Success Immunology and Serology
87. A living donor is being sought for a child who requires a kidney transplant. The best odds of finding an MHC-compatible donor occur between the child and: A. A sibling (brother or sister) B. An unrelated individual C. The Child's father D. The child's mother
A. A sibling (brother or sister) Because the human leukocyte antigen system is extremely polymorphic, the odds are greatly against finding an HLA-compatible donor in unrelated individuals. The genes coding for HLA antigens are inherited from one's parents and are expressed co-dominantly. Between an offspring and either parent, there is, statistically, a 25% chance of an HLA match. Between siblings, there is a 50% chance of an HLA match.
9. Which of the following is more likely to be diagnostic of an acute infection? A. A total acute antibody titer of 2 followed by a convalescent titer of 16 B. A total acute antibody titer of 80 C. A total antibody titer of 80 D. An IgG antibody titer of 80
A. A total acute antibody titer of 2 followed by a convalescent titer of 16
21. An example of immune injury due to the deposition of antigen-antibody complexes is: A. Acute glomerulonephritis B. Bee-sting allergy C. Contact dermatitis D. Penicillin allergy
A. Acute glomerulonephritis Acute glomerulonephritis is caused by the presence of a soluble circulating antigen (Ag) that provokes and combines with antibody (Ab). As these Ag-Ab somplexes reach a critical size, they are deposited in the glomerular membranes of the kidney. Upon deposition, an acute inflammatory reaction occurs because of complement activation.
8. HLA antigens are found on: A. All nucleated cells B. Red Blood cells only C. solid tissue only D. White blood cells only
A. All nucleated cells HLA= Human leukocyte antigens
26. In individuals allergic to pollen, hyposensitization protocols may be initiated. These individauals receive injections of : A. Allergen B. Pooled human antisera C. Monoclonal antibody directed against human T cells D. Monoclonal antibody directed against human B ells
A. Allergen
100. A 38 year old woman visited her physician because of fatigue, fever and joint pain (proximal interphalangeal, wrist and knee joints). She also noticed sensitivity to the sun and reported having a rash following recent exposure. the physician noted a rash over her nose and cheeks. Lab results included white blood cell count 5.5x10^9/L (reference range 4.8-10.8x10^9/L) and RBC count of 4.5x10^12/L (reference range 4.0-5.4x10^12/L). Urinalysis results were within reference ranges, except for 4+ protein and 1+ RBCs, 0-3 hyaline casts/lpf and 0-1 RBC cast/lpf on microscopic examination. Which of the following tests would be most helpful in diagnosing this patient's condition? A. Anti-nuclear antibody B. alpha-fetoprotein C. Anti-streptolysin O D. Hepatitis profile
A. Anti-nuclear antibody
35. A major advantage of passive immunization compared to active immunization is that: A. Antibody is available more quickly B Antibody persists for the life of the recipient C. IgM is the predominant antibody class provided D. Oral administration can be used
A. Antibody is available more quickly
39. The type of immunity that follows the injection of an immunogen is termed: A. Artificial active B. Natural active C. Artificial passive D. Innate
A. Artificial active
54. Loss of self-tolerance results in: A. Autoimmune disease B. Graft-versus-host desease C. Immunodeficiency D. Tumors
A. Autoimmune disease
49. IgM antibodies react well in complement fixation (CF) tests. Because of this, CF tests for antibodies should: A. Be positive early in the course of the disease B. Be useful in identifying antibodies responsible for a delayed hypersensitivity reaction C. Be useful in identifying antibodies responsible for anaphylactic reactions D. Detect transplacental antibodies
A. Be positive early in the course of the disease
73. The alternative complement pathway: A. Can be activated by bacterial capsule polysaccharides B. Uses C5b as a C3 convertase C. Bypasses steps C3 through C5 D. Is inactivated by properdin
A. Can be activated by bacterial capsule polysaccharides The alternative pathway for complement activation is a more nonspecific defense mechanism, in that it does not require the presence of antibody for activation. It can be activated by a variety of substances, including complex polysaccharides found in bacterial capsules and cell walls. These materials activate C3 directly. Properdin protein stabilizes some of the active complement proteins and C4b2a is a C3 convertase.
61. The activity of natural killer (NK) cells: A. Does not require previous exposure to an antigen B. Involves phagocytosis and killing of bacteria C. Requires interaction with cytotoxic T cells D. Requires interaction with B cells
A. Does not require previous exposure to an antigen The natural killer cells destroy target cells through an extracellular non-phagocytic mechanism. NK cells are part of the host's innate resistance and therefore do not need previous exposure to an antigen to be active. they also do not need interaction with B or cytotoxic T cells.
1. Color Plate 21 ("Y" shaped, right portion of the "Y" prong), depicts a monomeric immunoglobulin molecule. The portion of the molecule indicated by the dotted red circle and the red arrow is called the: A. Fab fragment B. Fc fragment C. Heavy chain D. Hinge region
A. Fab fragment The carboxy-terminus of the heavy chains constitutes the Fc fagment. The hinge region is the area at the center of the "Y", near the carboxyterminuse of the light chains.
5. Monocytes and macrophages play a major role in the mononuclear phagocytic system. For an antibody-coated antien to be phagocytized, what part of the antibody molecule fits into a receptor on the phagoytic cell? A. Fc region B. Fab region C. Hinge region D. Variable region
A. Fc region
91. B lymphocytes and T lymphocytes are derived from: A. Hematopoietic stem cells B. Macrophages or monocytes C. Mucosa-associated lymphoid tissue D. The fetal liver
A. Hematopoietic stem cells
16. Color plate 22 (center of a dimeric IgA molecule) represents a dimeric IgA molecule. The structure printed in red and indicated by the red arrow is called the: A. J-piece B. Hinge region C. Heavy Chain D. Light Chain
A. J-piece
95. In a chemiluminescent immunologic assay, what is the signal detected? A. Light B. An electric signal C. A purple-colored compound D. A yellow-colored compound
A. Light
79. What is the indicator system used in the complement fixation test? A. Sensitized sheep red blood cells B. Fluorescent-labeled antihuman globulin C. Enzyme-labeled antihuman globulin D. Guinea pig complement
A. Sensitized sheep red blood cells
93. In a competitive radioimmunosorbent test (RIST), what does a high signal suggest? A. The patient sample has a low concentration of IgE B. The patient sample has a low concentration of IgM C. The patient sample has a high concentration of IgE D. the patient sample has a high concentration of total antibody
A. The patient sample has a low concentration of IgE
53. Elevated IgE levels are typically found in A. Type I hypersensitivity reactions B. Type II hypersensitivity reactions C. Type III hypersensitivity reactions D. Type IV hypersensitivity reactions
A. Type I hypersensitivity reactions
32. A molecule found in human serum sometimes used as a tumor marker is: A. alpha-fetoprotein B. HBsAg C. Biotin D. CD1
A. alpha-fetoprotein
80. The isotype of an immunoglobulin antibody A. is defined by the heavy chain B. Is defined as different alleles of the same antibody type (e.g. IgG) C. Is constant for all immunoglobulins of an individual D. Is the variation within the variable region
A. is defined by the heavy chain
42. The agglutination pattern shown in Color Plate 23 was observed while performing an antibody titration. this agglutination pattern is and example of: A. A prezone reaction B. A prozone reaction C. A postzone reaction D. Incomplete complement inactivation
B. A prozone reaction Prozone occurs when an extremely high titer of antibody is present. In the first tubes of the titration, not enough antigen is present to allow for cross-linking an d lattice formation. The antibody effectively blocks all the antigen sites present, so agglutination does not occur. Complement is not involved
4. A lymphokine is: A. A soluble mediator produced by granulocytes and affecting lymphocytes B. A soluble mediator produced by lymphocytes C. A soluble mediator produced by plasma cells D. An antibody that reacts with lymphocytes
B. A soluble mediator produced by lymphocytes
97. An 11 year old female presents with fever, sore throat, lethargy, and tender cervical lymphadenopathy. Relevant findings include splenomegaly and lymphocytosis, with many large reactive (atypical) lymphocytes. A heterophile antibody test was negative. Further laboratory results were as follows: CMV: IgG titer= 20; IgM titer=0 EBV VCA: IgG titer= 0; IgM titer=80 Mono spot: IgG titer= 0; IgM titer=0 What conclusion can be made concerning the diagnosis? A. Acute CMV infection B. Acute EBV infection C. Chronic CMV infection D. Chronic EBV infection
B. Acute EBV infection
31. Diagnosis of group A streptococci (Streptococcus pyogenes) infection is indicated by the presence of: A. Anti-protein A B. Anti-DNase B C. Anti-beta-toxin D. C-reactive protein
B. Anti-DNase B
64. The primary mechanism responsible for pathology in systemic lupus erythematosus is: A. Allergic reaction to foreign molecules B. Antibodies directed against self antigens C. Polycolonal activation of cytotoxic T cells D. Lack of intracellular killing after neutrophil phagocytosis of bacteria
B. Antibodies directed against self antigens
50. Which of the following serologic tests is commonly performed by an immunofluorescence method? A. Anti-HBs B. Antinuclear antibody (ANA) C. Antistreptolysin O (ASO) D. C-reactive protein (CRP)
B. Antinuclear antibody (ANA)
36. The strength with which a multivalent antibody binds a multialent antigen is termed: A. Affinity B. Avidity C. Reactivity D. Valence
B. Avidity
38. After activation of the complement system, leukocytes and macrophages are attracted to the site of complement activation by: A. C1 B. C5a C. C8 D. IgM
B. C5a
89. In patients with human immunodeficiency virus infection, immune status can be monitored by measuring the ratio of: A. CD3+ cells to CD8+ cells B. CD4+ cells to CD8+ cells C. Lymphocytes to monocytes D. T cells to B cells
B. CD4+ cells to CD8+ cells
24. Multiple myeloma is a: A. Lymphoproliferative disease of T cells B. Cancer of plasma cells characterized by increased antibody concentration C. Lymphoproliferative disease resulting in a decrease in antibody production D. Cancer of monocytes characterized by increased kappa and lambda chain synthesis
B. Cancer of plasma cells characterized by increased antibody concentration
7. The HLA complex is located primarily on: A. Chromosome 3 B. Chromosome 6 C. Chromosome 9 D. Chromosome 17
B. Chromosome 6
52. In the enzyme-linked immunosorbent assay (ELISA), the visible reaction is due to a reaction between: A. Enzyme and antibody B. Enzyme and substrate C. Fluorescent dye and antigen D. Latex particles and antibody
B. Enzyme and substrate
45. The veneral disease research laboratory (VDRL) test for syphilis is classified as a(n): A. Agglutination reaction B. Flocculation reaction C. Hemagglutination reaction D. Precipitation reaction
B. Flocculation reaction
78. A serum sample is positive for HBsAg. This results indicates that the person from whom the serum was taken: A. Had a hepatitis B infection in the past but overcame the infection B. Has either active or chronic hepatitis B infection C. Was immunized recently against the hepatitis B virus D. Is no infectious for the hepatitis B virus
B. Has either active or chronic hepatitis B infection If a person had overcome a past infection, he or she would have antibody to the surface antigen (anti-HBs) but not the surface antigen Immunization causes formation of anti-HBs antibody, and the surface antigen would not be present in serum
14. Which class of immunoglobulin possesses delta heavy chains? A. IgA B. IgD C. IgE D. IgG
B. IgD The heavy chains divide human immunoglobulin molecules into separate classes and subclasses. The delta heavy chain corresponds to IgD. The remaining classes IgA, IgE, IgG and IgM correspond to alph, epson, gamma and mu
71. Which of the following statements about the test for C-reactive protein (CRP) is true? A. It correlates with neutrophil phagocytic function B. It is an indicator of ongoing inflammation C. It is diagnostic for rheumatic fever D. Levels decrease during heart disease
B. It is an indicator of ongoing inflammation
63. Which of the following is a characteristic of T cells? A. Synthesize antibody B. Mature in the thymus C. Able to bind unprocessed antigen D. Primarily protect against extracellular parasites
B. Mature in the thymus
84. Rheumatic fever sometimes occurs after group A streptococcal infections. In this condition, an auto immune response attacks the tissue of the heart valves. This phenomenon is an example of: A. Epitope spreading B. Molecular mimicry C. Polyclonal B cell activation d. Preferential activation of T helper cells
B. Molecular mimicry
85. "Superantigens" are toxins produced by some strains of Staphylococcus aureus and group A streptococci and cause damage by: A. Molecular mimicry B. Polyclonal T cell activation C. Lysing white blood cells and platelets D. Lysing red blood cells
B. Polyclonal T cell activation
60. Skin testing is a useful diagnostic tool in a number of disorders, such as tuberculosis. Which of the following statements about skin testing is true? A. A positive test depends on preformed antibody. B. Reactivity to a particular antigen may be transferred from one individual to another by sensitized lymphocytes C. The intensity of the response correlates directly with the clinical activity of the disease D. The maximum response will occur immediately
B. Reactivity to a particular antigen may be transferred from one individual to another by sensitized lymphocytes Skin testing is based upon the presence of T cells sensitized to antigen.
99. A 25- year old male presents to his family physician complaining of fatigue, diarrhea, and wight loss of a few months duration. On physical examination the patient is found to have a fever and abdominal discomfort. Laboratory results indicate a white blood cell count of 14.3x 10^9/L (reference range 4.8-10.8 x10^9/L). Assays for HBSAg and anti-HCV are negative. An ELISA test for antibodies to the human immunodeficiency virus (HIV) performed on the patient's serum is found to be reactive. What step should be taken next? A. Call the physician with the HIV result B. Repeat the HIV ELISA test on the sample C. Test the patient's serum for anti-HBS D. Contact the patient to collect a second sample.
B. Repeat the HIV ELISA test on the sample
33. Which cell is the principal source of interleukin 2? A. B cell B. T cell C. Monocytes D. Plasma cell
B. T cell Interleukin 2 is a lymphokine produced by activated T helper cells
6. Cell-mediated immunity is primarily mediated by: A. B cells B. T helper cells C. Plasma Cells D. Dendritic cells
B. T helper cells The secrete several different lymphokines that stimulate a number of other cells, such as cytotoxic T lymphocytes and monocytes.
92. Contact dermatitis is mediated by: A. B lymphocytes B. T lymphocytes C. Macrophages D. Polymorphonuclear cells
B. T lymphocytes Contact dermatitis is a delayed-type hypersensitivity reaction mediated by T cells. Antibody is not involved in this type of hypersensitivity, so B cells play no role in it. Neither macrophages nor neutrophils are involved in this type of hypersensitivity.
56. Which of the following statements about immunoglobulin light chains is true? A. Each immunoglobulin monomer has either one kappa or one lambda chain B. There are two types: kappa and lambda C. They consist of constant regions only D. They form part of the Fc fragment
B. There are two types: kappa and lambda Light chains are of two distinct types: kappa and lambda. Either type may combine with any of the heavy chains, but in any one molecule, only one type is found. Each immunoglobulin monomer contains two light chains, either kappa or lambda. They extend into the Fab, or antigen-binding, site. This half of the chain is highly variable, whereas the carboxy-terminal portion of the molecule is a constant region.
68. An Ouchterlong gel diffusion plate is depicted in Color Plate 24. The center well contains antibody, and the peripheral wells contain antigens labeled 1 through 4. What is the relationship between the antigens in wells 2 and 4? A. Cannot be determined B. They are identical C. They are unrelated D. They react incompletely with the antibody
B. They are identical When two antigens are identical, a smooth curved line of precipitation is formed between them. In the diagram, the antigen in well 2 is identical to one of two antigens in well 1. The same antigen in well 1 is identical to antigen in well 4. Therefore, it follows that antigens 2 and 4 are identical
75. Incompatible blood transufsions are examples of: A. Type I hypersensitivity reactions B. Type II hypersensitivity reactions C. Type III hypersensitivity reactions D. Type IV hypersensitivity reactions
B. Type II hypersensitivity reactions Incompatible blood transfusions are examples of a type II hypersensitivity reaction. These reactions are characterized as the antigen being a part of a cell. Antibody binds to the antigen, complement is activated, and the red blood cells are lysed.
94. An antibody titration is depicted in COlor Plate 25. In this tiration , a 0.2 mL aliquot of patient's serum sample was addded to 0.8 mL of saline, and this mixture was placed into tube #1. A 0.5 mL sample was removed from tube #1 and placed into tube #2, containing 0.5 mL of saline. This procedure was repeated through tube #10. The dilutions were assayed for antibody to an infectious agent. How should the antibody titer be reported? A. 256 B. 512 C. 640 D. 1280
C. 640
40. The type of immunity that follows the injection of antibodies synthesized by another individual or animal is termed: A. Artificial active B. Natural adaptive C. Artificial passive D. Natural passive
C. Artificial passive
88. Cells that can act as antigen-presenting cells for exogenous antigens include A. All nucleated cells B. Endothelial cells C. B Lymphocytes D. T Lymphocytes
C. B Lymphocytes
65. Which complement protein is present in the greatest concentration in human serum? A. C1 B. C2 C. C3 D. C4
C. C3
96. A 28 year old female complains to her family physician of abdominal pain, loss of appetite, and low-grade fever. Physical examination reveals abdominal tenderness and a low-grade fever. Her physician orders a hepatitis profile and obtains the results below: Anti-HAV- nonreactive Anti-HBc- Reactive Anti-HBs- Nonreactive HBsAg- Reactive HBeAg- Reactive Anti-HCV- Nonreactive Which of the following is the most likely conclusion? A. Acute HAV infection B. Acute HBV infection C. Chronic HBV infection D. Immunity to HBV due to past infection
C. Chronic HBV infection The presence of HBsAg indicates viral replication and that the patient is infectious; this marker can be seen in both acute and chronic infections. The lack of anti-HBs indicates taht the patient is not immune to the infection. The presence of anti-HBc and HBeag with HBsAg indicates a chronic infection
19. When performing the enzyme-muliplied immunoassay technique (EMIT), how is the ligand in the patient's serum detected? A. Agglutinates by binding to antiboyd-coated latex beads B. Binds to enzyme-labeled antibody C. Competes with enzyme-labeled antigen for binding to a specific antibody D. Forms antibody-antigen complex and precipitates
C. Competes with enzyme-labeled antigen for binding to a specific antibody The labeled ligand is designed so that following antibody binding, the enzyme is inactive. As the ligand concentration in the test sample increases, more enzyme-labeled ligand remains unbound, resulting in greater enzyme activity.
12. An improtant part of the nonspecific immune response is (are): A. B cells B. Basophils C. Complement cascade D. Cytotoxic T lymphocytes
C. Complement cascade
44. The rapid plasma reagin assay for syphilis does not need to be read microscopically because the antigen is: A. Cardiolipin B. Complexed with latex C. Complexed with charcoal D. Inactivated bacterial cells
C. Complexed with charcoal
3. Which of the following is characterstic of B cells? A. Phagocytic B. Participate in antibody-dependent cellular cytotoxicity (ADCC) reactions C. Contain surface immunolobulins D. Secrete the C5 component of complement
C. Contain surface immunolobulins
47. The portion of an antigen that binds to an antibody or T cell receptor is called a(n): A. Allergin B. Avidin C. Epitope D. Valence
C. Epitope
62. Interaction between B and T helper cells involves A. MHC II molecule on B cell binding to MHC I molecule on the T cell B. MHC II molecule on B cell binding to CD3 on the T cell C. Foreign antigen on B cell binding to T cell receptor D. CD3 molecule on B cell binding to T cell receptor
C. Foreign antigen on B cell binding to T cell receptor
23. In bone marrow transplantation, immunocompetent cells in the donor marrow may recognize antigens in the recipient and respond to those antigens. This phenomenon is an example of: A. Acute rejection B. Chronic rejecton C. Graft versus host disease D. Hyperacute rejection
C. Graft versus host disease Bone marrow transplants by their nature contain immunologically competent cells: B cells and T cells in particular. Unless the transplanted marrow is HLA-matched perfectly to the donor, the immunocometent cells in the transplant will recognize and react against the non-self HLAs of the recipient's tissues. This phenomenon is known as graft-versus-host disease, because the graft attempts to reject its host.
59. Antibodies that bind to the same epitope are of the same: A. Allotype B. Autotype C. Idiotype D. Isotype
C. Idiotype "Allotype" refers to different alleles of the same isotype. Genetically different individuals will produce antibodies of the same isotype, but they would have a different allotype
17. Which class of immunoglobulin binds to basophils ad mast cells to mediate immediate hypersensitivity reactions? A. IgA B. IgD C. IgE D. IgG
C. IgE
13. The major class of immunoglobulin found in adult human serum is: A. IgA B. IgE C. IgG D. IgM
C. IgG
34. Diagnostic reagents useful for detecting antigen by the coagglutination reaction may be prepared by binding antibody to killed staphylococcal cells via the Fc receptor of staphylococcal protein A. The class of antibody bound by this protein is: A. IgA B. IgD C. IgG D. IgM
C. IgG
20. Severe combined immunodeficiency (CID) is an: A. Immunodeficiency with decreased B cells and neutrophils B. Immunodeficiency with lymphocytopenia and eosinophilia C. Immunodeficiency with decreased or dysfunctional T and B cells D. Immunodeficiency with decreased lymphocytes and decreased complement concentration
C. Immunodeficiency with decreased or dysfunctional T and B cells
29. A kidney transplant from one identical twin to another is an example of a(n): A. Allograft B. Autograft C. Isograft D. Xenograft
C. Isograft Autografts are transplantation from one site to another in the same individual Xenograft is transplantation between different species Allograft is transplantation between two nonidentical individuals of the same species
81. A patient report states the presence of serum antibodies to OspC. What disease does the patient most likely have? A. Syphilis B. Strep throat C. Lyme disease D. Rubella
C. Lyme disease IgM antibody to OspC is an important early marker in the diagnosis of Lyme disease.
74. A cut on a person's finger becomes contaiminated with the bacterium Stephyloccus aureus. The first response by the immune system consists of activity of: A. B cells B. Monocytes C. Neutrophils D. T cells
C. Neutrophils
43. The antibody most frequently present in systemic lupus erythematosus is directed against: A. Surface antigens of bone marrow stem cells B. Surface antigens of renal cells C. Nuclear antigen D. Myelin
C. Nuclear antigen
10. A young woman shows increased susceptibility to pyogenic infection. Upon assay, she show a low level of C3. Which of the following statements is probably true? A. She has an autoimmune disease with continual antigen-antibody activity causing consumption of C3 B. She has DiGeorge syndrome C. She has decreased production of C3 D. She may produce an inactive form of C2, a precursor of C3.
C. She has decreased production of C3 diGeorge syndrome is a deficiency in T cells and complement protein C2 is not a precursor of C3
37. How does the secondary humoral immune response differ from the primary response? A. The lag phase (the time between exposure to immunogen and production of antibody) is longer in the secondary immune response B. IgM is the predominant antibody class produced in the secondary immune response. C. The antibody levels produced are higher in the secondary immune response. D. Cytotoxic T lymphocytes play an important role in the secondary response.
C. The antibody levels produced are higher in the secondary immune response.
2. A hapten is: A. Half of an immunoglobulin molecule B. A carrier molecule for an antigen that is not antigenic alone C. An immunoglobulin functional only in the presence of complement D. A determinant capable of stimulating an immune resonse only when bound to a carrier
D. A determinant capable of stimulating an immune resonse only when bound to a carrier
72. In the classical pathway of complement activation, A. C3 is activated by binding C-reactive protein B. The sequence of activation is C1, C2, C3, C4 C. C1q is activated by the presence of a single Fab region D. Activation by antibody requires one IgM or two IgG molecules
D. Activation by antibody requires one IgM or two IgG molecules Complement attaches to the Fc portion of the antibody molecule. At least two Fc binding sites are required for C1q to attach. Therefore, activation requires two IgG molecules or a single molecule of IgM, Which is a pentamer. The C proteins were named in order of discovery. The correct reaction sequence is C1, C4, C2, C3. As the last step of this reaction sequence, C3 is split into C3a and C3b.
18. Type I hypersensitivity is: A. Associated with complement mediated cell lysis B. Due to immune complex deposition C. Mediated by activated macrophages D. An immediate allergic reaction
D. An immediate allergic reaction
69. Which of the following complement proteins is part of the membrane attack complex? A C1 B. C3 C. C4 D. C5
D. C5
25. Which one of the following decribes a direct immunofluorescence assay? A. Conugated reagent antigen reacts with antibodies to form antigen-antibodycomplexes B. Antigens reacts with unlabeled antibody forming antigen-antbody complexes that attach to labeled antibodies C. A dye is attached to a molecule and it reacts with an immune complex to produce a color D. Conjugated reagent antibody reacts with antigen to form antigen-antibody complexes
D. Conjugated reagent antibody reacts with antigen to form antigen-antibody complexes
51. The Fab portion of an antibody: A. Binds T cell receptor B. Consists of two light chains only C. Consists of two heavy chains only D. Contains the hypervariable reagion
D. Contains the hypervariable reagion
55. A human cell with CD8 on its surface is most likely a: A. B cell B. Monocyte C. T helper cell D. Cytotoxic T cell
D. Cytotoxic T cell The CD8 molecule is found primarily on cytotoxic T lymphocytes. T helper cells possess CD4 on their surface, as do several other cell types. CD3 is a marker found on most T cells.
82. Patient serum is mixed with a suspension of guinea pig antigen. When the sample is then mixed with horse red blood cells, agglutination occurs. This is suggestive of an infection caused by: A. Borrelia burgdorferi B. Hepatitis B virus C. Hepatitis C virus D. Epstein-Barr virus
D. Epstein-Barr virus The Davidsohn differential test can be used to detect heterophile antibodies produced during infectious mononucleosis. These antibodies are not adsorbed by guinea pig antigens. Therefore, the antibodies are available to agglutinate horse red blood cells. Forssman heterophile antibodies are absorbed by guinea pig antigens and would not agglutinate the horse red blood cells.
77. Which of the following is an example of a treponemal antigen test used for the diagnosis of syphilis? A. CRP B. RPR C. VDRL D. FTA-ABS
D. FTA-ABS FTA-ABS=Fluorescent treponemal antibody absorbance VDRL= Venereal Disease Research Laboratory RPR= Rapid Plasma reagin
86. The first serologic marker to appear in patients with acute hepatitis B virus infection is: A. Anti-HB B. Anti-HBc C. Anti-HBe D. HBsAg
D. HBsAg
90. Why does vaccination against hepatitis B virus (HBV) also prevent hepatitis D virus (HDV) infections? A. An immunogen from HBV in the vaccine is also associated iwth HDV. B. The HBV vaccine induces formation of heterophile antibodies that cross react with HDV C. The HBV vaccine stimulates liver cells to produce antiviral molecules active against all hepatitis viruses D. HDV requires the host to be concurrently infected with HBV
D. HDV requires the host to be concurrently infected with HBV
58. Monoclonal antibodies are produced by: A. Cultured T cells B. Human plasma Cells C. Mouse plasma cells D. Hybridomas
D. Hybridomas T cells do not produce antibodies; Hybridomas have been prepared from mouse and human plasma cells fused with myeloma cells.
15. Which class of immunoglobulin possesses 10 antigenic binding sies? A. IgA B. IgD C. IgG D. IgM
D. IgM
22. The serologically detectable antibody produced in rheumatoid arthritis (RA) is primarily of the class: A. IgA B. IgE C. IgG D. IgM
D. IgM
27. After exposure to antigen, the first antibodies that can be detected belong to the class: A. IgA B. IgE C. IgG D. IgM
D. IgM High levels of IgM in a patient is indicative of a recent infection
57. Which of the following statements applies to the Fc fragment of an immunoglobulin molecule? A. It consists of the entire heavy chain B. It contains the variable region of the heavy chain C. It contains the antigen binding sites of the molecule D. It is the region of the molecule that binds to receptors on various white blood cells
D. It is the region of the molecule that binds to receptors on various white blood cells
11. What is the predominant type of antibody found in the serum of neonates born after full-term gestation? A. Infant IgA B. Infant IgG C. Infant IgM D. Maternal IgG
D. Maternal IgG
48. Identical antibodies produced from a single clone of plasma cells describes: A. Reagin B. Cold agglutinins C. Heterophile antibodies D. Monoclonal antibodies
D. Monoclonal antibodies Monoclonal antibodies are derived from a single clone of plasma cells. Plasma cells are fused with a cancerous myeloma cell. Reagin has two meanings: it can refer to the antibody produced during syphilis or it can refer to IgE. Heterophile antibodies are antibodies produced following exposure to an immunogen that are able to bind a similar but different molecule.
41. Innate immunity inclues: A. Anamnestic response B. Antibody production C. Cytotoxic T cell activity D. Phagocytosis by polymorphonuclear cells
D. Phagocytosis by polymorphonuclear cells
76. A soluble antigen and soluble antibody reacting to form an insoluble product describes: A. Agglutination reactions B. Heterophile reactions C. Labeled reactions D. Precipitation reactions
D. Precipitation reactions
46. One cause of a false-positive VDRL test is: A. Brucellosis B. Treponema pallidum infection C. Rocky mountain spotted fever D. Systemic lupus erythematosus
D. Systemic lupus erythematosus Other false positives include rheumatic fever, infectious mononucleosis, malaria and pregnancy
30. In Bruton disease, measurement of serum immunoglobulins would show: A. Elevated levels of IgE B. Elevated levels of IgG C. Normal levels of IgG and IgM but reduced levels of IgA D. The absence of all immunoglobulins
D. The absence of all immunoglobulins
28. Corneal tissue may be transplanted successfully from one patient to anotehr becuase: A. The cornea is nonantigenic B. Corneal antigens do not activate T cells C. Anticorneal antibodies are easily suppressed D. The cornea occupies a privileged site not usually seen by the immune system
D. The cornea occupies a privileged site not usually seen by the immune system Corneas are readily transplanted from one individual to another. This is because the cornea is nonvascularized and is a sequestered site. Thus the immune system of the host does not "see" the cornea and recognize it as foreign
83. Hashimoto disease is an autoimmune disease primarily involving the: A. Kidneys B. Liver C. Lungs D. Thyroid gland
D. Thyroid gland
66. An autoimmune disease causing destruction of pancreatic cells can result in: A. Hashimoto disease B. Multiple sclerosis C. Myasthemia gravis D. Type I diabetes
D. Type I diabetes Destruction of the beta cells in the pancreas results in type 1 diabetes. An autoimmune response destroys the insulin-producing cells. The immune response is probably due to molecular mimicry. Cytotoxic T cells and antibodies directed against an infectious agent cross react to the beta cells
70. Which of the following is characteristic of contact hypersensitivity reactions? A. Caused by preformed IgE antibody B. Characterized by infiltration of neutrophils into the area of reaction C. The primary symptoms often occur in the respiratory tract. D. Usually due to a hapten
D. Usually due to a hapten Contact dermatitis is a cell-mediated hypersensitivity reaction. The offending substance is typically a hapten that combines with a carrier molecule on the skin surface. the hapten-carrier complex is recognized by T cells. IgE mediates immediate hypersensitivity reactions such as hay fever and some forms of asthma
98. A male infant had been well until about 5 months of age, at which time he was diagnosed as having otitis media and bronchitis caused by Haemophilus influenzae. Over the next serval months he presented with stroptococcal pneumonia several times. At 10 months of age a serum protein electrophoresis showed a virtual lack of gamma globulins. Quantitative serum levels were as follows: 75 mg/dL IgG and undetectable levels of IgM, IgA and IgE. there were a normal number of T cells, and they exhibited normal mitogen stimulation. What disease does this child most likely suffer from? A. Combined immunodeficiency B. DiGeorge syndrome C. Iatrogenic immunodeficiency D. X-linked agammaglobulinemia
D. X-linked agammaglobulinemia
67. An Ouchterlong gel diffusion plate is depicted in Color Plate 24. The center well contains antibody and the peripheral wells contain antigens labeled 1 through 4. What is the relationship between the antigens in wells 2 and 3? A. 2 is part of 3. B. 3 is part of 2. C. they are identical D. they are unrelated
D. they are unrelated The two antigens are not related. There are two different antibodies that are able to react with the two antigens, forming precipitin lines that cross. If the antigens were identical, a smooth curve precipitation line would have formed.
