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Formulate a hypothesis describing the effect of high concentration of product on the rate of the reaction catalyzed by MDH

A high concentration of product will slow the rate of reaction as the reaction will reach equilibrium sooner

Formulate a hypothesis describing the effect of heating the enzyme on the rate of the reaction catalyzed by MDH.

Heating the enzyme will first increase the rate of reaction, but if the heat is too much it will slow the reaction as the enzyme protein begins to denature

Formulate a hypothesis describing the effect of increasing the amount of enzyme on the rate of the reaction catalyzed by MDH

The greater the amount of enzymes the faster the reaction will occur

Given what you know about how enzymes and substrates interact, state the reason for thoroughly mixing the enzyme when it is added.

Thoroughly mixing the enzyme when its added increased the chances of enzymes interacting with the substrate. The more substrate/enzyme interactions the faster the reaction

Formulate a hypothesis describing the effect of different pH conditions on the rate of the reaction catalyzed by MDH.

With a higher pH condition, the reaction will occur faster within that environment

In order to determine reaction rates, at what time intervals will you measure the absorbance of the reactions?

every 15 seconds for 3 minutes