Microscope Quiz
0.001 mm = _____ micrometers
1
1 mm = _____micrometers
1000
Low power objective = ________ magnification, ________ ocular lens, so total magnification is ______
10x, 10x, 100x
High power objective = _________ magnification, __________ ocular lens, so total magnification is __________
40x, 10x, 400x
What is the highest magnification that can be obtained with a microscope that has a 10x eyepiece with 10x and 43x objectives? Why?
430 because you can only use 1 objective at once
scanning objective = ______ magnification, _______ ocular lens, so total magnification is _____
4x, 10x, 40x
When viewing an object through the high-power objective, not all of the object may be in focus. Why is that?
As magnification increases, resolution decreases (this is when you use the find adjustment). As field of view decreases, field of view is darker (adjust diaphragm for light) To focus within depth of the field, use the fine focus adjustment
What is the relationship between magnification and the diameter of the field of view?
As magnification increases, the diameter of the field of view decreases.
What happens to an image under the microscope? (think about when you have to draw it)
It flips back and to the right
What is the difference between an image viewed through a microscope and the same image viewed with the naked eye?
The Microscope... -is more detailed -makes images much larger and can magnify them 40, 100, or 400 times -the object moves in the opposite direction of how you move the slide -objects are inverted and reversed
The observation of a specimen under both high and low power could be interfered with by a spot on the surface of which structure?
The eyepiece
What is the eyepiece?
The part you look through (aka ocular)
When measuring the diameter of the field of view, what do you count?
The spaces in between the marks
What does the diaphragm do?
controls the amount of light
When a student uses a compound microscope and switches from low power to high power, the diameter of the field of view will ___________
decrease
A student used the low-power objective of a microscope to view the millimeter markings of a ruler. After changing to the high-power objective, the student would observe...
fewer millimeter markings in the microscope field
To locate a specimen on a prepared slide with a compound microscope, a student should begin with the low-power objective rather than the high-power objective because the....
field of vision is larger under low power than under high power
The coarse adjustment of a compound light microscope should be used to ....
focus the image of a specimen under the low power objective lens
What kind of power is the fine adjustment used for?
high-power focus
A student is observing a temporary wet mount of onion epidermis under the low-power objective of a compound light microscope. Which would be the most appropriate procedure for the student to follow in order to view the wet mount under the high-power objective?
increase the amount of light passing through the specimen
What kind of power is the coarse adjustment used for?
low-power focus
largest field of view= lowest _________
magnification
When asked how to move a specimen to a certain place on the slide, observe what you would do normally (without thinking about what happens to the image) and then do the _________of those directions
opposite
After the revolving nosepiece (thing that has all the objectives) is turned, which part must often be adjusted?
the diaphragm
When a student changes the objective lens of a compound microscope from low power to high power the student should remember that...
the field of view will be darker under the high-power objective than the low-power objective
Which part must be adjusted to enable the viewer to continually observe a paramecium swimming at changing levels in a wet mount under the high power?
the fine adjustment
As a student observes an organism under the high-power objective, the organism swims out of focus. To bring it back into focus, the student should....
turn the fine adjustment
