Chapter 9 Cellular Respiration/Fermentation

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29) A young animal has never had much energy. He is brought to a veterinarian for help and is sent to the animal hospital for some tests. There they discover his mitochondria can use only fatty acids and amino acids for respiration, and his cells produce more lactate than normal. Of the following, which is the best explanation of his condition? A) His mitochondria lack the transport protein that moves pyruvate across the outer mitochondrial membrane. B) His cells cannot move NADH from glycolysis into the mitochondria. C) His cells contain something that inhibits oxygen use in his mitochondria. D) His cells lack the enzyme in glycolysis that forms pyruvate. E) His cells have a defective electron transport chain, so glucose goes to lactate instead of to acetyl CoA.

A

5) Which of the following statements describes the results of this reaction? C6H12O6 + 6 O2 → 6 CO2 + 6 H2O + Energy A) C6H12O6 is oxidized and O2 is reduced. B) O2 is oxidized and H2O is reduced. C) CO2 is reduced and O2 is oxidized. D) C6H12O6 is reduced and CO2 is oxidized. E) O2 is reduced and CO2 is oxidized.

A

57) Which of the following occurs in the cytosol of a eukaryotic cell? A) glycolysis and fermentation B) fermentation and chemiosmosis C) oxidation of pyruvate to acetyl CoA D) citric acid cycle E) oxidative phosphorylation

A

60) In the absence of oxygen, yeast cells can obtain energy by fermentation, resulting in the production of A) ATP, CO2, and ethanol (ethyl alcohol). B) ATP, CO2, and lactate. C) ATP, NADH, and pyruvate. D) ATP, pyruvate, and oxygen. E) ATP, pyruvate, and acetyl CoA.

A

64) Which statement best supports the hypothesis that glycolysis is an ancient metabolic pathway that originated before the last universal common ancestor of life on Earth? A) Glycolysis is widespread and is found in the domains Bacteria, Archaea, and Eukarya. B) Glycolysis neither uses nor needs O2. C) Glycolysis is found in all eukaryotic cells. D) The enzymes of glycolysis are found in the cytosol rather than in a membrane-enclosed organelle. E) Ancient prokaryotic cells, the most primitive of cells, made extensive use of glycolysis long before oxygen was present in Earth's atmosphere.

A

69) A mutation in yeast makes it unable to convert pyruvate to ethanol. How will this mutation affect these yeast cells? A) The mutant yeast will be unable to grow anaerobically. B) The mutant yeast will grow anaerobically only when given glucose. C) The mutant yeast will be unable to metabolize glucose. D) The mutant yeast will die because they cannot regenerate NAD+ from NAD. E) The mutant yeast will metabolize only fatty acids.

A

70) You have a friend who lost 7 kg (about 15 pounds) of fat on a regimen of strict diet and exercise. How did the fat leave her body? A) It was released as CO2 and H2O. B) It was converted to heat and then released. C) It was converted to ATP, which weighs much less than fat. D) It was broken down to amino acids and eliminated from the body. E) It was converted to urine and eliminated from the body.

A

77) What carbon sources can yeast cells metabolize to make ATP from ADP under anaerobic conditions? A) glucose B) ethanol C) pyruvate D) lactic acid E) either ethanol or lactic acid

A

8) Which of the following statements describes NAD+? A) NAD+ is reduced to NADH during glycolysis, pyruvate oxidation, and the citric acid cycle. B) NAD+ has more chemical energy than NADH. C) NAD+ is oxidized by the action of hydrogenases. D) NAD+ can donate electrons for use in oxidative phosphorylation. E) In the absence of NAD+, glycolysis can still function.

A

80) Yeast cells that have defective mitochondria incapable of respiration will be able to grow by catabolizing which of the following carbon sources for energy? A) glucose B) proteins C) fatty acids D) glucose, proteins, and fatty acids E) Such yeast cells will not be capable of catabolizing any food molecules, and will therefore die.

A

84) Which portion of the pathway in Figure 9.1 involves an endergonic reaction? A) A B) B C) C D) D E) E

A

85) Which portion of the pathway in Figure 9.1 contains a phosphorylation reaction in which ATP is the phosphate source? A) A B) B C) C D) D E) E

A

86) Starting with one molecule of isocitrate and ending with fumarate, how many ATP molecules can be made through substrate-level phosphorylation (see Figure 9.2)? A) 1 B) 2 C) 11 D) 12 E) 24

A

1) What is the term for metabolic pathways that release stored energy by breaking down complex molecules? A) anabolic pathways B) catabolic pathways C) fermentation pathways D) thermodynamic pathways E) bioenergetic pathways

B

11) The oxygen consumed during cellular respiration is involved directly in which process or event? A) glycolysis B) accepting electrons at the end of the electron transport chain C) the citric acid cycle D) the oxidation of pyruvate to acetyl CoA E) the phosphorylation of ADP to form ATP

B

12) Which process in eukaryotic cells will proceed normally whether oxygen (O2) is present or absent? A) electron transport B) glycolysis C) the citric acid cycle D) oxidative phosphorylation E) chemiosmosis

B

19) Starting with one molecule of glucose, the energy-containing products of glycolysis are A) 2 NAD+, 2 pyruvate, and 2 ATP. B) 2 NADH, 2 pyruvate, and 2 ATP. C) 2 FADH2, 2 pyruvate, and 4 ATP. D) 6 CO2, 2 ATP, and 2 pyruvate. E) 6 CO2, 30 ATP, and 2 pyruvate.

B

2) The molecule that functions as the reducing agent (electron donor) in a redox or oxidation-reduction reaction A) gains electrons and gains potential energy. B) loses electrons and loses potential energy. C) gains electrons and loses potential energy. D) loses electrons and gains potential energy. E) neither gains nor loses electrons, but gains or loses potential energy.

B

20) In glycolysis, for each molecule of glucose oxidized to pyruvate A) two molecules of ATP are used and two molecules of ATP are produced. B) two molecules of ATP are used and four molecules of ATP are produced. C) four molecules of ATP are used and two molecules of ATP are produced. D) two molecules of ATP are used and six molecules of ATP are produced. E) six molecules of ATP are used and six molecules of ATP are produced.

B

28) Carbon dioxide (CO2) is released during which of the following stages of cellular respiration? A) glycolysis and the oxidation of pyruvate to acetyl CoA B) oxidation of pyruvate to acetyl CoA and the citric acid cycle C) the citric acid cycle and oxidative phosphorylation D) oxidative phosphorylation and fermentation E) fermentation and glycolysis

B

30) During aerobic respiration, electrons travel downhill in which sequence? A) food → citric acid cycle → ATP → NAD+ B) food → NADH → electron transport chain → oxygen C) glucose → pyruvate → ATP → oxygen D) glucose → ATP → electron transport chain → NADH E) food → glycolysis → citric acid cycle → NADH → ATP

B

33) In cellular respiration, the energy for most ATP synthesis is supplied by A) high energy phosphate bonds in organic molecules. B) a proton gradient across a membrane. C) converting oxygen to ATP. D) transferring electrons from organic molecules to pyruvate. E) generating carbon dioxide and oxygen in the electron transport chain.

B

35) The primary role of oxygen in cellular respiration is to A) yield energy in the form of ATP as it is passed down the respiratory chain. B) act as an acceptor for electrons and hydrogen, forming water. C) combine with carbon, forming CO2. D) combine with lactate, forming pyruvate. E) catalyze the reactions of glycolysis.

B

47) The synthesis of ATP by oxidative phosphorylation, using the energy released by movement of protons across the membrane down their electrochemical gradient, is an example of A) active transport. B) an endergonic reaction coupled to an exergonic reaction. C) a reaction with a positive ΔG . D) osmosis. E) allosteric regulation.

B

50) What is proton-motive force? A) the force required to remove an electron from hydrogen B) the force exerted on a proton by a transmembrane proton concentration gradient C) the force that moves hydrogen into the intermembrane space D) the force that moves hydrogen into the mitochondrion E) the force that moves hydrogen to NAD+

B

65) Why is glycolysis considered to be one of the first metabolic pathways to have evolved? A) It produces much less ATP than does oxidative phosphorylation. B) It does not involve organelles or specialized structures, does not require oxygen, and is present in most organisms. C) It is found in prokaryotic cells but not in eukaryotic cells. D) It relies on chemiosmosis, which is a metabolic mechanism present only in the first cells' prokaryotic cells. E) It requires the presence of membrane-enclosed cell organelles found only in eukaryotic cells.

B

81) Which step in Figure 9.1 shows a split of one molecule into two smaller molecules? A) A B) B C) C D) D E) E

B

16) During glycolysis, when each molecule of glucose is catabolized to two molecules of pyruvate, most of the potential energy contained in glucose is A) transferred to ADP, forming ATP. B) transferred directly to ATP. C) retained in the two pyruvates. D) stored in the NADH produced. E) used to phosphorylate fructose to form fructose 6-phosphate.

C

17) In addition to ATP, what are the end products of glycolysis? A) CO2 and H2O B) CO2 and pyruvate C) NADH and pyruvate D) CO2 and NADH E) H2O, FADH2, and citrate

C

18) The free energy for the oxidation of glucose to CO2 and water is -686 kcal/mol and the free energy for the reduction of NAD+ to NADH is +53 kcal/mol. Why are only two molecules of NADH formed during glycolysis when it appears that as many as a dozen could be formed? A) Most of the free energy available from the oxidation of glucose is used in the production of ATP in glycolysis. B) Glycolysis is a very inefficient reaction, with much of the energy of glucose released as heat. C) Most of the free energy available from the oxidation of glucose remains in pyruvate, one of the products of glycolysis. D) There is no CO2 or water produced as products of glycolysis. E) Glycolysis consists of many enzymatic reactions, each of which extracts some energy from the glucose molecule.

C

22) Which kind of metabolic poison would most directly interfere with glycolysis? A) an agent that reacts with oxygen and depletes its concentration in the cell B) an agent that binds to pyruvate and inactivates it C) an agent that closely mimics the structure of glucose but is not metabolized D) an agent that reacts with NADH and oxidizes it to NAD+ E) an agent that blocks the passage of electrons along the electron transport chain

C

32) Where are the proteins of the electron transport chain located? A) cytosol B) mitochondrial outer membrane C) mitochondrial inner membrane D) mitochondrial intermembrane space E) mitochondrial matrix

C

37) During aerobic respiration, H2O is formed. Where does the oxygen atom for the formation of the water come from? A) carbon dioxide (CO2) B) glucose (C6H12O6) C) molecular oxygen (O2) D) pyruvate (C3H3O3-) E) lactate (C3H5O3-)

C

44) How many oxygen molecules (O2) are required each time a molecule of glucose (C6H12O6) is completely oxidized to carbon dioxide and water via aerobic respiration,? A) 1 B) 3 C) 6 D) 12 E) 30

C

51) In liver cells, the inner mitochondrial membranes are about five times the area of the outer mitochondrial membranes. What purpose must this serve? A) It allows for an increased rate of glycolysis. B) It allows for an increased rate of the citric acid cycle. C) It increases the surface for oxidative phosphorylation. D) It increases the surface for substrate-level phosphorylation. E) It allows the liver cell to have fewer mitochondria.

C

6) When a glucose molecule loses a hydrogen atom as the result of an oxidation-reduction reaction, the molecule becomes A) hydrolyzed. B) hydrogenated. C) oxidized. D) reduced. E) an oxidizing agent.

C

66) When an individual is exercising heavily and when the muscle becomes oxygen-deprived, muscle cells convert pyruvate to lactate. What happens to the lactate in skeletal muscle cells? A) It is converted to NAD+. B) It produces CO2 and water. C) It is taken to the liver and converted back to pyruvate. D) It reduces FADH2 to FAD+. E) It is converted to alcohol.

C

7) When a molecule of NAD+ (nicotinamide adenine dinucleotide) gains a hydrogen atom (not a proton), the molecule becomes A) dehydrogenated. B) oxidized. C) reduced. D) redoxed. E) hydrolyzed.

C

82) In which step in Figure 9.1 is an inorganic phosphate added to the reactant? A) A B) B C) C D) D E) E

C

14) Why are carbohydrates and fats considered high energy foods? A) They have a lot of oxygen atoms. B) They have no nitrogen in their makeup. C) They can have very long carbon skeletons. D) They have a lot of electrons associated with hydrogen. E) They are easily reduced.

D

21) A molecule that is phosphorylated A) has been reduced as a result of a redox reaction involving the loss of an inorganic phosphate. B) has a decreased chemical reactivity; it is less likely to provide energy for cellular work. C) has been oxidized as a result of a redox reaction involving the gain of an inorganic phosphate. D) has an increased chemical potential energy; it is primed to do cellular work. E) has less energy than before its phosphorylation and therefore less energy for cellular work.

D

25) Which of the following intermediary metabolites enters the citric acid cycle and is formed, in part, by the removal of a carbon (CO2) from one molecule of pyruvate? A) lactate B) glyceraldehydes-3-phosphate C) oxaloacetate D) acetyl CoA E) citrate

D

38) In chemiosmotic phosphorylation, what is the most direct source of energy that is used to convert ADP + i to ATP? A) energy released as electrons flow through the electron transport system B) energy released from substrate-level phosphorylation C) energy released from movement of protons through ATP synthase, against the electrochemical gradient D) energy released from movement of protons through ATP synthase, down the electrochemical gradient E) No external source of energy is required because the reaction is exergonic.

D

39) Energy released by the electron transport chain is used to pump H+ into which location in eukaryotic cells? A) cytosol B) mitochondrial outer membrane C) mitochondrial inner membrane D) mitochondrial intermembrane space E) mitochondrial matrix

D

42) Where is ATP synthase located in the mitochondrion? A) cytosol B) electron transport chain C) outer membrane D) inner membrane E) mitochondrial matrix

D

48) Chemiosmotic ATP synthesis (oxidative phosphorylation) occurs in A) all cells, but only in the presence of oxygen. B) only eukaryotic cells, in the presence of oxygen. C) only in mitochondria, using either oxygen or other electron acceptors. D) all respiring cells, both prokaryotic and eukaryotic, using either oxygen or other electron acceptors. E) all cells, in the absence of respiration.

D

58) Which metabolic pathway is common to both cellular respiration and fermentation? A) the oxidation of pyruvate to acetyl CoA B) the citric acid cycle C) oxidative phosphorylation D) glycolysis E) chemiosmosis

D

73) Even though plants carry on photosynthesis, plant cells still use their mitochondria for oxidation of pyruvate. When and where will this occur? A) in photosynthetic cells in the light, while photosynthesis occurs concurrently B) in nonphotosynthesizing cells only C) in cells that are storing glucose only D) in all cells, all the time E) in photosynthesizing cells in the light and in other tissues in the dark

D

94) Which of the protein complexes labeled with Roman numerals in Figure 9.3 will transfer electrons to O2? A) complex I B) complex II C) complex III D) complex IV E) All of the complexes can transfer electrons to O2.

D

23) Why is glycolysis described as having an investment phase and a payoff phase? A) It both splits molecules and assembles molecules. B) It attaches and detaches phosphate groups. C) It uses glucose and generates pyruvate. D) It shifts molecules from cytosol to mitochondrion. E) It uses stored ATP and then forms a net increase in ATP.

E

34) During aerobic respiration, which of the following directly donates electrons to the electron transport chain at the lowest energy level? A) NAD+ B) STP C) ATP D) ADP + i E) FADH2

E

36) Inside an active mitochondrion, most electrons follow which pathway? A) glycolysis → NADH → oxidative phosphorylation → ATP → oxygen B) citric acid cycle → FADH2 → electron transport chain → ATP C) electron transport chain → citric acid cycle → ATP → oxygen D) pyruvate → citric acid cycle → ATP → NADH → oxygen E) citric acid cycle → NADH → electron transport chain → oxygen

E

45) Which of the following produces the most ATP when glucose (C6H12O6) is completely oxidized to carbon dioxide (CO2) and water? A) glycolysis B) fermentation C) oxidation of pyruvate to acetyl CoA D) citric acid cycle E) oxidative phosphorylation (chemiosmosis)

E

46) Approximately how many molecules of ATP are produced from the complete oxidation of two molecules of glucose (C6H12O6) in aerobic cellular respiration? A) 2 B) 4 C) 15 D) 30-32 E) 60-64

E

55) Which catabolic processes may have been used by cells on ancient Earth before free oxygen became available? A) glycolysis and fermentation only B) glycolysis and the citric acid cycle only C) glycolysis, pyruvate oxidation, and the citric acid cycle D) oxidative phosphorylation only E) glycolysis, pyruvate oxidation, the citric acid cycle, and oxidative phosphorylation, using an electron acceptor other than oxygen

E

63) An organism is discovered that thrives both in the presence and absence of oxygen in the air. Curiously, the consumption of sugar increases as oxygen is removed from the organism's environment, even though the organism does not gain much weight. This organism A) must use a molecule other than oxygen to accept electrons from the electron transport chain. B) is a normal eukaryotic organism. C) is photosynthetic. D) is an anaerobic organism. E) is a facultative anaerobe.

E

9) Where does glycolysis take place in eukaryotic cells? A) mitochondrial matrix B) mitochondrial outer membrane C) mitochondrial inner membrane D) mitochondrial intermembrane space E) cytosol

E


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