PHYSIO EX 8 - chemical and physical processes of DIGESTION

optical

a spectrophometer measures _________ density

water

hydrolases are enzymes that break down large molecules into smaller subunits through the addition of _________

peptides, amino

if pepsin were digesting an actual protein substrate, the product would be _________ and _________ acids

fatty acids

one of the products of the chemical digestion of lipids is _________ _________

stomach

pepsin would be MOST active in the _________

peptidase

proteins and peptides are formed by joining amino acids with a special type of covalent bond called a peptide bond - which of the following enzymes do you think would be specific for a peptide bond? _________

starch

the reagent IKI tests for the presence of _________

glucose, polysaccharides

cellulose and starch are both polymers of _________ and _________

expected, validate

negative results with the negative controls are _________ and _________ the experiment

reducing

the Benedict's assay tests for the presence of _________ sugar

starch, carbohydrate

the substrate for AMYLASE is _________ and _________

protein, peptides

the substrate for pepsin is _________ and _________

starch

the substrate(s) for amylase is/are _________

fatty acids

what is the product of LIPID hydrolysis? _________ _________

decreases

when fatty acids are liberated by LIPASE, the pH _________

mouth, 7

where and why salivary amylase would be most ACTIVE: salivary amylase would be most active in the _________ because pH ___ is where its peak activity is

stomach

where in the body does protein digestion begin? _________

mouth, pancreas

where would pancreatic lipase be active? _________ and _________

7

which pH is ideal for pancreatic lipase digestion? pH _________

d

which of the following is NOT true of controls? a. with a negative control, a negative result is expected b. with a positive control, a positive result is expected c. a positive result with a negative control could mean that a contamination substance is present d. a negative result with a positive control is required to validate the test

specific

which of the following is TRUE of enzymes and SUBSTRATES? enzymes are _________ about the substrates they can act upon

temperature

which of the following is TRUE of enzymes? their activity can be affected by _________ and pH

maltose, glucose

which of the following is an END product of starch DIGESTION _________ and _________

glucose, maltose

which of the following is/are reducing sugars? _________ and _________

physical

which of the following is/are true of BILE? it works by a _________ process

increased

with MORE enzyme activity, the optical density _________