Microscope Lab Quiz

What's the function of the arm?

To support the microscope

What's the function of the base?

To support the rest of the microscope

What is the first step for using a compound light microscope?

When scanning objective is in place, move the stage up with the coarse adjustment knob

By using the idea of depth of field, how can you tell which hair was above the other?

Whichever hair comes into focus first is on the top because it is closest to the eyepiece/objective

Were both hairs under the microscope in focus under low power?

Yes

The distance above the slide in which the object is in good focus

Depth of field

Which objectives do you use when using the fine adjustment knob?

High power

How did you see the "e" under the microscope?

It was upside down

What's the function of the ocular?

It's closest to the eye, and it magnifies

Which way did the "e" move when you moved the slide to the right?

Left

Which objectives do you use when using the coarse adjustment knob?

Scanning and low power

If you were scanning a slide to find a particular area, which objective would be better to use, and why?

Scanning, because it views the whole slide faster, since it is at the least magnification

What are the 3 objective lenses?

Scanning, low, and high power

What is the total magnification produced when the high-power objective is used?

400X (40X10=400)

What is the magnifying power of the high-power objective?

40X

How is the color distributed when viewed under the microscope (patterns)?

There are many colors that combine to form one color

What is the second step for using a compound light microscope?

Place the slide on the stage, view with the scanning objective and focus with the coarse adjustment knob

The ability to distinguish between two separate parts that are very close together

Resolving power

Which way did the "e" move when you moved the slide to the left?

Right

What 4 systems are there in the compound light microscope?

Optical, Focusing, Lighting, and Structural

What is the total magnification produced when the low-power objective is used?

100X (10X10=100)

What is the magnifying power of the low-power objective?

10X

What is the magnifying power of the ocular lens?

10X

What colors do you see under the microscope?

Pink, yellow, black, and blue

Explain why the colors of the magazine picture looked different when you looked at it under the microscope?

Because all colors are made up of other colors

Why must you center and focus the object in the field of view under low power BEFORE switching to high power?

Because it will be hard to find the object on high power if it wasn't focused on low power

Why should a wet mount have no bubbles? (SPONGEBOB LIKES BUBBLES)

Because it will get in the way of viewing the specimen

Why is only the fine adjustment used for high power?

Because the fine adjustment only focuses the slide slightly, and high power viewing and moving is sensitive

Why weren't both hairs in focus when under the microscope under high power?

Because the microscope can only focus on one hair at a time

What must you do to the object on the slide under LOW power BEFORE switching to high power?

Center and focus the object

What is the fourth step for using a compound light microscope?

Center the specimen, and go to high power and focus ONLY with the fine adjustment knob

What is the third step for using a compound light microscope?

Center the specimen, then go to low power and focus with the coarse adjustment knob

Compare the brightness of the field under low and high power. Which is brighter?

Low power

Is the field of view larger under high power or low power?

Low power

The increase of an object's apparent size by using lenses or mirrors

Magnification

Were both hairs under the microscope in focus under high power?

No

How do you calculate the total magnification of a microscope?

Ocular lens X objective lens

What does the diaphragm control?

The brightness of the slide

How do the effects of the coarse and fine adjustments differ?

The coarse adjustment moves the stage at a greater rate than the fine adjustment

What is the resolving power also known as?

The resolution

Which part of the microscope moves when you turn the coarse adjustment?

The stage

Which part of the microscope moves when you turn the fine adjustment?

The stage

What's the function of the objectives?

They are closest to the specimen, and they magnify

What were the two objectives in this lab?

To become familiar with the parts and operation of a compound microscope, and to learn to prepare and observe wet mounts

What's the function of the body tube?

To connect the ocular lens to the objective lenses

What's the function of the fine adjustment?

To focus the microscope under high power

What's the function of the coarse adjustment?

To focus the microscope under scanning and low power

What's the function of the stage clips?

To hold down the slide, so it doesn't move

What's the function of the nose piece?

To move the objectives so they can used easily

What's the function of the stage?

To place the slide on

What's the function of the mirror or illuminator?

To provide light

What's the function of the diaphragm?

To regulate the amount of light

Which way did the "e" move when you moved the slide forward and backward?

Up and down