to be applied to the slide. -Passing the slide through the flame of a burner several times after the smear has completely dried. -The heating of the inoculation loop before removal of the organisms to be applied to the slide. -Heating the slide until it is red-hot to kill the organisms before staining.

-Passing the slide through the flame of a burner several times after the smear has completely dried.

Which of the following are the goals of aseptic technique? (Check all that apply) -Prevent contamination of microbial cultures -Prevent contamination of yourself -Increase the number of different bacteria isolated -Use as many different kinds of media as possible -Prevent the contamination of laboratory surfaces -Increase the speed with which laboratory activities can be completed

-Prevent contamination of microbial cultures -Prevent contamination of yourself -Prevent the contamination of laboratory surfaces

Which of the following is a characteristic of a Resident Microbiota -Resident Microbiota often initiate allergic reactions -Resident Microbiota can compete with and limit growth of harmful bacteria -Resident Microbiota are a major source of nutrients for our bodies -Resident Microbiota typically cause serious infections

-Resident Microbiota can compete with and limit growth of harmful bacteria

Which of the following are genera of gram-positive Staphylococcus cocci frequently found as resident microbiota in humans -Staphylococcus -Streptococcus -Escherichia -Entrococcus -Neisseria

-Staphylococcus -Streptococcus

Select the statements that represent goals in correct smear preparation. -The cells must be heat fixed to the slide to prevent removal when stain is washed off the slide. -It is important to minimize any artifacts or distortion of the cells for accurate viewing after staining. -Smears must be thick in order to easily take up stains. -Organisms should be killed on the inoculating loop before application to the slide. -Smears must be thin to allow for observation of single cells.

-The cells must be heat fixed to the slide to prevent removal when stain is washed off the slide. -It is important to minimize any artifacts or distortion of the cells for accurate viewing after staining. -Smears must be thin to allow for observation of single cells.

Please select all of the statements which are TRUE regarding isolated colonies. -The colony results from a single cell or a cluster of cells multiplying into a visible mass. -The cells within the colony are all the same species. -Isolated colonies form on solid nutrient media. -Isolated colonies form in liquid nutrient media. -Isolated colonies can only be obtained via the streak plate method.

-The colony results from a single cell or a cluster of cells multiplying into a visible mass. -The cells within the colony are all the same species. -Isolated colonies form on solid nutrient media. -Isolated colonies can only be obtained via the streak plate method.

If you transfer bacterial growth from a broth to an agar plate but nothing grows after incubation, what may have occurred? -The loop may have been too hot when making the transfer. -Contaminants may have been introduced into the broth culture. -The mouth of the broth tube may not have been passed through the Bunsen burner flame. -The lid may have been left off the Petri dish for too long during the transfer.

-The loop may have been too hot when making the transfer.

Why is the mouth of a culture tube passed through the Bunsen burner flame after removing and before replacing the cap? -To prevent the entry of ambient air which might introduce contaminants. -To make sure the cap will be easily replaced onto the tube -To sterilize the glass rim of the tube.

-To prevent the entry of ambient air which might introduce contaminants.

Which of the following represent important safety procedures for microbiology students? (Check all that apply) -Always wear open-toed shoes so organisms do not become trapped in shoes. -Wash your hands before and after laboratory procedures. -Disinfect your lab bench at the beginning and end of the lab period. -Only drink a closed container of water while conducting lab procedures. -Take extra care around the flame of a Bunsen burner by tying up hair and keeping it from combustibles.

-Wash your hands before and after laboratory procedures. -Disinfect your lab bench at the beginning and end of the lab period. -Take extra care around the flame of a Bunsen burner by tying up hair and keeping it from combustibles.

How does smear preparation of cells from a liquid medium differ from preparation of cells from a solid medium? -Stain is applied to the cells in the culture tube and then, they are applied to the slide. -Heat fixation is used to evaporate the liquid medium. -A needle is used instead of an inoculating loop. -Water is applied to the slide before emulsifying cells from a solid medium.

-Water is applied to the slide before emulsifying cells from a solid medium.

Acetobacter aceti is a bacterial species involved in the production of acetic acid during vinegar production. Which category would this bacteria be classified within? -neutrophile -acidophile -akalinophile

-acidophile

Partial lysis of the red blood cells surrounding a bacterial colony indicate that the colony is ______. -alpha hemolytic -gamma hemolytic -beta hemolytic -nonhemolytic

-alpha hemolytic

The mechanical stage upper knob causes the stage to move -back and forth. -right and left. -up and down. -All of the answer choices are correct.

-back and forth.

The laboratory work surface should be wiped with disinfectant (Check all that apply) -before beginning laboratory procedures -after completing the lab activities -after a spill of microorganisms -whenever microbes are transferred from one medium to another

-before beginning laboratory procedures -after completing the lab activities -after a spill of microorganisms

Which of the following is a possible mistake when performing a quadrant streak that could lead to no growth beyond the first quadrant? Select all that apply. -beginning the second quadrant streak while the loop is still hot -forgetting to sample from the original culture before beginning the second quadrant streak -forgetting to flame the loop before beginning the second quadrant streak -touching the loop to your bench before beginning the second quadrant streak

-beginning the second quadrant streak while the loop is still hot -forgetting to flame the loop before beginning the second quadrant streak -touching the loop to your bench before beginning the second quadrant streak

Which of the following would be most appropriate for disposal of cultures on agar plates? -biohazard bag or bin -sharps container -normal trash receptacle

-biohazard bag or bin

A ______ is a mound of cells on a solid medium that represents the progeny from one original bacterial cell. -quadrant -streak -colony -culture

-colony

Biohazard bag

-contaminated swab -a plastic petri dish with fungal culture

Gram stain

-differentiates cells based on thickness of peptidoglycan layer -does not require heat to be used in staining process -result is purple and red/pink cells -a chemical is used as a mordant

Acid-fast stain

-differentiates cells with high lipid content in cell wall -important diagnostic tool in mycobacterium infections -uses basic fuchsin, acid-alcohol, and methylene blue

Colonies that do not affect the red blood cells in the surrounding medium are ______. -beta hemolytic -gamma hemolytic -alpha hemolytic -alpha prime hemolytic

-gamma hemolytic

Microorganisms that can grow at salt concentrations as high as 15 - 30% are known as: -halotolerant -halophiles -hypertoniphiles -osmophiles

-halophiles

Although Staphylococcus aureus demonstrates optimal growth in an environment of 1% salt, it is able to tolerate up to 13% salt. S. aureus would be described as: -halophilic -osmophilic -halotolerant -hypertoniphilic

-halotolerant

Endospores

-highly resistant to heat and chemicals -stain with malachite green -require autoclave for destruction -formed when conditions become unfavorable -heat is mordant to facilitate staining of these

How is an inoculating loop or needle sterilized prior to use? -exposure to UV radiation for 15 seconds -dipping into a beaker of boiling water -holding in the hottest part of the Bunsen burner flame until red-hot -soaking in 95% ethyl alcohol for several minutes and allowing to air dry

-holding in the hottest part of the Bunsen burner flame until red-hot

Which of the following are potential hazards which you might encounter in the laboratory? (Check all that apply) -infectious microorganisms -open flames -hot surfaces -corrosive chemicals -sharp tools or broken glass -gamma radiation -magnetic fields

-infectious microorganisms -open flames -hot surfaces -corrosive chemicals -sharp tools or broken glass

The coarse adjustment knob on the microscope -All of the answer choices are correct. -locates the specimen. -locates the focus plane -fine-tunes the focus.

-locates the focus plane

A change in what component of microscopy influences the apparent size of the objects being viewed? -light intensity -contrast -magnification

-magnification

Which of the following items should be disposed of in the sharps container? (Check all that apply) -needles -glass pipettes -broken glass -gloves -agar plates -scalpel blades

-needles -glass pipettes -broken glass -scalpel blades

A bacterial isolate is subcultured to two blood agar plates, one of which is incubated in an anaerobe jar, and the other is incubated on a shelf in the incubator. After incubation, growth is observed on the plate from the anaerobe jar, but there is no growth on the plate incubated on the incubator shelf. Based on these results, how would this organism be categorized? -obligate aerobe -obligate anaerobe -aerotolerant anaerobe -facultative anaerobe

-obligate anaerobe

Where should the label be placed on Petri dishes? -on the edge -on the bottom -whichever location is most convenient -on the lid

-on the bottom

Lab coats, gloves, face masks, and goggles are all examples of -fashion items preferred by biology students -personal protective equipment -items supplied by your college or university

-personal protective equipment

Please choose the answer that best fills in the blanks of this sentence in the correct order. Gram-________ cells stain purple whereas gram-_______ cells stain pink or red when using the Gram stain technique. -negative; positive -positive; negative

-positive; negative

Which of the following methods can be used to generate isolated colonies? Select all that apply. -quadrant streak plate method -pour plate method -dilution series plating -optical density reading -butt inoculation of an agar slant

-quadrant streak plate method -pour plate method -dilution series plating

Container for Autoclaving

-reusable glass pipette -used microscope slides -a glass tube containing bacterial culture

vegetative cells

-stain with safranin -high water content -actively metabolizing

Please choose the answer that best completes the blanks of this sentence in the correct order. The ______ plate method involves spreading an inoculum onto the surface of a plate in a pattern that results in isolated colonies in some areas of the plate after incubation, whereas the ______ plate method involves diluting an inoculum into several melted agar tubes and then adding their contents into plates for solidification and incubation. -streak; pour -spread; pour -pour; streak -streak; spread

-streak; pour

Microorganisms are found in nearly every environment and are said to be ____________________. -contaminated -ubiquitous -omniscient -omnipresent

-ubiquitous

Which items can be discarded in a regular trash can? (Check all that apply) -paper towels used to wipe up bacterial spills -uncontaminated waste paper -wrappers from swabs or from disposable pipetttes -used glass slides

-uncontaminated waste paper -wrappers from swabs or from disposable pipetttes

spore stain

-uses malachite green -distinguishes between active metabolic cells and dormant structures

Place the descriptions into the correct boxes to test your understanding of how errors would affect the Gram stain result. Gram-positive organisms might incorrectly stain pink.

-you forgot the crystal violet step -you forgot the Gram's iodine -you applied the decolorizer for too long

Arrange the following steps of applying immersion oil in the correct sequence.

1) Focus on the specimen using the 40x objective lens. 2) Turn the objectives to half way between the 40x lens and the 100x lens. 3) Apply a drop of immersion oil directly onto area of the slide you were viewing. 4) Rotate the 100x lens over the microscope slide, and use the fine focus adjustment knob to sharpen the image.

Place the steps of the Kirby Bauer method of antimicrobic sensitivity testing in the correct order.

1) Label a sterile Mueller-Hinton agar plate with organisms name and your name. 2) Obtain a dilute suspension of the organism in nutrient broth to use as the inoculum. 3) Using a swab, inoculate the agar plate uniformly by swabbing in three directions. 4) Dispense antibiotic disks onto the inoculated surface using sterile forceps or an automatic dispenser. 5) Tap down disks with sterile forceps. 6) Incubate for 18-24 hours at 35oC. 7) Measure diameters of zones of inhibition and compare zone diameters to interpretive charts and determine if S, I, or R.

Each of the following steps are necessary in preparing and observing a wet mount. Place the steps in the correct order.

1) Obtain a clean slide and cover slip. 2) Using a transfer pipette, obtain a drop of specimen and place onto the center of the slide. 3) Carefully place the cover slip over the drop of specimen. 4) Observe preparation under the 10X objective lens. 5) Observe preparation under the 40X objective lens.

Indicate the correct order of steps as you bring an object into focus under high power.

1) Place slide on stage and secure with stage clips. 2) Turn on the light and center the specimen over the light source. 3) Focus specimen on scanning objective using coarse focus adjustment 4) Rotate to low power objective, then use fine focus to sharpen the image 5) Rotate high power lens into position. 6) Readjust fine focus under high power to produce the sharpest image.

Place these steps in the order in which they will be performed when setting up a disk diffusion assay to test the effectiveness of disinfectants and antiseptics.

1) Using a swab, inoculate a suspension of the bacteria being tested onto an agar plate, spreading the inoculum over the entire surface. 2) Saturate sterile filter paper disks with the test chemical (disinfectant or antiseptic) and place onto the inoculated agar plate.Repeat with each chemical being tested. 3) Incubate inoculated agar plates overnight to promote bacterial growth. 4) Observe plates to determine the presence of a zone of inhibition around each antiseptic or disinfectant disk.

Order the reagents used in the acid-fast staining procedure.

1) carbolfuchsin 2) acid-alcohol 3) methylene blue

Please order the following choices to reflect the appropriate sequence of materials used in the Gram staining procedure.

1) crystal voilet 2) gram's iodine 3) alcohol 4) safranin

Please order the steps of this figure to demonstrate your understanding of the aseptic removal of organisms from a culture tube.

1) inoculating loop is heated until it is red-hot 2) organisms are dispersed in the culture tube 3) cap is removed and the mouth of the tube is flamed 4) a loopful of organisms is removed from the tube 5) tube mouth is flamed again 6) cap is replaced on the culture tube

When the oil immersion (100x) objective is used the total magnification will be

1000x

When the low power (10x) objective is used the total magnification will be

100x

Which objective lens requires oil to be applied? -10x -4x -100x -40x

100x

When the high power (40x) objective is used the total magnification will be

400x

When the scanning (4x) objective is used the total magnification will be

40x

Who and/or what is protected by lab safety protocols? (Check all that apply) -Students doing lab activities -Lab instructors and technical assistants. -Anyone who walks into the lab. -The physical lab space and the equipment housed within the lab.

All Correct -Students doing lab activities -Lab instructors and technical assistants. -Anyone who walks into the lab. -The physical lab space and the equipment housed within the lab.

After inoculating and incubating an agar slant from a pure broth culture of a bacterial species such as E. coli, which of the following would indicate an unsuccessful aseptic transfer? (Check all that apply) -There is no growth on the slant. -There are red, yellow, and white colonies on the slant. -There are colonies of similar morphology on the slant. -There is fungal growth in the original broth culture tube.

All correct -There is no growth on the slant. -There are red, yellow, and white colonies on the slant. -There are colonies of similar morphology on the slant. -There is fungal growth in the original broth culture tube.

Please match the cell types with the statements that most accurately describe them to test your understanding of cell walls 3. Acid-fast cells

Cells which contain a thick layer of mycolic acid or cord factor

Please match the cell types with the statements that most accurately describe them to test your understanding of cell walls. 1. Gram-positive cells

Cells which contain a thick layer of peptidoglycan and teichoic acids

Please match the cell types with the statements that most accurately describe them to test your understanding of cell walls. 2. Gram-negative cells

Cells which contain both an inner and outer membrane as well as a thin layer of peptidoglyan

After a patient leaves the examination room, the nurse should _________ the examining table to clean the table surface.

disinfectant

3) Still other organisms, called__, do not require oxygen but can grow in its presence.

facilitative anaerobes

As the concentration of solutes in a solution increases, the water concentration increases. True or False

false

Possessing the enzyme, catalase, is beneficial to the microbe because the enzyme will decompose alcohol. True or False

false

Selective media will incorporate chemical compounds to produce a characteristic change in the appearance of bacterial growth and or the media surrounding the colonies. True or False

false

The rate of death will always increase with increasing concentration True or False

false

When testing an organism with several different antibiotics, the antibiotic that produces the largest zone is the one that the microorganism is most sensitive to. True or False

false