Chapter 2 lec.- Microbiology 2420

Label the figure to demonstrate your understanding of selective and differential media.

(Left) Mixed sample> (light yellow) General-Purpose Medium (different species look similar), (red) Differential Medium (Right) Mixed sample> (light yellow) General-Purpose Medium (many different species grow), (yellow-orange) Selective Medium

Match the staining technique to the appropriate example.

1. Methylene blue- Simple stain 2. Gram stain- Differential stain 3. Endoscope stain- Differential stain 4. Flagellar stain- Special stain

Choose the differential stain that is most important in the diagnosis of tuberculosis.

Acid-fast stain

Some organisms ferment certain carbohydrates to produce an acidic byproduct while others don't. What component of mannitol salt agar makes this media differential?

Both the mannitol and the phenol red

A fastidious organism must be grown on/in what type of medium?

Enriched medium

Review the five I's of microbiology laboratory by completing each sentence.

INOCULATION involves placing a sample into a container of fresh medium. Multiplication of an organism in a culture is promoted by INCUBATION. Pour plating, streak plating, and spread plating are ISOLATION methods for separating individual microbes from each other. INSPECTION involves the observation of macroscopic and microscopic characteristics of microbes in samples. Biochemical, immunological, and genetic testing assist the microbiologist in INFORMATION GATHERING so that a final IDENTIFICATION can be made.

In a ___ stain, the dye stains the background, forming a silhouette around the unstained organism.

Negative

Drag and drop the labels into the correct empty boxes to complete the concept map.

Specimen collection from a SOURCE OF MICROBES carried to the lab in a TRANSPORT MEDIUM >>>>> (left to right) INOCULATION> in a sterile MEDIUM> such as STREAK PLATING INCUBATION> image> promotes MULTIPLICATION ISOLATION> image> SUBCULTURING> to obtain a specimen containing a single species> image INSPECTION> both macroscopically> image> and microscopically via STAINING IDENTIFICATION> using BIOCHEMICAL TESTS genetic analysis and immunologic tests