LabCE Course - Semen Analysis
One hour
A semen sample for semen analysis should generally be received at the testing site within what period of time
Mix the original specimen, centrifuge an aliquot, and evaluate again
A semen sample is received in the laboratory for evaluation of fertility. No sperm are observed upon initial microscopic examination of the sample. How should you proceed
White blood cells (WBCs) Spermatids
"Round cells" in a semen sample
Slightly alkaline
normal semen pH
Pinhead
not a sperm because no chromatin is present
Non-Progressive motility = 2; immotile = 3
How should the sperm motility be categorized in this microscopic field of a semen sample viewed at 400X magnification
False
Liquefaction of a semen specimen always occurs immediately after ejaculation
Number of sperm/mL in sample
Sperm concentration
True
The image represents a microscopic field from a wet mount of a semen sample (100X magnification). Round cells are present in this microscopic field
Sperm count
Total number of sperm in entire ejaculation
200 on each side of the hemocytometer for a total of 400
What is the MINIMUM number of sperm that should be counted to achieve the greatest accuracy when performing a sperm count using an improved Neubauer hemocytometer
30 minutes
What is the optimal time to begin initial microscopic examination of a semen specimen, assuming liquefaction is complete
200
What is the recommended MINIMUM number of spermatozoa that should be counted on each side of the hemocytometer when a manual sperm count is performed using an improved Neubauer hemocytometer
1.5 mL
Which of the following represents the lower reference limit for semen volume
Specimen should be maintained between 20 - 37oC after collection prior to delivery to the laboratory Onsite collection is optimal for obtaining the most accurate results
Which of the following statements are TRUE for semen specimen collection and transport
When a sample of semen is allowed to fall freely from a disposable pipette or glass rod, the semen will fall in small drops with very little trailing thread if viscosity is normal
Which of the following statements is true regarding normal viscosity of semen
Examination of two or three samples is recommended to better evaluate male fertility status. Semen analysis can be used to evaluate the success of vasectomy and vasectomy reversal
Which of the following statements regarding semen analysis are TRUE
Prostate Seminal vesicles Bulbourethral gland
directly involved in the production of semen
15 x 106 spermatozoa per ml
lower reference limit for sperm concentration