MCQ on Recombinant DNA Technology

1. A recombinant DNA molecule is produced by a) joining of two DNA fragments b) joining of two or more DNA fragments c) both a and b d) joining of two or more DNA fragments originating from different organisms

1. d) joining of two or more DNA fragments originating from different organisms

11. The first successful transformation of rDNA molecule into a bacterium was carried out by a) Nathan, Arber and Smith b) Watson, Crick and Wilkins c) Boyer and Cohen d) Paul Berg

11. c) Boyer and Cohen

12. The plasmid used by Cohen and Boyer for their transformation experiment was a) pSC 101 b) PUC 17 c) pBR 322 d) E.coli plasmids

12. a) pSC 101

13. The mechanism of intake of DNA fragments from the surrounding medium by a cell is called a) transformation b) transduction c) both a and b d) conjugation

13. a) transformation

14. Gene cloning refers to the a) production of large number of copies of the gene being cloned b) production of asexual progeny from a single individual or a cell c) both a and b d) none of these

14. a) production of large number of copies of the gene being cloned

15. Paul Berg's gene splicing experiment created the first rDNA molecule which was a a) a T4 phage fragment incorporated into SV40 vector b) a lambda phage fragment incorporated into SV40 vector c) a T4 phage fragment incorporated into pSC 101 vector d) a lambda phage fragment incorporated into pSC 101 vector

15. b) a lambda phage fragment incorporated into SV40 vector

2. The gene formed by the joining of DNA segments from two different sources are called as a) recombinant gene b) joined gene c) both a and b d) chimaeric gene

2. d) chimaeric gene

3. Which of the following enzyme is used to cut DNA molecule in rDNA technology a) ligase b) phosphatase c) ribonuclease d) restriction enzymes

3. d) restriction enzymes

5. The most important discovery that lead to the development of rDNA technology was a) Double helix model of Watson and Crick b) discovery of restriction enzymes c) discovery of ligase enzyme d) discovery of plasmids

5. b) discovery of restriction enzymes

6. Who discovered restriction enzymes a) Nathan, Arber and Smith in 1970 b) Watson, Crick and Wilkins in 1970 c) Boyer and Cohen in 1975 d) Paul Berg in 1975

6. a) Nathan, Arber and Smith in 1970

7. Who created the first rDNA molecule a) Nathan, Arber and Smith b) Watson, Crick and Wilkins c) Boyer and Cohen d) Paul Berg

7. d) Paul Berg

8. The DNA molecule to which the gene of insert is integrated for cloning is called a) carrier b) transformer c) vector d) none of these

8. c) vector

9. The DNA segment to be cloned is called a) gene segment b) DNA fragment c) DNA insert d) all of these

9. c) DNA insert

4. Restriction enzymes are also called as a) biological scissors b) molecular scalpels c) molecular knives d) all of these

a.) biological scissors

10. Which of the following statements are true regarding rDNA technology a) rDNA technology is used to obtain large number of copies of specific DNA fragments b) rDNA technology is used to obtain large quantities of the protein produced by the concerned gene c) rDNA technology is used to integrate gene of interest into chromosomes where it expresses itself d) all of these

d.) all of these