BIO UNIT 6

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Which of the following best describes the most likely impact on an individual produced from fertilization between one of the daughter cells shown and a normal gamete?

Because nondisjunction occurred in anaphase IIII, all gametes will have an abnormal chromosome number and the individual will likely exhibit phenotypic evidence of the nondisjunction event.

Figure 1. Process occurring in a cell Which of the following correctly explains the process shown in Figure 1 ?(candy wrapper looking thing)

D N A replication is occurring because replication is semi-conservative and the new strand is a copy of the template strand. Initiation of transcription is occurring because a strand of RNA is being produced from a DNA template strand

Figure 1 illustrates a model of the molecules involved in DNA replication and their placement relative to each other.Which of the following correctly explains where DNA replication will begin on the strand oriented 5'→3' , reading from left to right?

DNA replication will be initiated immediately to the left of the RNA , since DNA polymerase requires an RNA primer.

Figure 1 illustrates processes related to control of transcription and translation in a cell.Which of the following scientific claims is most consistent with the information provided in Figure 1 ?

Different genes may be regulated by the same transcription factor.

Histone methyltransferases are a class of enzymes that methylate certain amino acid sequences in histone proteins. A research team found that transcription of gene R� decreases when histone methyltransferase activity is inhibited. Which scientific claim is most consistent with these findings?

Histone methylation opens up chromatin at gene R� so transcription factors can bind to DNADNA more easily.

Both liver cells and lens cells have the genes for making the proteins albumin and crystalline. However, only liver cells express the blood protein albumin and only lens cells express crystalline, the main protein in the lens of the eye. Both of these genes have enhancer sequences associated with them. The claim that gene regulation results in differential gene expression and influences cellular products (albumin or crystalline) is best supported by evidence in which of the following statements?

Liver cells possess transcriptional activators that are different from those of lens cells.

Which of the following statements best explains the pattern seen on the gel with regard to the size and charge of molecules A and B? Responses

Molecules A and B are negatively charged, and molecule A is smaller than molecule B.

The enzyme lactase aids in the digestion of lactose, a sugar found in the milk of most mammals. In most mammal species, adults do not produce lactase. Continuing to produce lactase into adulthood in people is called lactase persistence. A number of different alleles have been identified that result in lactase persistence. Figure 1 shows the percentage of people in different geographic areas parts of the Old World that exhibit lactase persistence.

Mutations conferring lactase persistence likely arose independently in different geographic areas and offered a selective advantage.

A model of a process involving nucleic acids is shown in Figure 1.Which of the following best explains what process is represented in Figure 1 ?

New DNA strands are being synthesized in the 5' to 3' direction from their DNA templates.

Which of the following statements best explains the structure and importance of plasmids to prokaryotes?

Plasmids are circular, double-stranded DNA molecules that provide genes that may aid in survival of the prokaryotic cell.

Genetic engineering techniques can be used when analyzing and manipulating DNA and RNA. Scientists used gel electrophoresis to study transcription of gene L and discovered that mRNA strands of three different lengths are consistently produced. Which of the following explanations best accounts for this experimental result?

Pre-mRNA of gene L is subject to alternative splicing, so three mRNA sequences are possible.

Nuclear pores regulate the passage of substances into and out of the nucleus. Antibodies such as mAb414mAb414 have been used to inhibit the movement of substances through the nuclear pores of rat liver cells. Scientists cultured rat liver cells (eukaryotic) and bacteria cells (prokaryotic) in separate dishes with radioactively labeled amino acids. A specific gene in both cell types was engineered to synthesize identical polypeptide chains, and translation of this gene was measured. The procedure was repeated with mAb414mAb414 (the inhibitor) added to each of the two cell cultures, and translation was monitored again. Which of the following sets of graphs best summarizes the results of the experiments?

Prokaryotes are constant and go up; Eukaryotes are flat

Erwin Chargaff investigated the nucleotide composition of DNADNA. He analyzed DNADNA from various organisms and measured the relative amounts of adenine (AA), guanine (GG), cytosine (CC), and thymine (TT) present in the DNADNA of each organism. Table 1 contains a selected data set of his results. Which of the following statements best explains the data set?

Since the percent A and the percent G add up to approximately 50 percent in each sample, adenine and guanine molecules must pair up in a double-stranded D N A molecule. Since the %A and the %T are approximately the same in each sample, adenine and thymine molecules must pair up in a double-stranded DNA molecule.

Which of the following best describes the type of mutation shown in Figure 1 that leads to sickle-cell anemia?

Substitution

Antibiotics interfere with prokaryotic cell functions. Streptomycin is an antibiotic that affects the small ribosomal subunit in prokaryotes. Specifically, streptomycin interferes with the proper binding of tRNAtRNA to mRNAmRNA in prokaryotic ribosomes. Which of the following best predicts the most direct effect of exposing prokaryotic cells to streptomycin?

Synthesis of polypeptides will be inhibited.

Figure 1 represents part of a process essential to gene expression.Which of the following best explains what strand X represents?

The antisense strand, because it is serving as a template

Figure 1 represents part of a process that occurs in eukaryotic cells. There are untranslated regions (UTR) in this sequence.Which of the following best explains the process represented by Figure 1 ?

The enzyme-regulated processing of pre‑mRNA into mature mRNA

Antigens are foreign proteins that invade the systems of organisms. Vaccines function by stimulating an organism's immune system to develop antibodies against a particular antigen. Developing a vaccine involves producing an antigen that can be introduced into the organism being vaccinated and which will trigger an immune response without causing the disease associated with the antigen. Certain strains of bacteria can be used to produce antigens used in vaccines. Which of the following best explains how bacteria can be genetically engineered to produce a desired antigen? Responses

The gene coding for the antigen can be inserted into plasmids that can be used to transform the bacteria.

Antibiotics can be used to kill the specific pathogenic bacterium, Mycobacterium tuberculosis, that causes tuberculosis. The appearance of antibiotic-resistant strains has made it more difficult to cure M. tuberculosis infections. These antibiotic-resistant bacteria survive and pass on the genes to their offspring, making the resistant phenotype more common in the population. DNADNA analysis indicates that the genes for antibiotic resistance are not normally present in bacterial chromosomal DNADNA. Which of the following statements best explains how the genes for antibiotic resistance can be transmitted between bacteria without the exchange of bacterial chromosomal DNADNA?

The genes for antibiotic resistance are located on a plasmid that can be passed to neighboring bacteria.

Figure 1 shows some relevant details of a model of how a deoxynucleotide, in this case dTMP , is added to a growing strand of DNA. The features of this model provide evidence for which explanation of why all growing strands are synthesized in a 5′to 3′

The translation of m R N A occurs in the 5 prime to 5 prime direction; therefore, the growing D N A strand must also grow in the 5 prime to 3 prime direction. The phosphate group, attached to the 5′5′ carbon of the dTMPdTMP, forms a covalent bond with the oxygen atom attached to the 3′3′ carbon of the growing strand.

Molecular biologists are studying the processes of transcription and translation and have found that they are very similar in prokaryotes and eukaryotes, as summarized in Table 1.Based on the information in Table 1, which of the following best predicts a key difference in prokaryotes and eukaryotes with regard to transcription and translation?

The two processes will occur simultaneously in prokaryotes but not eukaryotes.

Researchers claim that bacteria that live in environments heavily contaminated with arsenic are more efficient at processing arsenic into arsenite and removing this toxin from their cells. Justify this claim based on the evidence shown in Figure 1.

There are multiple operons controlling the production of proteins that process and remove arsenite from cells in both H. arsenicoxydans and O. tritici. In contrast, E. coli has only one operon devoted to arsenic removal.

Figure 1 represents a metabolic process involving the regulation of lactose metabolism by E. coli bacteria. Lactose is utilized for energy by E. coli when glucose is not present. Allolactose is an isomer of lactose that is in the environment of these bacteria when lactose is present. The CAP site prevents the binding of RNA polymerase when glucose is present in the environment. The lacZ , lacY , and lacA genes code for proteins needed for lactose metabolism.Which is a scientific claim that is consistent with the information provided and Figure 1 ?

When bound to the operator, the repressor protein prevents lactose metabolism in E. coli.

Phytochromes are molecules that change light stimuli into chemical signals, and they are thought to target light-activated genes in plants. A study was conducted to determine how certain cell proteins were made in a plant cell using a phytochrome. Figures 1 and 2 represent findings from the study. Use the response models shown in Figures 1 and 2 to justify the claim that phytochromes regulate the transcription of genes leading to the production of certain cellular proteins.

When inactive phytochrome Pr is activated by red light to become phytochrome Pfr , it is transported into the nucleus where it binds to the transcription factor PIF3 at the promoter. This stimulates transcription, ultimately leading to protein production. Far-red light inactivates the phytochrome, which will turn transcription off by not binding to PIF3.


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