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What is the absolute lymphocyte count if 46% lymphocytes are counted in the differential count of a blood specimen with a total WBC count of 11.5x10 exposan3/uL?
11.5x.46=5.29x10exposan3/uL
How many platelets should be note per oil-immersion field on a smear if the platelet count is normal?
7 to 20 platelets will indicates a normal platelet count
What could cause the number of band cells to be increased above normal?
Bacterial infection
Describe the area of the smear in which the differential count should be performed.
perform the count in an area in the feathered edge of the smear where the red cells are just touching but not overlapping
Functions of Lymphocytes
produce antibodies and messenger molecules (cytokines)
Hypochromic RBCs
they have less than the normal amount of hemoglobin; a narrow ring of hemoglobin is visible around the outer edge of the cell, and a large central area of pallor is present.
Normochromic RBCs
they stain evenly, with only a small pale area in the center of the cells.
Why is it important to correctly identify blood cells?
to identify any abnormalities present; this information can be important in diagnosis and treatment of certain diseases. such as anemia, leukemias, other blood diseases, as well as diseases and conditions that arise in other parts of the body, such as infections
What is the purpose of a WBC differential count?
The purpose of the count is to classify WBC's into different types and determine the relative frequency of each WBC type in the peripheral blood.
What are the functions of Neutrophils?
Fight bacterial infections and are active in phagocytosis
Describe the appearance of atypical lymphocytes and name one condition that causes them.
Most atypical lymphocytes are larger than small lymphocytes and are characterized by a large amount of blue cytoplasm easily indented by RBCs. it causes by the lymphocytes to have a holly-leaf appearance. reactive lymphocyte or atypical lymphocyte
List the five types of WBCs, and state the adult reference ranges in percentages.
Neutrophils, Eosinophils, Basophils, Lymphocytes, Monocytes
What three characteristics of WBCs are evaluated in cell identification?
Relative cell size, Nuclear characteristics, cytoplasmic characteristics
what safety hazard are associated with performing the WBC differential count?
Stained blood smears do not represent a biological hazard. However, physical hazard are present in the procedure, such as potential cuts from glass microscope slides and electrical hazards associated with use of the microscope
Why is it important that differential counts only be performed and reported by experienced, qualified personnel?
The results of the differential count can lead to a variety of actions such as a diagnosis, institution of treatment, or further testing. only qualified personnel allowed to perform and report differential count so that result are reliable.
Functions of Monocytes
are active in phagocytosis and in processing antigens
How is an absolute WBC count calculated?
it is obtained by multiplying the WBC count by the percentage of each type of white blood cell counted during the differential procedure