14.3-14.5

Which component is not involved during the formation of the replication fork?

Ligase

how did the scientific community learned that DNA replication takes place in a semi conservative fashion?

Mendelsons experiments with E. coli grown in N 15 deduced his finding

Mendelson and Stahls experiment prove that DNA replicates by which mode?

semi conservative

what are Okazaki fragments and how are they formed?

short DNA fragments are formed on the lagging strand synthesized in a direction away from the replication fork. These are synthesized by DNA polymerase

The ends of the linear chromosomes are maintained by...

telomerase

How do the linear chromosomes in eukaryotes ensure that it ends are replicated completely?

telomerase has an inbuilt RNA template that extends the three prime end, so primer is synthesized and extended. Thus ends are protected

Explain the events taking place at the replication fork. If the gene for Gila case is mutated, what part of replication will be affected?

at the replication fork, the events taking place are helicase action, binding of a single-stranded binding proteins, primer synthesis, and synthesis of new strands. If there is a mutated helicase gene, the replication fork will not be extended.

What is the role of a primer in DNA replication, what would happen if you forgot to add a primer in a tube containing the reaction mix for a DNA sequencing reaction?

primer provides a three prime ohage group for DNA polymerase to start adding nucleotides. There would be no reaction in the two without a primer and no vans would be visible on the electrophoresis

DNA replication is by directional and discontinuous, explain your understanding of those concepts.

At the origin of replication, to what replication forks are formed that are extended into directions. On the lagging strand Okazaki fragments are formed in a discontinuous manner. On the leading strand replication continues in forward direction

You isolate yourself strain in which the joining together of the Okazaki fragments is impaired and a suspect that mutation has occurred in an enzyme found at the replication fork. Which enzyme is most likely to be mutated

DNA ligase, as this enzyme joins together okazaki fragments

If the rate of replication in a particular prokaryote is 900 nucleotides per second, how long would it take for 1.2 million base pair genomes to make 2 copies?

1333 seconds or 22.2 minutes

if the sequence of the 5'-3' strand is AATGCTAC, then the complementary sequence has the following sequence:

3'-TTACGATG-5'

Which direction does DNA replication take place?

5'-3'

Which of the following does the enzyme primates synthesize?

RNA primer