Mannitol Salt Agar

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What is the ingredient in Mannitoal Salt agar that inhibits the growth of gram - organisms.

7.5% NaCl (salt)

What is the purpose of Mannitol Salt Agar?

It inhibits the growth of all gram - organisms and nonstaphylococcal gram +.

Is Mannitol Salt Agar a selective or differential media or both?

It is both selective and differential

What ingredient (substrate) in this agar makes it differential?

Mannitol

What color is the indicator above pH 8.4?

Pink

What color is the indicator at pH 7.4 to 8.4?

Red

What differentiates Staphlococcus aureus from the other Staphlococcus genera on the MSA plate?

The plate contains sugar mannitol and the pH indicator is phenal red. The pH S. aureus ferment mannitol.

Define Differential Media

This media allows you to differentiate between 2 colonies of bacteria growing on the same plate.

Define Selective Media

This media suppresses the growth of unwanted organisms and encourages the growth of desired microbes.

What color is the indicator at pH 6.8 or below?

Yellow

What is the pH indicator used in the MSA agar?

phenol red which turns yellow in an acidic environmental.


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