Chapter 11

What explains the increased concentration of Ca++ in the ER

Calcium ions are actively imported from the cytoplasm into the ER.

At puberty an adolescent female body changes in both structure and function of several organ systems, primarily under the influence of changing concentrations of estrogens and other steroid hormones. How can one hormone, such as estrogen, mediate so many effects,

Estrogen binds to specific receptors inside many kinds of cells, each of which have different responses to its binding.

Which of the following is a likely explanation of why natural selection favored the evolution of signals for sexual reproduction

Even in the simplest organisms, sexual reproduction required several coordinated responses by cells.

Which of the following is a correct association

GTPase activity and hydrolysis of GTP to GDP

Which observation suggested to Sutherland the involvement of a second messenger in epinephrine's effect on liver cells

Glycogen breakdown was observed only when epinephrine was administered to intact cells.

In research on aging (both cellular aging and organismal aging) it has been found that aged cells do not progress through the cell cycle as they had previously. Which of the following would provide evidence that this is related to cell signaling,

Growth factor ligands do not bind as efficiently to receptors.

Receptor tyrosine kinases (RTKs) are found at high levels on various cancer cells. A protein Herceptin, has been found to bind to an RTK known as HER2. This information can now be utilized in breast cancer treatment if which of the following is true,

If the patient's cancer cells have detectable HER2.

In C. elegans ced-9 prevents apoptosis in a normal cell in which of the following ways

It prevents the caspase activity of ced-3 and ced-4.

Which of the following is true for the signaling system in an animal cell that lacks the ability to produce GTP

It would not be able to activate and inactivate the G protein on the cytoplasmic side of the plasma membrane.

Which of the following describes the events of apoptosis

Its DNA and organelles are fragmented, the cell shrinks and forms blebs, and the cell self-digests.

Humans have receptors for two kinds of beta adrenergic compounds such as catecholamines to control cardiac muscle contractions. Some are beta 1 receptors that promote increased heart rate. Other drugs called beta blockers, slow heart rate. Smooth muscle cells, however, have beta 2 receptors, which mediate muscle relaxation. Blockers of these effects are sometimes used to treat asthma. The description above illustrates which of the following,

Just because a drug acts on one type of receptor does not mean that it will act on another type.

Why is apoptosis potentially threatening to the healthy "neighbors" of a dying cell

Lysosomal enzymes exiting the dying cell would damage surrounding cells.

Which of the following is the best explanation for the fact that most transduction pathways have multiple steps

Multiple steps provide for greater possible amplification of a signal.

In which of the following ways do plant hormones differ from hormones in animals

Plant hormones may travel in air or through vascular systems.

Which of the following statements is true of signal molecules

Protein kinase A activation is one possible result of signal molecules binding to G protein-coupled receptors.

In an experiment to track the movement of growth factor molecules from secretion to the point of receptor binding in a particular species of mammal a student found a 20-fold reduction in mm traveled when in the presence of an adrenal hormone. This is in part attributable to which of the following,

The growth factor is a paracrine signal.

Affinity chromatography is a method that can be used to purify cell-surface receptorswhile they retain their hormone-binding ability. A ligand (hormone) for a receptor of interest is chemically linked to polystyrene beads. A solubilized preparation of membrane proteins is passed over a column containing these beads. Only the receptor binds to the beads. When an excess of the ligand (hormone) is poured through the column after the receptor binding step what do you expect will occur

The ligand will cause the receptor to be displaced from the beads and eluted out.

Why has C. elegans proven to be a useful model for understanding apoptosis

The nematode undergoes a fixed and easy-to-visualize number of apoptotic events during its normal development.

Since steroid receptors are located intracellularly which of the following is true,

The steroid/receptor complex can cross the nuclear membrane.

Because most receptors are membrane proteins which of the following is usually true,

They change their conformation after binding with signal polypeptides.

Which of the following is true of transcription factors

They control gene expression.

What is most likely to happen to an animal's target cells that lack receptors for local regulators

They might not be able to multiply in response to growth factors from nearby cells.

A drug designed to inhibit the response of cells to testosterone would almost certainly result in which of the following

a decrease in transcriptional activity of certain genes

If an adult person has a faulty version of the human analog to ced-4 of the nematode which of the following is most likely to result,

a form of cancer in which there is insufficient apoptosis

Protein phosphorylation is commonly involved with all of the following except

activation of G protein-coupled receptors.

In the formation of biofilms such as those forming on unbrushed teeth, cell signaling serves which function

aggregation of bacteria that can cause cavities

Where do apoptotic signals come from

all of the above

In which of the following ways could signal transduction most probably be explored in research to treat cancer

alteration of protein kinases in cell cycle regulation in order to slow cancer growth

The human population's life expectancy has increased significantly but seems to have an upper limit. Which of the following might be described as an ecological consequence of passing that upper limit by regulating cell death

an increase in the total population of humans on the planet

A major group of G protein-coupled receptors contains seven transmembrane α helices. The amino end of the protein lies at the exterior of the plasma membrane. Loops of amino acids connect the helices either at the exterior face or on the cytosol face of the membrane. The loop on the cytosol side between helices 5 and 6 is usually substantially longer than the others. Where would you expect to find the carboxyl end

at the cytosol surface

The coupled G protein most likely interacts with this receptor

at the loop between H5 and H6.

Testosterone functions inside a cell by

binding with a receptor protein that enters the nucleus and activates specific genes.

In general a signal transmitted via phosphorylation of a series of proteins,

brings a conformational change to each protein.

Consider this pathway: epinephrine → G protein-coupled receptor → G protein → adenylyl cyclase → cAMP. Identify the second messenger.

cAMP

Caffeine is an inhibitor of phosphodiesterase. Therefore the cells of a person who has recently consumed coffee would have increased levels of,

cAMP.

The use of beta 2 antagonist drugs may be useful in asthma because they may

dilate the bronchioles by relaxing their smooth muscle.

The activation of receptor tyrosine kinases is characterized by

dimerization and phosphorylation.

Sutherland discovered that epinephrine signals

elevation of cytosolic concentrations of cyclic AMP.

One inhibitor of cGMP is Viagra. It provides a signal that leads to dilation of blood vessels and increase of blood in the penis facilitating erection. Since cGMP is inhibited, the signal is prolonged. The original signal that is now inhibited would have,

hydrolyzed cGMP to GMP.

GTPase activity is involved in the regulation of signal transduction because it

hydrolyzes GTP binding to G protein.

The function of phosphatases in signal transduction is best described as to

inactivate protein kinases and turn off the signal transduction.

The termination phase of cell signaling requires which of the following

incompatibility of the binding of the signal molecule to the receptor

Beta 2 antagonist drugs might also be used most effectively for which of the following

increasing low blood pressure

Which of the following is the best explanation for the inability of a specific animal cell to reduce the Ca2+ concentration in its cytosol compared with the extracellular fluid

insufficient ATP levels in the cytoplasm

Lipid-soluble signaling molecules such as testosterone, cross the membranes of all cells but affect only target cells because,

intracellular receptors are present only in target cells.

What are scaffolding proteins

large molecules to which several relay proteins attach to facilitate cascade effects

Binding of a signaling molecule to which type of receptor leads directly to a change in the distribution of ions on opposite sides of the membrane

ligand-gated ion channel

Apoptosis involves all but which of the following

lysis of the cell

The toxin of Vibrio cholerae causes profuse diarrhea because it

modifies a G protein involved in regulating salt and water secretion.

This method of affinity chromatography would be expected to collect which of the following

molecules of purified receptor

Which of the following is characterized by a cell releasing a signal molecule into the followed by a number of cells in the immediate vicinity responding,

paracrine signaling

Adenylyl cyclase has the opposite effect of which of the following

phosphodiesterase

An inhibitor of which of the following could be used to block the release of calcium from the endoplasmic reticulum

phospholipase C

An inhibitor of phosphodiesterase activity would have which of the following effects

prolong the effect of epinephrine by maintaining elevated cAMP levels in the cytoplasm

Which of the following most likely would be an immediate result of growth factor binding to its receptor

protein kinase activity

Which of the following would be inhibited by a drug that specifically blocks the addition of phosphate groups to proteins

receptor tyrosine kinase activity

One of the major categories of receptors in the plasma membrane reacts by forming dimers adding phosphate groups, and then activating relay proteins. Which type does this,

receptor tyrosine kinases

The receptors for a group of signaling molecules known as growth factors are often

receptor tyrosine kinases.

Which of the following amino acids are most frequently phosphorylated by protein kinases in the cytoplasm during signal transduction

serine and threonine

When a neuron responds to a particular neurotransmitter by opening gated ion channels the neurotransmitter is serving as which part of the signal pathway

signal molecule

Which of the following types of signaling is represented in the figure

synaptic

If a pharmaceutical company wished to design a drug to maintain low blood sugar levels one approach might be to design a compound,

that increases phosphodiesterase activity.

Of the following a receptor protein in a membrane that recognizes a chemical signal is most similar to,

the active site of an allosteric enzyme that binds to a specific substrate

If you wish to design an experiment to block the G protein-coupled receptor interaction the block would preferentially affect which of the following

the amino acid sequence in the binding site for the G protein

In the figure

the dots in the space between the two structures represent which of the following, neurotransmitters

In yeast signal transduction

the yeast cells, secrete molecules that result in response by other yeast cells.

Phosphorylation cascades involving a series of protein kinases are useful for cellular signal transduction because

they amplify the original signal manyfold.

If a pharmaceutical company wished to design a drug to maintain low blood sugar levels one approach might be to design a compound,

to block G protein activity in liver cells.

Using the yeast signal transduction pathways both types of mating cells release the mating factors. These factors bind to specific receptors on the correct cells,

which induce changes in the cells that lead to cell fusion.