Multiple Choice Questions on Blotting Techniques

3. Arrange the following in correct order 1. Southern Blotting ----A)Alwine 2. Western blotting ----B)E.M.Southern 3. Northern Blotting----C) A. Jeffrey 4. DNA fingerprinting---- D) Towbin a) 1-A, 2-C, 3-D, 4-B b) 1-B, 2-D, 3-A, 4-C c) 1-B, 2-A, 3-D, 4-C d) 1-B, 2-C, 3-A, 4-C

. b) 1-B, 2-D, 3-A, 4-C

Which of the following technique is most suitable for detecting the presence of a gene product a) Dot blotting b) Southern blotting c) plaque blotting d) Western blotting

. d) Western blotting

Western blotting is the technique for the detection of a) specific DNA in a sample b) specific RNA in a sample c) specific protein in a sample d) specific glycolipid in a sample

1. c) specific protein in a sample

Arrange the following in correct order 1. Southern Blotting ----A)RNA-DNA hybrid 2. Western blotting -----B)DNA-DNA hybrid 3. Northern Blotting-----C)Southern blotting 4. DNA fingerprinting---D)antigen antibody reaction a) 1-A, 2-C, 3-D, 4-B b) 1-B, 2-D, 3-A, 4-C c) 1-B, 2-A, 3-D, 4-C d) 1-B, 2-C, 3-A, 4-C

10. b) 1-B, 2-D, 3-A, 4-C

4. Labelled Antibodies are used to detect a) detect the presence of a particular DNA molecule in Southern blotting b) detect the presence of a particular RNA molecule in Southern blotting c) detect the presence of a particular protein molecule in Southern blotting d) detect the presence of a particular protein molecule in Western blotting

4. d) detect the presence of a particular protein molecule in Western blotting

5. Which of the following technique is suitable for identifying mRNA molecule in a sample a) Western blotting b) Southern blotting c) Northern blotting d) Eastern blotting

5. c) Northern blotting

7. Probe is a a) protein for detecting a specific DNA molecule b) short piece of labelled DNA which are complementary to the nucleic acid strand to be detected c) short piece of labelled DNA or RNA which are complementary to the nucleic acid strand to be detected d) none of these

7. c) short piece of labelled DNA or RNA which are complementary to the nucleic acid strand to be detected

6. Which of the following technique doesn't involve electrophoresis for the separation of biomolecules a) Dot blotting b) Southern blotting c) Northern blotting d) Western blotting

a) Dot blotting

2. Which of the following technique is used in DNA finger printing? a) Western blotting b) Southern blotting c) Northern blotting d) Eastern blotting

b.) Southern blotting

9. Aminobenzyloxymethyl filter paper is commonly used for transfer in a) Western blotting b) Southern blotting c) Northern blotting d) Dot blotting

c) Northern blotting