Chapter 6 Success Procedures and Biochemical Identification of Bacteria

334. The ability of a microorganism to deaminate phenylalanine can be assessed by inoculating a phenylalanine agar slant with the test organism. Following incubation, if the organism is positive, a green color develops with the addition of A. 10% ferric chloride B. 2% sulfanilamide C. 2 N sodium carbonate D. 5% alpha-naphthol

A. 10% ferric chloride

338. The Moeller test for the detection of decarboxylase activity is dependent upon A. An alkaline pH shift in the medium B. The oxidation of gluconate C. An acid pH basal medium D. A deamination of tryptophan

A. An alkaline pH shift in the medium

343. Which of the following statements does not apply to the acridine orange stain? A. Binds to the teichoic acid of the cell wall B. Requires the use of a fluorescence microscope C. Is more sensitive than the Gram stain D. Is recommended for fluid and exudates with low bacterial concentrations

A. Binds to the teichoic acid of the cell wall

333. Laboratory professionals are at risk for disease transmission. The majority of cases of laboratory-related infections are associated with A. Infectious aerosols B. Contamination of abraded skin C. Puncture wounds D. Person-to-person transmission

A. Infectious aerosols

344. In the catalase test, the formation of bubbles is due to A. Production of oxygen (O2) B. Production of hydrogen gas (H2) C. Breakdown of water D. Oxidation of hydrogen peroxide

A. Production of oxygen (O2)

332. Suggested quality control organisms for Simmons citrate agar slants are A. Shigella sonnet; Escherichia coli B. Klebsiella pneumoniae; Escherichia coli C. Escherichia coli; Edwardsiella tarda D. Morganella morganii; Edwardsiella tarda

B. Klebsiella pneumoniae; Escherichia coli

327. During childbearing years, the normal flora of the vagina is predominantly A. Enterococcus B. Lactobacillus C. Propionibacterium D. Coagulase-negative Staphylococcus

B. Lactobacillus

336. The paper strip test for the demonstration of hydrogen sulfide production is impregnated with a solution of A. Sodium desoxycholate B. Lead acetate C. Potassium tellurite D. Sodium thiosulfate

B. Lead acetate

339. The medium used to determine whether an organism is oxidative or fermentative with respect to its metabolic activities is A. CTA medium B. OF medium C. HE medium D. XLD medium

B. OF medium

331. The recommended anticoagulant for use when a body fluid or joint fluid that may clot is sent for microbiologic examination is A. Heparin B. Sodium polyethanolsulfonate (SPS) C. Sodium EDTA D. Sodium citrate

B. Sodium polyethanolsulfonate (SPS)

345. Which of the following would not be appropriate for a positive control in the PYR test? A. Staphylococcus aureus B. Staphylococcus lugdunensis C. Streptococcus pyogenes D. Enterococcusfaecalis

B. staphylococcus lugdunensis

328. The MIDI identification system is based on A. Antibiograms B. Colony pigment on ChromAgar C. Fatty acid analysis D. Multiple biochemical tests

C. Fatty acid analysis

335. Which of the following media is both selective and differential? A. Sheep blood agar B. Chocolate agar C. Mannitol salt agar D. Mueller-Hinton agar

C. Mannitol salt agar

326. Which of the following body sites is not normally colonized by large numbers of normal flora organisms? A. Colon B. Skin C. Lungs D. Vagina

C. lungs

340. Methyl red-Voges Proskauer (MRVP) broth is inoculated and incubated for 48 hours. What two reagents must be added to determine if the bacterium is VP positive? A. Creatine and 1N HC1 B. 10% FeCl3 and alpha-naphthol C. Kovac's reagent and zinc dust D. Alpha-naphthol and 40% KOH

D. Alpha-naphthol and 40% KOH

337. In the nitrate test, reduction is demonstrated by the development of a red color, following the addition of A. Alpha-naphthol and potassium hydroxide B. p-Dimethylaminobenzaldehyde and amyl alcohol C. Ninhydrin and acetone D. Alpha-naphthylamine and sulfanilic acid

D. Alpha-naphthylamine and sulfanilic acid

329. Which of the following is not a correct statement regarding blood cultures? A. Collection of 10-20 mL per culture for adults is recommended B. Two or three blood cultures are recommended as optimum C. Volume of blood cultured is more critical than timing of culture D. Blood drawn for culture may be allowed to clot

D. Blood drawn for culture may be allowed to clot

341. Which of the following is not true of blood cultures for the recovery of bacteria? A. No more than three cultures should be drawn in 1 day. B. Cultures should be drawn before the expected fever spike. C. Cultures should be incubated aerobically and anaerobically. D. Collect 5 mL of blood for optimal recovery of pathogen.

D. Collect 5 mL of blood for optimal recovery of pathogen

342. Continuous blood culture systems that detect changes in headspace pressure in bottles are refered to as A. Fluorescence methods B. 14C detection methods C. Colorimetry methods D. Manometric methods

D. Manometric methods

330. In capnophilic incubators, carbon dioxide concentrations should be maintained between A. I%and5% B. 5% and 10% C. 10% and 15% D. 15% and 20%

b. 5%-10%